Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EL233_RS15870 Genome accession   NZ_LR134319
Coordinates   3400476..3400907 (+) Length   143 a.a.
NCBI ID   WP_126526820.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain NCTC10450     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3395476..3405907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL233_RS15850 (NCTC10450_03193) coaE 3395620..3396228 (-) 609 WP_014595960.1 dephospho-CoA kinase -
  EL233_RS15855 (NCTC10450_03194) pilD 3396360..3397229 (-) 870 WP_126526817.1 prepilin peptidase Machinery gene
  EL233_RS15860 (NCTC10450_03195) pilC 3397232..3398449 (-) 1218 WP_126526818.1 type II secretion system F family protein Machinery gene
  EL233_RS15865 (NCTC10450_03196) pilB 3398452..3400155 (-) 1704 WP_126526819.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL233_RS15870 (NCTC10450_03197) pilE 3400476..3400907 (+) 432 WP_126526820.1 pilin Machinery gene
  EL233_RS15875 (NCTC10450_03198) cysN 3401203..3403101 (-) 1899 WP_126526821.1 sulfate adenylyltransferase subunit CysN -
  EL233_RS15880 (NCTC10450_03199) cysD 3403113..3404030 (-) 918 WP_011912238.1 sulfate adenylyltransferase subunit CysD -
  EL233_RS15885 (NCTC10450_03200) - 3404219..3404977 (-) 759 WP_125872858.1 Nif3-like dinuclear metal center hexameric protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14814.02 Da        Isoelectric Point: 8.4526

>NTDB_id=1121364 EL233_RS15870 WP_126526820.1 3400476..3400907(+) (pilE) [Stutzerimonas stutzeri strain NCTC10450]
MKSQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARAQSTEALNLAGGLKTQVAEFYADKGAWPAITDLGYAAATDVTGK
YVASVNVDSTTGAVTATLKATGVSKDLQGKTVVLTPTAATGKPITWACSTDATNKNVVPTTCR

Nucleotide


Download         Length: 432 bp        

>NTDB_id=1121364 EL233_RS15870 WP_126526820.1 3400476..3400907(+) (pilE) [Stutzerimonas stutzeri strain NCTC10450]
ATGAAATCACAAAAAGGCTTTACCCTTATCGAACTGATGATCGTCGTTGCGATCATTGGCATTCTGGCAGCGATCGCCAT
CCCGCAGTACCAAGACTATACGGCCCGCGCTCAGTCGACAGAGGCGCTGAACTTAGCAGGAGGTCTTAAAACCCAAGTAG
CAGAATTCTATGCGGACAAAGGTGCCTGGCCCGCTATTACTGACCTTGGCTACGCCGCTGCCACTGATGTAACTGGCAAA
TATGTCGCATCGGTGAACGTGGACAGCACTACCGGAGCAGTGACAGCTACTCTTAAGGCGACGGGCGTATCTAAAGATCT
ACAAGGGAAAACAGTAGTTCTCACCCCAACCGCAGCAACCGGCAAACCAATTACTTGGGCCTGCAGCACCGACGCAACCA
ATAAGAATGTCGTACCGACTACCTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

44.937

100

0.497
  pilA/pilA1 Eikenella corrodens VA1

46.104

100

0.497
  pilE Neisseria gonorrhoeae strain FA1090

45.223

100

0.497
  comP Acinetobacter baylyi ADP1

44.371

100

0.469
  pilA2 Legionella pneumophila strain ERS1305867

44.218

100

0.455
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40

100

0.448
  pilA2 Legionella pneumophila str. Paris

45

97.902

0.441
  pilA Pseudomonas aeruginosa PAK

41.447

100

0.441
  pilA Ralstonia pseudosolanacearum GMI1000

44.853

95.105

0.427
  pilA Acinetobacter baumannii strain A118

40.541

100

0.42
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.846

90.909

0.399
  pilA Acinetobacter nosocomialis M2

48.696

80.42

0.392
  pilA Vibrio parahaemolyticus RIMD 2210633

40

90.909

0.364

Multiple sequence alignment