Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OU5_RS19230 Genome accession   NZ_CP005960
Coordinates   4334524..4335558 (+) Length   344 a.a.
NCBI ID   WP_010466841.1    Uniprot ID   A0A024EEB8
Organism   Pseudomonas mandelii JR-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4329524..4340558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU5_RS19195 (OU5_3844) - 4329608..4330042 (-) 435 WP_010466827.1 DUF4426 domain-containing protein -
  OU5_RS19200 (OU5_3845) metW 4330069..4330689 (-) 621 WP_010466828.1 methionine biosynthesis protein MetW -
  OU5_RS19205 (OU5_3846) metX 4330697..4331836 (-) 1140 WP_010466830.1 homoserine O-succinyltransferase MetX -
  OU5_RS19210 (OU5_3847) - 4332019..4332315 (-) 297 WP_010466832.1 DUF167 domain-containing protein -
  OU5_RS19215 (OU5_3848) - 4332315..4332902 (-) 588 WP_010466835.1 YggT family protein -
  OU5_RS19220 (OU5_3849) proC 4332927..4333745 (-) 819 WP_010466837.1 pyrroline-5-carboxylate reductase -
  OU5_RS19225 (OU5_3850) - 4333780..4334466 (-) 687 WP_010466839.1 YggS family pyridoxal phosphate-dependent enzyme -
  OU5_RS19230 (OU5_3851) pilT 4334524..4335558 (+) 1035 WP_010466841.1 type IV pilus twitching motility protein PilT Machinery gene
  OU5_RS19235 (OU5_3852) - 4335710..4336327 (-) 618 WP_042932501.1 C40 family peptidase -
  OU5_RS19240 (OU5_3853) - 4336577..4337008 (+) 432 WP_010466845.1 NINE protein -
  OU5_RS19245 (OU5_3854) - 4337103..4338374 (-) 1272 WP_010466848.1 dihydroorotase -
  OU5_RS19250 (OU5_3855) - 4338371..4339375 (-) 1005 WP_007897943.1 aspartate carbamoyltransferase catalytic subunit -
  OU5_RS19255 (OU5_3856) pyrR 4339403..4339906 (-) 504 WP_010466851.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  OU5_RS19260 (OU5_3857) ruvX 4339987..4340424 (-) 438 WP_005792259.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38114.76 Da        Isoelectric Point: 7.0158

>NTDB_id=112641 OU5_RS19230 WP_010466841.1 4334524..4335558(+) (pilT) [Pseudomonas mandelii JR-1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGEVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=112641 OU5_RS19230 WP_010466841.1 4334524..4335558(+) (pilT) [Pseudomonas mandelii JR-1]
ATGGATATCACTGAGCTGCTGGCCTTCAGCGCTAAACAGGGCGCGTCCGACCTGCACCTGTCTGCCGGCCTGCCACCGAT
GATTCGCGTCGACGGCGACGTGCGGCGCATCAACCTGCCGGCCCTGGACCACAAACAGGTGCATGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACACCTGGAGACTGATTTTTCCTTCGAAGTGCCCGGCGTGGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGGGGGGCGGGTGCGGTATTCCGGACCATCCCGTCGAAAGTCCTGAGCATGGA
CGACCTCGGCATGGGCGAGGTGTTTCGCAAGATCACCGAGGCCCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGTT
CCGGCAAGTCCACCACCCTGGCGGCGATGATCGATTACCTGAACAACCATCGCCATCACCACATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCATGAATCGCGTAAATGCCTGATCAATCAGCGCGAAGTCCACCGTGATACCCGTAGCTTCGCCAC
GGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGACGTGATTCTGGTGGGGGAAATGCGTGACCTGGAGACTATTCGCCTGG
CATTGACCGCCGCCGAGACCGGTCACCTGGTGTTCGGCACGCTGCACACCACGTCAGCGGCGAAGACCATCGACCGGGTG
GTGGACGTGTTTCCGGGGGACGAGAAATCGATGGTGCGGTCGATGCTGTCCGAGTCGTTACTGGCGGTGGTGTCGCAGAC
CCTGATCAAGAAGATCGGCGGTGGGCGCATTGCGGCGCACGAAATCATGCTCGGCACGTCGGCGATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCCTCGATTCAGACCGGGGGCAGCCTGGGGATGCAGACGCTGGACATGTGCTTA
AAGGATTTGGTGACCAAGGGCTTGATCAGCCGGGAGCATGCCCGGGAGAAGGCGCGGACGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024EEB8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

78.779

100

0.788
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment