Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL296_RS03795 Genome accession   NZ_LR134485
Coordinates   766159..767139 (+) Length   326 a.a.
NCBI ID   WP_124020910.1    Uniprot ID   -
Organism   Citrobacter youngae strain NCTC13709     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 761159..772139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL296_RS03755 (NCTC13709_00765) hemW 761582..762718 (-) 1137 WP_032937402.1 radical SAM family heme chaperone HemW -
  EL296_RS03760 (NCTC13709_00766) - 762711..763304 (-) 594 WP_115642890.1 XTP/dITP diphosphatase -
  EL296_RS03765 (NCTC13709_00767) yggU 763312..763602 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  EL296_RS03770 (NCTC13709_00768) - 763599..764165 (-) 567 WP_003825417.1 YggT family protein -
  EL296_RS03775 (NCTC13709_00769) - 764183..764887 (-) 705 WP_124020912.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL296_RS03780 (NCTC13709_00770) - 764905..765030 (+) 126 Protein_727 type IV pili twitching motility protein PilT -
  EL296_RS24305 (NCTC13709_00771) - 765547..765780 (+) 234 WP_232034673.1 hypothetical protein -
  EL296_RS03790 - 765971..766141 (-) 171 Protein_729 YggS family pyridoxal phosphate enzyme -
  EL296_RS03795 (NCTC13709_00772) pilT 766159..767139 (+) 981 WP_124020910.1 type IV pilus twitching motility protein PilT Machinery gene
  EL296_RS03800 (NCTC13709_00773) ruvX 767136..767552 (-) 417 WP_006686843.1 Holliday junction resolvase RuvX -
  EL296_RS03805 (NCTC13709_00774) - 767552..768115 (-) 564 WP_048213499.1 YqgE/AlgH family protein -
  EL296_RS03810 (NCTC13709_00775) gshB 768227..769174 (-) 948 WP_003027083.1 glutathione synthase -
  EL296_RS03815 (NCTC13709_00776) rsmE 769187..769918 (-) 732 WP_115601492.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EL296_RS03820 (NCTC13709_00777) endA 769993..770700 (-) 708 WP_016150969.1 deoxyribonuclease I -
  EL296_RS03825 (NCTC13709_00778) - 770795..771292 (-) 498 WP_061068543.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36093.12 Da        Isoelectric Point: 6.3398

>NTDB_id=1123139 EL296_RS03795 WP_124020910.1 766159..767139(+) (pilT) [Citrobacter youngae strain NCTC13709]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRRGKIENAPFTTPDVENLLMCWLSEQQQVQWREQGQVDFAVTLTDSRRLRA
SAFAHQQGTSLALRLLPLDCPHLDDLQTPEAIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYASQRCLIQQREVGVHCVSFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAASEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTLAVGNLIREGKNHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1123139 EL296_RS03795 WP_124020910.1 766159..767139(+) (pilT) [Citrobacter youngae strain NCTC13709]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCAGGCGTGGAAAAATCGAAAACGCGCCATTTACCACGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAGCAAGGGCAGGTTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGCCCATCAGCAAGGAACGTCACTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTCCA
GACTCCCGAGGCCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCCACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGACACATTCTGACGCTGGAAGATCCTATTGAA
TACCGCTACGCCAGCCAGCGTTGTCTCATTCAACAGCGGGAGGTGGGCGTACACTGCGTCTCTTTCGCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGCTACACACGCGTGGGGCTGCACAAGCCATTGCGCGGCTGGTGGATTCC
TTTGCTGCATCAGAGAAAGATCCGGTGCGTAATCAACTGGCAGACAGCTTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAGCTGCTCGTTAACACGCTCGCCGTGGGCAATTTGATCCGCGAAGGGA
AGAACCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365

Multiple sequence alignment