Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL257_RS25715 Genome accession   NZ_LR134318
Coordinates   5615339..5616373 (-) Length   344 a.a.
NCBI ID   WP_126367315.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain NCTC9428     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5610339..5621373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL257_RS25685 (NCTC9428_05118) ruvX 5610541..5610978 (+) 438 WP_016772738.1 Holliday junction resolvase RuvX -
  EL257_RS25690 (NCTC9428_05119) pyrR 5611060..5611566 (+) 507 WP_126367309.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  EL257_RS25695 (NCTC9428_05120) - 5611594..5612598 (+) 1005 WP_016772736.1 aspartate carbamoyltransferase catalytic subunit -
  EL257_RS25700 (NCTC9428_05121) - 5612595..5613866 (+) 1272 WP_126367311.1 dihydroorotase -
  EL257_RS25705 (NCTC9428_05122) - 5613960..5614391 (-) 432 WP_126367313.1 NINE protein -
  EL257_RS25710 (NCTC9428_05123) - 5614639..5615256 (+) 618 WP_016772733.1 C40 family peptidase -
  EL257_RS25715 (NCTC9428_05124) pilT 5615339..5616373 (-) 1035 WP_126367315.1 type IV pilus twitching motility protein PilT Machinery gene
  EL257_RS25720 (NCTC9428_05125) - 5616430..5617116 (+) 687 WP_126367317.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL257_RS25725 (NCTC9428_05126) proC 5617140..5617958 (+) 819 WP_126367319.1 pyrroline-5-carboxylate reductase -
  EL257_RS25730 (NCTC9428_05127) - 5617970..5618560 (+) 591 WP_126367321.1 YggT family protein -
  EL257_RS25735 (NCTC9428_05128) metX 5618864..5620003 (+) 1140 WP_126367323.1 homoserine O-succinyltransferase MetX -
  EL257_RS25740 (NCTC9428_05129) metW 5620011..5620631 (+) 621 WP_126367325.1 methionine biosynthesis protein MetW -
  EL257_RS25745 (NCTC9428_05130) - 5620658..5621092 (+) 435 WP_126367327.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38020.64 Da        Isoelectric Point: 6.9776

>NTDB_id=1121336 EL257_RS25715 WP_126367315.1 5615339..5616373(-) (pilT) [Pseudomonas fluorescens strain NCTC9428]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHDLIYDIMNDTQRVDFEKNLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAVRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1121336 EL257_RS25715 WP_126367315.1 5615339..5616373(-) (pilT) [Pseudomonas fluorescens strain NCTC9428]
ATGGATATCACTGAATTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGGCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGACGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTACACGATTTGATCTACGACA
TCATGAACGACACCCAACGGGTGGATTTCGAGAAAAATCTGGAAACCGATTTTTCATTCGACGTGCCGGGTGTGGCGCGT
TTTCGGGTCAATGCCTTCAACCAGAATCGCGGCGCCGGTGCGGTGTTCCGCACGATTCCATCGAAAGTGCTGACCATGGA
AGATCTGGCCATGGGCGACGTGTTCCGCAAGATCACCGATGCGCCGCGCGGACTGGTATTGGTCACCGGGCCGACCGGTT
CCGGCAAGTCGACGACGCTGGCGGCAATGATCGACTACCTCAACACCCACCGCCATCATCATATTCTGACGATTGAAGAC
CCGATCGAATTTGTCCACGAATCACGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGTTTCGCCAC
GGCATTGCGCTCGGCACTGCGTGAAGATCCCGACGTGATCCTGGTGGGCGAAATGCGTGATCTGGAAACCATTCGCCTGG
CGCTGACAGCGGCGGAAACCGGGCATCTGGTGTTCGGCACGCTGCACACCACTTCAGCAGCGAAAACCATCGATCGCGTG
GTAGATGTGTTTCCCGGCGATGAGAAATCCATGGTCCGTTCGATGCTGTCGGAATCGTTGCTGGCGGTGGTTTCGCAGAC
TTTGATCAAGAAGATCGGCGGCGGGCGAGTGGCGGCGCACGAAATCATGCTGGGCACCTCGGCGGTTCGCAATTTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACGGGTGGGTCGCTGGGCATGCAGACACTTGATATGTGCCTG
AAGGATCTGGTGACCAAAGGTTTGATCAGCCGCGAGCATGCGCGGGAGAAGGCGCGCACCCCGGATAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

71.598

98.256

0.703
  pilT Legionella pneumophila strain ERS1305867

71.598

98.256

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment