Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FXZ10_RS09870 Genome accession   NZ_LR698965
Coordinates   1989733..1990773 (-) Length   346 a.a.
NCBI ID   WP_052651876.1    Uniprot ID   A0A098EMP4
Organism   Planococcus massiliensis isolate MGYG-HGUT-01482     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1984733..1995773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXZ10_RS09845 - 1985226..1986188 (-) 963 WP_110925618.1 LysM peptidoglycan-binding domain-containing protein -
  FXZ10_RS09850 - 1986185..1986949 (-) 765 WP_052651872.1 PilN domain-containing protein -
  FXZ10_RS09855 pilM 1986952..1987890 (-) 939 WP_052651873.1 type IV pilus biogenesis protein PilM -
  FXZ10_RS09860 - 1987922..1988383 (-) 462 WP_052651874.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FXZ10_RS09865 - 1988518..1989729 (-) 1212 WP_052651875.1 type II secretion system F family protein -
  FXZ10_RS09870 pilT 1989733..1990773 (-) 1041 WP_052651876.1 type IV pilus twitching motility protein PilT Machinery gene
  FXZ10_RS09875 - 1990776..1992434 (-) 1659 WP_199876607.1 GspE/PulE family protein -
  FXZ10_RS09880 - 1992456..1993943 (-) 1488 WP_052651877.1 VanW family protein -
  FXZ10_RS09885 - 1993945..1994334 (-) 390 WP_052651878.1 hypothetical protein -
  FXZ10_RS09890 - 1994676..1995092 (+) 417 WP_052651879.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FXZ10_RS09895 - 1995079..1995618 (+) 540 WP_052651880.1 type II secretion system protein J -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38061.73 Da        Isoelectric Point: 6.5789

>NTDB_id=1128718 FXZ10_RS09870 WP_052651876.1 1989733..1990773(-) (pilT) [Planococcus massiliensis isolate MGYG-HGUT-01482]
MPNDYINKVLTAAQEKNVSDIHITVGIPPVFRMHGTLQRLGEESITPELSREIGKALIPEKLWDVFLEKGEMDYSYSLPG
VARFRVNSFQQRGSISHAFRMIPTGVPTIEDLNMPPTLKNLAEINQGLILVTGPTGSGKSTTLAAMIRHINEKMNKHIIT
LEDPIEYLHQHGTSIIDQREVGFDTGSFANGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVMATLHTSSAASTI
ERIIDVFPPEQQSQVRTQLGGVLKAVIAQRLLPTADNKGRVAATEIMINNTAIANLIRTEKVHQIPNVILTNRSAGMHMM
LTTIQNLLSQGKISRQTAQPFLEGEE

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1128718 FXZ10_RS09870 WP_052651876.1 1989733..1990773(-) (pilT) [Planococcus massiliensis isolate MGYG-HGUT-01482]
ATGCCAAATGACTATATTAACAAAGTATTAACTGCTGCCCAGGAAAAAAATGTATCGGATATTCACATCACAGTCGGAAT
TCCACCGGTCTTTCGGATGCACGGAACGCTTCAGCGATTAGGGGAGGAAAGCATCACACCGGAACTTTCAAGAGAAATAG
GAAAAGCATTAATTCCCGAGAAGCTATGGGACGTTTTTCTAGAAAAAGGCGAGATGGACTACTCCTACTCGTTACCTGGA
GTTGCCAGATTCCGAGTCAACTCATTCCAGCAGCGTGGCTCGATTTCCCATGCATTCCGGATGATTCCGACCGGCGTTCC
GACAATTGAAGATTTAAACATGCCGCCGACCTTAAAAAACTTGGCTGAAATCAACCAAGGGTTGATTTTGGTGACGGGGC
CAACGGGATCTGGCAAGTCGACGACGCTTGCTGCAATGATCCGCCACATCAATGAAAAAATGAATAAGCATATCATAACG
CTGGAAGATCCGATTGAATATTTGCATCAGCACGGGACGTCAATCATTGACCAACGGGAAGTCGGCTTTGACACCGGCAG
TTTCGCAAATGGCTTGCGGGCGGCATTGCGCCAAGATCCAGACGTGATCTTAGTCGGAGAAATGCGTGACCTTGAAACCA
TTTCAACTGCCATCACAGCTGCCGAAACGGGCCACTTGGTCATGGCGACACTCCATACGTCGAGTGCCGCGTCAACGATT
GAACGGATTATCGACGTCTTTCCGCCGGAACAGCAATCCCAAGTACGTACGCAACTTGGCGGTGTCTTAAAAGCGGTCAT
TGCACAGCGGTTGCTGCCGACTGCGGACAACAAAGGACGCGTCGCCGCTACGGAAATCATGATCAATAACACGGCCATCG
CCAATTTGATCCGCACGGAAAAAGTGCATCAAATTCCGAACGTGATTTTGACGAACCGTTCTGCGGGCATGCATATGATG
TTGACGACGATTCAAAACTTGCTTTCGCAAGGGAAAATCTCTCGCCAAACAGCACAGCCTTTCCTGGAAGGAGAGGAATA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098EMP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.708

98.844

0.491
  pilT Vibrio cholerae strain A1552

49.708

98.844

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.385

93.931

0.483
  pilT Pseudomonas stutzeri DSM 10701

48.521

97.688

0.474
  pilT Neisseria meningitidis 8013

48.521

97.688

0.474
  pilT Neisseria gonorrhoeae MS11

48.225

97.688

0.471
  pilT Acinetobacter nosocomialis M2

48.503

96.532

0.468
  pilT Acinetobacter baumannii D1279779

48.204

96.532

0.465
  pilT Acinetobacter baumannii strain A118

48.204

96.532

0.465
  pilT Pseudomonas aeruginosa PAK

47.633

97.688

0.465
  pilT Acinetobacter baylyi ADP1

46.921

98.555

0.462
  pilT Legionella pneumophila strain ERS1305867

46.707

96.532

0.451
  pilT Legionella pneumophila strain Lp02

46.707

96.532

0.451
  pilU Pseudomonas stutzeri DSM 10701

43.807

95.665

0.419
  pilU Vibrio cholerae strain A1552

40.476

97.11

0.393
  pilU Acinetobacter baylyi ADP1

38.623

96.532

0.373

Multiple sequence alignment