Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PFLUOLIPICF7_RS10010 Genome accession   NZ_CP005975
Coordinates   2198983..2200017 (+) Length   344 a.a.
NCBI ID   WP_045791532.1    Uniprot ID   -
Organism   Pseudomonas simiae strain PICF7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2193983..2205017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFLUOLIPICF7_RS09980 (PFLUOLIPICF7_10035) - 2194470..2194904 (-) 435 WP_010207238.1 DUF4426 domain-containing protein -
  PFLUOLIPICF7_RS09985 (PFLUOLIPICF7_10040) metW 2194926..2195546 (-) 621 WP_010207236.1 methionine biosynthesis protein MetW -
  PFLUOLIPICF7_RS09990 (PFLUOLIPICF7_10045) metX 2195554..2196693 (-) 1140 WP_045791531.1 homoserine O-succinyltransferase MetX -
  PFLUOLIPICF7_RS09995 (PFLUOLIPICF7_10050) - 2196901..2197491 (-) 591 WP_010207234.1 YggT family protein -
  PFLUOLIPICF7_RS10000 (PFLUOLIPICF7_10055) proC 2197501..2198319 (-) 819 WP_044288017.1 pyrroline-5-carboxylate reductase -
  PFLUOLIPICF7_RS10005 - 2198354..2198925 (-) 572 Protein_1984 YggS family pyridoxal phosphate-dependent enzyme -
  PFLUOLIPICF7_RS10010 (PFLUOLIPICF7_10060) pilT 2198983..2200017 (+) 1035 WP_045791532.1 type IV pilus twitching motility protein PilT Machinery gene
  PFLUOLIPICF7_RS10015 (PFLUOLIPICF7_10065) - 2200158..2200796 (-) 639 WP_021492670.1 C40 family peptidase -
  PFLUOLIPICF7_RS10020 (PFLUOLIPICF7_10070) - 2201065..2201475 (+) 411 WP_164673138.1 NINE protein -
  PFLUOLIPICF7_RS10025 (PFLUOLIPICF7_10075) - 2201625..2202896 (-) 1272 WP_010207225.1 dihydroorotase -
  PFLUOLIPICF7_RS10030 (PFLUOLIPICF7_10080) - 2202893..2203897 (-) 1005 WP_045791533.1 aspartate carbamoyltransferase catalytic subunit -
  PFLUOLIPICF7_RS10035 (PFLUOLIPICF7_10085) pyrR 2203922..2204428 (-) 507 WP_010207223.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PFLUOLIPICF7_RS10040 (PFLUOLIPICF7_10090) ruvX 2204510..2204947 (-) 438 WP_003195067.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37527.30 Da        Isoelectric Point: 6.7318

>NTDB_id=112726 PFLUOLIPICF7_RS10010 WP_045791532.1 2198983..2200017(+) (pilT) [Pseudomonas simiae strain PICF7]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEVWPLEGPVLSPTQVADLLSPLLNQHQQKDFETSLETDFAFELPDVAR
FRANVFRQDRGLSAVFRTIPSEVRSLESLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDFLNQHRRHHILTLED
PIEFIHTPKMALINQRQVHRDTHGFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KVLVGEGLVSREDAREKARVPTDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=112726 PFLUOLIPICF7_RS10010 WP_045791532.1 2198983..2200017(+) (pilT) [Pseudomonas simiae strain PICF7]
ATGGATATCACTGAACTACTCACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCCGCCGGCCTGGCGCCGAT
GCTGCGGGTTGATGGCGAGGTCTGGCCGCTGGAGGGGCCGGTGCTTTCACCTACGCAAGTAGCGGACTTATTGAGCCCTT
TGCTCAATCAGCATCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCGTTCGAGTTGCCCGACGTGGCGCGT
TTCCGGGCCAATGTGTTCCGGCAGGATCGCGGGCTGAGCGCGGTGTTTCGTACCATACCGTCCGAAGTCCGGAGCTTGGA
AAGCCTTGGTCTCGGTGAAGTATTCCAGCGTATCGCCCAACTGCCCCGGGGCCTCGTGTTGGTCACCGGCCCGACCGGTT
CAGGCAAGTCCACCACCCTGGCGGCGATGATTGATTTTCTCAATCAGCATCGGCGCCATCACATCCTCACCCTTGAAGAC
CCCATCGAATTTATCCACACGCCGAAAATGGCGTTGATCAACCAACGCCAGGTGCATCGCGACACCCATGGCTTTTCGGT
TGCCCTGCGCTCGGCCCTGAGGGAAGACCCGGACGTGATCCTGGTGGGCGAACTGCGTGACTTGGAGACCATCCGCCTGG
CGCTGACGGCGGCCGAGACCGGGCACCTGGTATTTGGCACCCTGCACACCAGTTCGGCCGCAAAGACCGTAGACCGGCTG
GTGGATGTGTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCGATGTTGTCGGAGTCGTTGCAAGCGGTGGTGTCCCAGGT
GCTGGTGAAGAAGATCGGCGGCGGGCGGGTGGCGGCTCACGAGATCATGCTGGGCACGCCGGCCATTCGGAATTTGATCC
GCGAGGACAAGGTGGCGCAGATGGTCTCAGCGATCCAGACGGGTGGGGCGTTGGGGATGAAGACGTTGGATATGAGCTTG
AAGGTGCTGGTCGGGGAGGGGCTGGTCAGCCGGGAAGACGCGCGGGAGAAGGCGAGGGTACCGACAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.837

100

0.738
  pilT Pseudomonas stutzeri DSM 10701

71.512

100

0.715
  pilT Acinetobacter baumannii D1279779

69.795

99.128

0.692
  pilT Acinetobacter baumannii strain A118

69.795

99.128

0.692
  pilT Acinetobacter nosocomialis M2

69.795

99.128

0.692
  pilT Acinetobacter baylyi ADP1

69.208

99.128

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.688

97.965

0.634
  pilT Vibrio cholerae strain A1552

64.688

97.965

0.634
  pilT Legionella pneumophila strain Lp02

63.905

98.256

0.628
  pilT Legionella pneumophila strain ERS1305867

63.905

98.256

0.628
  pilT Neisseria meningitidis 8013

62.059

98.837

0.613
  pilT Neisseria gonorrhoeae MS11

61.765

98.837

0.61
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.494
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

38.551

100

0.387
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381

Multiple sequence alignment