Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL233_RS15865 Genome accession   NZ_LR134319
Coordinates   3398452..3400155 (-) Length   567 a.a.
NCBI ID   WP_126526819.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain NCTC10450     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3393452..3405155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL233_RS15835 (NCTC10450_03190) - 3393636..3394676 (+) 1041 WP_013981947.1 NADP(H)-dependent aldo-keto reductase -
  EL233_RS15840 (NCTC10450_03191) - 3394733..3395413 (+) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  EL233_RS15845 (NCTC10450_03192) yacG 3395426..3395623 (-) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  EL233_RS15850 (NCTC10450_03193) coaE 3395620..3396228 (-) 609 WP_014595960.1 dephospho-CoA kinase -
  EL233_RS15855 (NCTC10450_03194) pilD 3396360..3397229 (-) 870 WP_126526817.1 prepilin peptidase Machinery gene
  EL233_RS15860 (NCTC10450_03195) pilC 3397232..3398449 (-) 1218 WP_126526818.1 type II secretion system F family protein Machinery gene
  EL233_RS15865 (NCTC10450_03196) pilB 3398452..3400155 (-) 1704 WP_126526819.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL233_RS15870 (NCTC10450_03197) pilE 3400476..3400907 (+) 432 WP_126526820.1 pilin Machinery gene
  EL233_RS15875 (NCTC10450_03198) cysN 3401203..3403101 (-) 1899 WP_126526821.1 sulfate adenylyltransferase subunit CysN -
  EL233_RS15880 (NCTC10450_03199) cysD 3403113..3404030 (-) 918 WP_011912238.1 sulfate adenylyltransferase subunit CysD -
  EL233_RS15885 (NCTC10450_03200) - 3404219..3404977 (-) 759 WP_125872858.1 Nif3-like dinuclear metal center hexameric protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62068.01 Da        Isoelectric Point: 5.4682

>NTDB_id=1121363 EL233_RS15865 WP_126526819.1 3398452..3400155(-) (pilB) [Stutzerimonas stutzeri strain NCTC10450]
MSDNISLSGLARQMVLAGVLDERTAQQAQVQALRNKSSFVTYVVQNKLVKGRTVAELASEQFGVALLDLAALDKDVLPKD
TVNEKLARQHRVLPLAKRGNKLYLAVSDPANHQAISDIQFSTGLTTEAVLVEDDKLGDAIDKLFDNGSAGLEDLGDVDLD
GVDVETVSDDIKGDDGGGEADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEVDVPRDVLLREGFP
EQKLGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPSLQRIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1121363 EL233_RS15865 WP_126526819.1 3398452..3400155(-) (pilB) [Stutzerimonas stutzeri strain NCTC10450]
ATGAGCGACAATATTTCACTTTCCGGCTTGGCACGTCAGATGGTCCTGGCTGGTGTATTGGATGAGCGTACTGCACAGCA
GGCGCAGGTGCAGGCGCTGCGAAACAAATCGTCCTTTGTTACTTACGTCGTCCAGAACAAGCTCGTAAAGGGGAGGACCG
TTGCGGAGCTTGCGTCCGAGCAGTTTGGCGTTGCGCTGCTGGATCTGGCTGCTTTGGACAAGGATGTGCTGCCTAAGGAT
ACTGTCAACGAGAAGCTGGCTCGTCAGCATCGGGTGCTCCCGCTGGCGAAGCGTGGCAATAAGCTCTACCTGGCCGTTTC
CGACCCGGCTAACCATCAGGCGATTTCCGATATCCAGTTCAGTACGGGGTTGACTACTGAGGCCGTCCTTGTCGAGGACG
ACAAGCTTGGGGACGCTATCGATAAGTTATTCGATAATGGTTCGGCCGGCCTGGAGGATCTTGGCGATGTTGACCTCGAC
GGGGTAGATGTCGAGACCGTTTCGGATGACATAAAAGGGGATGATGGCGGTGGGGAGGCTGACGATGCGCCGGTTGTGCG
TTTCGTCAACAAGATGTTGCTTGATGCGATCCGGGGCGGCTCATCTGACCTTCATTTTGAGCCTTATGAAAAAGCCTATC
GGGTACGGTTACGTACTGATGGCATTCTGCATGAAGTGGCACGTCCGCCGATTCAACTGGCGCCTCGGATTTCCGCGCGA
CTCAAGGTCATGGCAGGCCTGGATATTTCCGAGCGGCGCAAGCCGCAGGATGGGCGGATCAAAATGAAGCTCTCCAAGAG
CAAGGCCATCGATTTCCGGGTCAACACGCTACCGACCTTGTGGGGCGAAAAGATTGTAATGCGGATTCTTGACCCCTCAA
GTGCTCAAATGGGCATTGATGCCCTGGGATATGAGGAGGATCAGAAAGAGCTGTATATGAATGCTCTGCGGCAACCGCAG
GGTATGATTCTCGTGACTGGTCCGACGGGCTCCGGTAAGACGGTATCCCTCTACACCGGGTTGAACATCCTCAACACCGT
CGATGTGAACATTTCGACGGCGGAGGATCCGGTAGAGATCAACCTCGAGGGTATCAACCAGGTCAACGTCAATCCTAAAC
AGGGCATGGACTTTTCTCAGGCCCTCCGTGCCTTTCTACGTCAGGACCCGGACATCATCATGGTAGGGGAGATCCGCGAT
CTGGAGACCGCCGAGATTGCGATCAAGGCTGCGCAGACGGGCCATATGGTGATGTCGACACTTCATACCAATAGCGCTGC
GGAAACCCTGACGCGTCTGCGCAACATGGGGGTGCCTGCGTTCAACATCGCGACCTCCGTGAACCTGATCATTGCTCAGC
GTCTGGCACGAAAGCTCTGCAATAGCTGCAAGAAAGAGGTTGATGTCCCCCGTGACGTTTTGCTGCGCGAAGGGTTCCCT
GAGCAGAAGCTTGGAACCTTCAAGATTTATGGTCCGGTAGGTTGCGACAACTGCAAGGGCGGCTACAAGGGGCGCGTCGG
TATTTATGAAGTGGTTAAAAACACGCCATCGTTGCAGCGGATTATCATGGAAGACGGAAACTCCATCGATATTTCAACCC
AGATGCGCAAAGACGGCTTCAACGACCTGCGGACCTCGGCTTTGCTGAAAGCCATGCAGGGCGTGACCAGCCTCGAAGAA
GTCAACCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.638

99.471

0.603
  pilB Acinetobacter baylyi ADP1

59.752

99.471

0.594
  pilB Legionella pneumophila strain ERS1305867

55.944

100

0.564
  pilB Vibrio cholerae strain A1552

51.654

95.944

0.496
  pilB Vibrio campbellii strain DS40M4

49.027

99.647

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

52.692

91.711

0.483
  pilF Neisseria gonorrhoeae MS11

51.336

92.416

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

37.838

97.884

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.658

92.945

0.369

Multiple sequence alignment