Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MNU25_RS10955 Genome accession   NZ_LR746496
Coordinates   2469024..2470082 (+) Length   352 a.a.
NCBI ID   WP_240985100.1    Uniprot ID   A0A8S0WP09
Organism   Acididesulfobacillus acetoxydans isolate PacBioINE     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2464024..2475082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNU25_RS10930 (DEACI_2256) - 2465089..2465523 (+) 435 WP_240985095.1 type II secretion system protein GspG -
  MNU25_RS10935 (DEACI_2257) - 2465520..2465954 (+) 435 WP_240985096.1 type IV pilus modification PilV family protein -
  MNU25_RS10940 (DEACI_2258) - 2466110..2466589 (+) 480 WP_240985097.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MNU25_RS10945 (DEACI_2259) - 2466651..2467157 (+) 507 WP_240985098.1 hypothetical protein -
  MNU25_RS10950 (DEACI_2260) - 2467159..2469027 (+) 1869 WP_240985099.1 GspE/PulE family protein -
  MNU25_RS10955 (DEACI_2261) pilT 2469024..2470082 (+) 1059 WP_240985100.1 type IV pilus twitching motility protein PilT Machinery gene
  MNU25_RS10960 (DEACI_2262) - 2470383..2471582 (+) 1200 WP_240985101.1 type II secretion system F family protein -
  MNU25_RS10965 (DEACI_2263) - 2471699..2472232 (+) 534 WP_240985102.1 type II secretion system protein -
  MNU25_RS10970 (DEACI_2264) - 2472396..2473154 (+) 759 WP_240985103.1 prepilin peptidase -
  MNU25_RS10975 (DEACI_2265) - 2473171..2474556 (+) 1386 WP_240985104.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38387.18 Da        Isoelectric Point: 7.5218

>NTDB_id=1129867 MNU25_RS10955 WP_240985100.1 2469024..2470082(+) (pilT) [Acididesulfobacillus acetoxydans isolate PacBioINE]
MMAVEELLRLAGEAKASDIHLSVESPPVFRVDGALLPLSEDKLSRTDVEEFAARLLNQVQAAKFAESGELDFSFGLPGVG
RYRMNAFRQRGSVGMVIRLIPFESPAPEALGLPRVAVDLANLGRGLVLVTGPTGSGKSTTLASLIDRINRTRACHIVTIE
DPIEYLHRHQKSLVNQREVGNDTRSFATALRAVLRQDPDVILVGEMRDLETMSTAITAAETGHLVFSTLHTNDSTQAVDR
MIDVFPPYQQQQIRVQLAAVLQGILAQQLLPRANRRGRVAAIEVLLATPAVRNLIREGKTHQLATVLQTGGKLGMQTMDK
AITDLLRQGIITPETAAERMVNPDGLRNAPGF

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1129867 MNU25_RS10955 WP_240985100.1 2469024..2470082(+) (pilT) [Acididesulfobacillus acetoxydans isolate PacBioINE]
ATGATGGCGGTGGAGGAACTTTTACGTTTAGCCGGGGAGGCAAAGGCTTCGGACATACATCTCTCTGTGGAAAGTCCCCC
GGTCTTTCGGGTGGATGGCGCTTTACTTCCCTTATCAGAGGACAAGCTGAGCCGGACAGATGTCGAGGAGTTCGCCGCTC
GCTTGTTAAATCAGGTTCAAGCCGCGAAATTCGCCGAGTCGGGAGAGTTGGATTTCTCCTTCGGTTTGCCCGGGGTCGGC
CGCTACAGGATGAATGCCTTCAGGCAGAGGGGCTCGGTGGGCATGGTTATTCGTCTCATCCCTTTTGAAAGTCCGGCGCC
GGAAGCTTTGGGCCTGCCGCGGGTGGCGGTGGATCTGGCTAATCTGGGGAGAGGGCTGGTTTTGGTTACGGGGCCGACGG
GGAGCGGCAAGTCGACGACTCTGGCCTCGCTAATCGACCGGATAAACCGAACCCGGGCTTGCCATATCGTCACGATCGAG
GATCCGATTGAGTATTTGCATCGTCATCAGAAGAGCCTGGTCAACCAGAGGGAAGTGGGCAATGACACTCGATCCTTTGC
CACTGCCCTACGGGCGGTCTTGAGACAAGACCCGGACGTCATTCTCGTGGGCGAAATGCGGGACTTAGAAACCATGAGTA
CCGCCATCACGGCGGCAGAAACCGGACATCTGGTTTTCAGCACCTTGCACACGAATGACTCAACCCAGGCGGTGGATCGT
ATGATCGATGTCTTTCCCCCCTATCAGCAGCAGCAGATTCGGGTCCAGTTGGCCGCGGTTCTGCAGGGAATTTTGGCCCA
GCAACTCTTGCCCAGAGCCAATCGGCGCGGAAGGGTCGCAGCGATCGAAGTACTCTTGGCTACCCCCGCGGTGCGCAATC
TTATCCGGGAGGGAAAAACCCATCAGCTGGCGACTGTCTTGCAGACGGGCGGAAAACTGGGCATGCAGACCATGGATAAG
GCCATCACCGATCTCCTGCGCCAAGGGATAATCACCCCCGAAACCGCCGCAGAGAGGATGGTAAATCCGGACGGCCTGCG
CAATGCCCCCGGTTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.294

96.591

0.534
  pilT Legionella pneumophila strain Lp02

50.147

96.875

0.486
  pilT Legionella pneumophila strain ERS1305867

50.147

96.875

0.486
  pilT Neisseria meningitidis 8013

48.696

98.011

0.477
  pilT Pseudomonas stutzeri DSM 10701

48.696

98.011

0.477
  pilT Pseudomonas aeruginosa PAK

48.696

98.011

0.477
  pilT Neisseria gonorrhoeae MS11

48.406

98.011

0.474
  pilT Acinetobacter baumannii strain A118

47.826

98.011

0.469
  pilT Acinetobacter nosocomialis M2

47.826

98.011

0.469
  pilT Acinetobacter baumannii D1279779

47.826

98.011

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.083

96.307

0.463
  pilT Vibrio cholerae strain A1552

48.083

96.307

0.463
  pilT Acinetobacter baylyi ADP1

47.246

98.011

0.463
  pilU Pseudomonas stutzeri DSM 10701

45.97

95.17

0.437
  pilU Vibrio cholerae strain A1552

42.943

94.602

0.406
  pilU Acinetobacter baylyi ADP1

40.346

98.58

0.398

Multiple sequence alignment