Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L085_RS07850 Genome accession   NZ_CP005927
Coordinates   1736335..1737357 (+) Length   340 a.a.
NCBI ID   WP_044030494.1    Uniprot ID   -
Organism   Serratia sp. FS14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1731335..1742357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L085_RS24535 - 1731637..1731878 (+) 242 Protein_1541 DUF4160 domain-containing protein -
  L085_RS07815 (L085_07810) - 1731862..1732110 (+) 249 WP_004937422.1 DUF2442 domain-containing protein -
  L085_RS07820 (L085_07815) hemW 1732147..1733289 (-) 1143 WP_044030492.1 radical SAM family heme chaperone HemW -
  L085_RS07825 (L085_07820) - 1733282..1733875 (-) 594 WP_044030493.1 XTP/dITP diphosphatase -
  L085_RS07830 (L085_07825) yggU 1733893..1734183 (-) 291 WP_004948014.1 DUF167 family protein YggU -
  L085_RS07835 (L085_07830) - 1734180..1734734 (-) 555 WP_004937436.1 YggT family protein -
  L085_RS07840 (L085_07835) proC 1734766..1735587 (-) 822 WP_033632092.1 pyrroline-5-carboxylate reductase -
  L085_RS07845 (L085_07840) - 1735607..1736314 (-) 708 WP_004937443.1 YggS family pyridoxal phosphate-dependent enzyme -
  L085_RS07850 (L085_07845) pilT 1736335..1737357 (+) 1023 WP_044030494.1 type IV pilus twitching motility protein PilT Machinery gene
  L085_RS07855 (L085_07850) ruvX 1737354..1737776 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  L085_RS07860 (L085_07855) - 1737776..1738339 (-) 564 WP_044030495.1 YqgE/AlgH family protein -
  L085_RS07865 (L085_07860) gshB 1738473..1739423 (-) 951 WP_033632096.1 glutathione synthase -
  L085_RS07870 (L085_07865) rsmE 1739434..1740165 (-) 732 WP_015379044.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L085_RS07875 (L085_07870) endA 1740235..1740930 (-) 696 WP_044030496.1 deoxyribonuclease I -
  L085_RS07880 (L085_07875) - 1741032..1741544 (-) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36434.79 Da        Isoelectric Point: 8.2075

>NTDB_id=112591 L085_RS07850 WP_044030494.1 1736335..1737357(+) (pilT) [Serratia sp. FS14]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCNGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAALAAPAIVPTLLRRADGLILVTGATGSGKSTTLAAMIDEINRHQPRHVLTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPCGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAGEGTRG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=112591 L085_RS07850 WP_044030494.1 1736335..1737357(+) (pilT) [Serratia sp. FS14]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTTTGCAACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGCGCTGGCGGCGCCGGCCATCGTCCCGACGTTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACGTTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAACTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTTGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAGGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGTGCGCGGGGGAGGGAACGAGGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

49.096

97.647

0.479
  pilT Legionella pneumophila strain Lp02

49.096

97.647

0.479
  pilT Pseudomonas aeruginosa PAK

48.485

97.059

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.182

97.059

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.258

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.628

95

0.376
  pilU Pseudomonas stutzeri DSM 10701

38.509

94.706

0.365

Multiple sequence alignment