Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL199_RS10230 Genome accession   NZ_LR134365
Coordinates   2102201..2103262 (-) Length   353 a.a.
NCBI ID   WP_004141679.1    Uniprot ID   C8NB69
Organism   Cardiobacterium hominis strain NCTC10426     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2097201..2108262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL199_RS10215 (NCTC10426_01992) - 2098356..2098643 (-) 288 WP_004141682.1 helix-turn-helix domain-containing protein -
  EL199_RS10220 (NCTC10426_01993) parE 2098800..2100719 (-) 1920 WP_040355505.1 DNA topoisomerase IV subunit B -
  EL199_RS10225 (NCTC10426_01994) - 2100887..2102185 (-) 1299 WP_004141680.1 PilT/PilU family type 4a pilus ATPase -
  EL199_RS10230 (NCTC10426_01995) pilT 2102201..2103262 (-) 1062 WP_004141679.1 type IV pilus twitching motility protein PilT Machinery gene
  EL199_RS10235 (NCTC10426_01996) proC 2103413..2104255 (+) 843 WP_004141675.1 pyrroline-5-carboxylate reductase -
  EL199_RS10240 (NCTC10426_01997) - 2104294..2104809 (+) 516 WP_004141674.1 YggT family protein -
  EL199_RS10245 (NCTC10426_01998) - 2104813..2105106 (+) 294 WP_004141673.1 DUF167 family protein -
  EL199_RS10250 (NCTC10426_01999) mnmG 2105103..2106980 (+) 1878 WP_004141672.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  EL199_RS13355 (NCTC10426_02000) - 2106977..2107489 (+) 513 WP_232008294.1 hypothetical protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39562.39 Da        Isoelectric Point: 6.9782

>NTDB_id=1122323 EL199_RS10230 WP_004141679.1 2102201..2103262(-) (pilT) [Cardiobacterium hominis strain NCTC10426]
MSDKHETKLDDLLRFTDKQKASDLHLSSELPPMIRVDGDIKRLNLPPLDRQQLRDMIYSIMTDTQVKNFEEHWEADFSTE
VKGIARFRVNVFNQNRGLAVVFRTIPSRVLSLEDLKAPEKFVDIIDVPRGLVLVTGPTGSGKSTTLAAMLNHINENRHEH
ILTIEDPIEFVHESKKCLVNQREVHRDTQSFSNALRAALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRIIDVFPGEEKQLVRSMLSESLRAVIAQTLLKKIAGGRIAAHEILVATSAVKNLIREDKIAQIYSSIQTGAQYGMQ
TLDQCLQKLVSQGLIDRQTAASKAHDKTLFLHG

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=1122323 EL199_RS10230 WP_004141679.1 2102201..2103262(-) (pilT) [Cardiobacterium hominis strain NCTC10426]
ATGAGCGATAAGCACGAAACCAAACTGGACGACCTGCTGCGGTTTACTGACAAGCAGAAGGCGTCGGACTTGCACTTGAG
TTCTGAGTTGCCGCCGATGATTCGCGTGGACGGCGATATCAAGCGTCTCAACCTGCCGCCGCTGGACCGCCAGCAACTGC
GCGACATGATTTATTCCATCATGACCGATACGCAGGTCAAGAACTTCGAGGAGCACTGGGAGGCGGACTTCTCCACCGAA
GTCAAGGGTATCGCCCGTTTCCGGGTAAACGTTTTTAACCAGAACCGCGGTCTGGCGGTGGTGTTCCGGACGATTCCGAG
CCGCGTGCTGTCGCTTGAGGATCTGAAAGCGCCGGAAAAATTCGTCGATATTATTGACGTGCCGCGTGGCCTCGTATTGG
TTACCGGCCCGACCGGTTCCGGCAAGTCCACCACGCTGGCGGCGATGCTAAACCACATCAACGAAAACCGCCACGAACAC
ATCCTTACCATCGAAGATCCGATTGAATTCGTGCACGAATCGAAAAAATGCCTGGTGAACCAGCGCGAGGTACACCGCGA
CACGCAAAGTTTCTCCAACGCCCTGCGCGCCGCGCTGCGTGAAGACCCGGACATCATTCTCGTTGGTGAGTTGCGCGACC
TGGAAACCATCCGCCTTGCGCTGACCGCCGCCGAAACCGGTCACTTGGTGTTTGGCACCCTGCACACCTCGTCTGCGGCA
AAAACCATTGACCGTATCATCGATGTCTTCCCCGGCGAAGAAAAACAACTGGTACGCTCCATGCTCTCCGAATCACTGCG
GGCAGTTATCGCGCAAACGCTTTTGAAGAAAATCGCCGGCGGCCGTATCGCTGCGCACGAAATCCTCGTCGCCACTTCAG
CGGTGAAAAACCTCATCCGCGAAGACAAAATCGCGCAAATCTATTCCTCCATCCAAACCGGTGCGCAATACGGCATGCAA
ACCCTTGACCAATGCCTGCAAAAACTGGTGTCACAAGGCCTCATCGACCGGCAAACCGCCGCCTCGAAAGCGCACGACAA
AACCCTCTTCCTGCACGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(18-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C8NB69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

73.214

95.184

0.697
  pilT Pseudomonas aeruginosa PAK

72.321

95.184

0.688
  pilT Acinetobacter nosocomialis M2

68.513

97.167

0.666
  pilT Acinetobacter baumannii D1279779

68.222

97.167

0.663
  pilT Acinetobacter baumannii strain A118

68.222

97.167

0.663
  pilT Acinetobacter baylyi ADP1

68.129

96.884

0.66
  pilT Legionella pneumophila strain Lp02

68.235

96.317

0.657
  pilT Legionella pneumophila strain ERS1305867

68.235

96.317

0.657
  pilT Neisseria meningitidis 8013

65.588

96.317

0.632
  pilT Neisseria gonorrhoeae MS11

65.294

96.317

0.629
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.565

94.334

0.609
  pilT Vibrio cholerae strain A1552

64.565

94.334

0.609
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.729

97.167

0.493
  pilU Vibrio cholerae strain A1552

41.667

95.184

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.12

94.618

0.38
  pilU Acinetobacter baylyi ADP1

40.312

90.652

0.365

Multiple sequence alignment