Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   FGL10_RS08335 Genome accession   NZ_LR590477
Coordinates   1546933..1547376 (+) Length   147 a.a.
NCBI ID   WP_003709293.1    Uniprot ID   D0W9X3
Organism   Neisseria lactamica strain NCTC10617     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1541933..1552376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL10_RS08305 (NCTC10617_01446) rpoN 1542287..1543630 (-) 1344 WP_003709281.1 RNA polymerase factor sigma-54 -
  FGL10_RS08310 (NCTC10617_01447) katA 1543978..1545492 (+) 1515 WP_036469787.1 catalase KatA -
  FGL10_RS12115 - 1545568..1545756 (+) 189 WP_003709286.1 hypothetical protein -
  FGL10_RS12120 - 1546061..1546225 (-) 165 WP_155270588.1 hypothetical protein -
  FGL10_RS08335 (NCTC10617_01448) pilE 1546933..1547376 (+) 444 WP_003709293.1 pilin Machinery gene
  FGL10_RS08350 (NCTC10617_01449) - 1547936..1548502 (+) 567 WP_003709298.1 manganese efflux pump MntP family protein -
  FGL10_RS12815 - 1548618..1548749 (-) 132 WP_269321711.1 hypothetical protein -
  FGL10_RS08355 (NCTC10617_01450) - 1548766..1550802 (-) 2037 WP_036469791.1 M3 family metallopeptidase -
  FGL10_RS08360 (NCTC10617_01451) - 1550890..1551762 (-) 873 WP_003709304.1 mechanosensitive ion channel family protein -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15420.68 Da        Isoelectric Point: 8.9788

>NTDB_id=1126942 FGL10_RS08335 WP_003709293.1 1546933..1547376(+) (pilE) [Neisseria lactamica strain NCTC10617]
MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEALTLAEGQKAAVVEYYSDNGTFPTTNASAGIANASTIK
GKYVQKVEVNGDATSATITSTMNASGVNKDISGKTLLLVGKQNSGSFEWTCKAGTVDTKFLPSSCRK

Nucleotide


Download         Length: 444 bp        

>NTDB_id=1126942 FGL10_RS08335 WP_003709293.1 1546933..1547376(+) (pilE) [Neisseria lactamica strain NCTC10617]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTCATCGCCATCGTCGGTATCTTGGCAGCCGTCGC
CCTGCCCGCATACCAAGACTACACCGCGCGCGCCCAAATGTCCGAAGCCCTGACTTTGGCCGAAGGCCAAAAAGCCGCAG
TGGTCGAGTATTACTCCGACAACGGCACGTTCCCGACAACCAATGCTTCCGCAGGTATTGCTAATGCCTCTACGATTAAA
GGTAAATATGTACAAAAAGTGGAAGTTAATGGCGATGCTACCTCTGCCACCATTACCTCTACTATGAATGCCTCAGGCGT
GAACAAAGATATTTCAGGTAAAACCTTGCTACTCGTCGGCAAACAAAACTCCGGCTCGTTCGAATGGACTTGTAAAGCAG
GTACTGTGGACACCAAATTCCTGCCGTCTTCCTGCCGCAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0W9X3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

58.642

100

0.646
  pilE Neisseria gonorrhoeae MS11

57.669

100

0.639
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.508

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

39.881

100

0.456
  pilA/pilA1 Eikenella corrodens VA1

41.772

100

0.449
  pilA2 Legionella pneumophila str. Paris

44.755

97.279

0.435
  pilA2 Legionella pneumophila strain ERS1305867

44.755

97.279

0.435
  comP Acinetobacter baylyi ADP1

40.909

100

0.429
  pilA Acinetobacter nosocomialis M2

44.526

93.197

0.415
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.735

100

0.408
  pilA Pseudomonas aeruginosa PAK

37.179

100

0.395
  pilA Acinetobacter baumannii strain A118

39.041

99.32

0.388
  pilA Vibrio cholerae C6706

36.735

100

0.367
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.735

100

0.367
  pilA Vibrio cholerae strain A1552

36.735

100

0.367
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.054

100

0.361

Multiple sequence alignment