Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL201_RS11660 Genome accession   NZ_LR134380
Coordinates   2610448..2611569 (-) Length   373 a.a.
NCBI ID   WP_027222418.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. pascullei strain NCTC12273     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2605448..2616569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL201_RS11645 (NCTC12273_02334) - 2606327..2606773 (-) 447 WP_027222415.1 hypothetical protein -
  EL201_RS11650 (NCTC12273_02335) - 2607050..2608345 (+) 1296 WP_027222416.1 serine/threonine transporter -
  EL201_RS11655 (NCTC12273_02336) ankK 2608436..2610361 (-) 1926 WP_027222417.1 Dot/Icm T4SS effector AnkK/LegA5 -
  EL201_RS11660 (NCTC12273_02337) pilU 2610448..2611569 (-) 1122 WP_027222418.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL201_RS11665 (NCTC12273_02338) - 2611659..2613587 (-) 1929 WP_027222419.1 FAD-binding oxidoreductase -
  EL201_RS11670 (NCTC12273_02339) - 2613612..2614442 (-) 831 WP_027222420.1 phytanoyl-CoA dioxygenase family protein -
  EL201_RS11675 (NCTC12273_02340) radA 2614439..2615794 (-) 1356 WP_027222421.1 DNA repair protein RadA -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41715.00 Da        Isoelectric Point: 5.4724

>NTDB_id=1122514 EL201_RS11660 WP_027222418.1 2610448..2611569(-) (pilU) [Legionella pneumophila subsp. pascullei strain NCTC12273]
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGVTSPVGQVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGIDNQRVAAVEILINSPYISDLIEKGKIDDIKEVMARSTEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKNFNVQDENLQIQPDDLDKI

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1122514 EL201_RS11660 WP_027222418.1 2610448..2611569(-) (pilU) [Legionella pneumophila subsp. pascullei strain NCTC12273]
ATGGATATTACTCCTTTTTTTAAGCTCATGGTGGATAAAGGTGCCTCAGATTTGTTTTTCAGTGTCGGGGCGCCGCCGAA
TATTAAAATCGAAGGAGTCACGTCACCTGTTGGCCAGGTACCTATCAAATCGAATCAAATGGCAGAAATTGTTTTATCTA
TTATGAATGACGCACAGCAAAAAGAATTTGAGTCGAAAATGGAGCTCAATATGGCTTTTTCGTTAGCTGGGGTAGGACGT
TTCCGCATCAATTTATTCAGGCAACGTGGTGAAGTGGCTATGGTAGTGCGTTACATCAAGGGCGAAATACCTTCTATAGA
AGATTTAAATCTTCCCCAAATTTTAAAGTCTTTGGTTTTGGAGTTACGAGGTTTAGTATTAGTTGTTGGATCTACGGGCT
CAGGTAAATCAACTACTCTGGCCGCAATGATCAACCACCGCAATGAAAATCACAGGGGGCACATTTTAACAATAGAGGAT
CCTATTGAATATATACATCAGCATAAAAAATCAATAGTTGATCAACGTGAGATAGGTATTGACACTCTAAGCTATGACAA
TGCGTTGAAAAATGCAATGAGAGAAGCACCTGATGTGATATTAATTGGTGAAATTCGTGATCTTAACTCAATGAAACACG
CCATAGCCTATGCTGAAACAGGACATTTGTGCATTTCTACTTTGCATGCCAATAACGCCAATCAGGCGCTGGATAGAGTG
ATTAATTTTTTCCCTGATGAGGCAAGACAACAAATATTGTTAGATTTATCTCTTAATTTAAAAGCAATCATTTCATTACG
TTTGATACCAGGAATAGATAATCAGCGTGTCGCAGCGGTGGAGATCTTGATTAACTCTCCATATATTTCTGATTTGATTG
AAAAAGGGAAGATAGACGATATTAAAGAAGTTATGGCGAGAAGTACGGAACAAGGAATGCAGACTTTCGATCAGGCTTTG
TTTAATTTATATAAAGAAGGAAAAATTACCAAAGAGAATGCGATTAAGTACGCTGATTCAAAAAATAATGTGGGTTTACA
AATTCGCTTGGGTGAAGAAAAAAACTTTAATGTCCAAGATGAGAACCTACAAATACAACCCGATGATTTGGATAAAATTT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(40-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.337

95.442

0.528
  pilU Acinetobacter baylyi ADP1

53.221

95.71

0.509
  pilU Vibrio cholerae strain A1552

48.286

93.834

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.428

90.885

0.413
  pilT Legionella pneumophila strain Lp02

42.982

91.689

0.394
  pilT Legionella pneumophila strain ERS1305867

42.982

91.689

0.394
  pilT Pseudomonas aeruginosa PAK

42.566

91.957

0.391
  pilT Neisseria meningitidis 8013

42.075

93.029

0.391
  pilT Neisseria gonorrhoeae MS11

42.075

93.029

0.391
  pilT Acinetobacter baumannii D1279779

42.814

89.544

0.383
  pilT Acinetobacter baumannii strain A118

42.814

89.544

0.383
  pilT Acinetobacter nosocomialis M2

42.814

89.544

0.383
  pilT Pseudomonas stutzeri DSM 10701

41.399

91.957

0.381
  pilT Acinetobacter baylyi ADP1

40.588

91.153

0.37
  pilT Vibrio cholerae strain A1552

41.641

88.204

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.641

88.204

0.367

Multiple sequence alignment