Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL200_RS14115 Genome accession   NZ_LR134301
Coordinates   2993272..2994399 (+) Length   375 a.a.
NCBI ID   WP_057500397.1    Uniprot ID   A0AAI9FXX7
Organism   Stenotrophomonas maltophilia strain NCTC13014     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2988272..2999399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL200_RS14090 (NCTC13014_02799) - 2988364..2989548 (-) 1185 WP_057500395.1 cystathionine gamma-synthase -
  EL200_RS14095 (NCTC13014_02800) - 2989672..2991042 (-) 1371 WP_057500396.1 pyridoxal-phosphate dependent enzyme -
  EL200_RS14100 (NCTC13014_02801) - 2991343..2991579 (-) 237 WP_005407964.1 YdcH family protein -
  EL200_RS14105 (NCTC13014_02803) - 2991904..2992788 (-) 885 WP_080392877.1 OmpA family protein -
  EL200_RS14110 (NCTC13014_02804) - 2992785..2993150 (-) 366 WP_005415160.1 DUF4398 domain-containing protein -
  EL200_RS14115 (NCTC13014_02805) pilU 2993272..2994399 (+) 1128 WP_057500397.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL200_RS14120 (NCTC13014_02806) - 2994530..2995813 (+) 1284 WP_057500398.1 putative DNA modification/repair radical SAM protein -
  EL200_RS14125 (NCTC13014_02807) - 2995794..2997254 (+) 1461 WP_057500399.1 UdgX family uracil-DNA binding protein -
  EL200_RS14130 (NCTC13014_02808) maiA 2997302..2997970 (-) 669 WP_057500405.1 maleylacetoacetate isomerase -
  EL200_RS14135 (NCTC13014_02809) - 2997985..2998974 (-) 990 WP_057500400.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41410.75 Da        Isoelectric Point: 6.3741

>NTDB_id=1120733 EL200_RS14115 WP_057500397.1 2993272..2994399(+) (pilU) [Stenotrophomonas maltophilia strain NCTC13014]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPEAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGVIDQEEALRAADSRDGLALKFRLSEGSTGEHDPYADFSAAAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1120733 EL200_RS14115 WP_057500397.1 2993272..2994399(+) (pilU) [Stenotrophomonas maltophilia strain NCTC13014]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCCTGACCACCGGGGCACCGGTCTA
TATCAAGATCGAGGGGAAGCTTTATCCGCTGGGCAACACCGGCCTGCCGCCCGGCATGGTCAAGAAAATCGCCTACTCGT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGAAGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAACTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAATCGACGTCGCTGGCATCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCTACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCACTGAACCTGCGCGCGGTGATCAGCCAGCG
TCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGGCTCGCTGGAAGAAGGCATGGAGAGTTTCGACCAGTGTCTG
TTCCGGCTGGCCAAGGCCGGCGTCATCGATCAGGAGGAAGCACTGCGTGCGGCCGACTCGCGCGATGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCAGCACTGGCGAGCACGACCCGTACGCGGACTTCTCTGCGGCCGCGCCCAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.718

94.4

0.469
  pilU Vibrio cholerae strain A1552

48.87

94.4

0.461
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baumannii strain A118

42.012

90.133

0.379
  pilT Acinetobacter nosocomialis M2

42.012

90.133

0.379
  pilT Acinetobacter baumannii D1279779

42.012

90.133

0.379
  pilT Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Vibrio cholerae strain A1552

41.441

88.8

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

88.8

0.368
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363

Multiple sequence alignment