Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL330_RS01415 Genome accession   NZ_LR134482
Coordinates   297340..298374 (+) Length   344 a.a.
NCBI ID   WP_003303153.1    Uniprot ID   A0A1I3QZE2
Organism   Stutzerimonas stutzeri strain NCTC10475     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 292340..303374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL330_RS01395 (NCTC10475_00280) - 292871..294838 (-) 1968 WP_101275340.1 dynamin-like GTPase family protein -
  EL330_RS01400 (NCTC10475_00281) - 295110..295700 (-) 591 WP_041015113.1 YggT family protein -
  EL330_RS01405 (NCTC10475_00282) proC 295751..296575 (-) 825 WP_101275339.1 pyrroline-5-carboxylate reductase -
  EL330_RS01410 (NCTC10475_00283) - 296586..297275 (-) 690 WP_101275338.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL330_RS01415 (NCTC10475_00284) pilT 297340..298374 (+) 1035 WP_003303153.1 type IV pilus twitching motility protein PilT Machinery gene
  EL330_RS01420 (NCTC10475_00285) - 298486..298899 (+) 414 Protein_282 type IV pili twitching motility protein PilT -
  EL330_RS01425 (NCTC10475_00286) - 298896..299732 (-) 837 WP_101275337.1 TIGR03915 family putative DNA repair protein -
  EL330_RS01430 (NCTC10475_00287) - 299732..300943 (-) 1212 WP_041015122.1 putative DNA modification/repair radical SAM protein -
  EL330_RS01435 (NCTC10475_00288) - 301077..301472 (+) 396 WP_003282737.1 NINE protein -
  EL330_RS01440 (NCTC10475_00289) - 301550..302824 (-) 1275 WP_023446074.1 dihydroorotase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37946.67 Da        Isoelectric Point: 6.8360

>NTDB_id=1123051 EL330_RS01415 WP_003303153.1 297340..298374(+) (pilT) [Stutzerimonas stutzeri strain NCTC10475]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1123051 EL330_RS01415 WP_003303153.1 297340..298374(+) (pilT) [Stutzerimonas stutzeri strain NCTC10475]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGATTTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGAATCAACTTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTGGAGACCGACTTCTCGTTCGAAGTGCCGGGCGTGGCGCGT
TTTCGCGTCAACGCGTTCAACCAGAATCGCGGTTCCGGCGCGGTGTTCCGGACCATTCCTTCCAAGGTGCTGACCATGGA
AGACCTGGGGATGGGCGAGGTTTTCCGCAAGATCACCGATGTGCCGCGCGGGCTGGTACTGGTCACCGGACCGACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTTGATTACCTGAACAACACCAAGTACCACCACATCCTTACCATCGAGGAC
CCCATCGAATTCGTTCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTCCATCGTGACACCCTGGGCTTCTCCGA
GGCACTGCGTTCGGCACTGCGTGAAGACCCGGACATCATTCTCGTTGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGTCACTTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAAAAGTCCATGGTCCGTTCGATGCTCTCCGAATCGCTGCAGGCGGTTATCTCGCAGAC
CCTGCTGAAGAAGGTTGGCGGCGGCCGTGTCGCGGCCCACGAGATCATGATCGGTACGCCGGCGATCCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACTCGTGCCTC
AAGGGGCTGCTCGCCAAAGGGCTGATCTCGCGAGACAGCGCCAAGGAAAAGGCCAAGCAGCCAGAGAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I3QZE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.419

100

0.994
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment