Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL098_RS02910 Genome accession   NZ_LR134201
Coordinates   569729..570754 (+) Length   341 a.a.
NCBI ID   WP_126354610.1    Uniprot ID   A0A3S4MBM3
Organism   Cedecea lapagei strain NCTC11466     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 564729..575754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL098_RS02875 (NCTC11466_00581) - 565178..565504 (+) 327 WP_008461534.1 YggL family protein -
  EL098_RS02880 (NCTC11466_00582) - 565574..566290 (+) 717 WP_126354598.1 DUF2884 domain-containing protein -
  EL098_RS02885 (NCTC11466_00583) hemW 566390..567532 (-) 1143 WP_126354600.1 radical SAM family heme chaperone HemW -
  EL098_RS02890 (NCTC11466_00584) - 567525..568118 (-) 594 WP_126354602.1 XTP/dITP diphosphatase -
  EL098_RS02895 (NCTC11466_00585) yggU 568133..568432 (-) 300 WP_126354604.1 DUF167 family protein YggU -
  EL098_RS02900 (NCTC11466_00586) - 568429..568983 (-) 555 WP_126354606.1 YggT family protein -
  EL098_RS02905 (NCTC11466_00587) - 569008..569712 (-) 705 WP_126354608.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL098_RS02910 (NCTC11466_00588) pilT 569729..570754 (+) 1026 WP_126354610.1 type IV pilus twitching motility protein PilT Machinery gene
  EL098_RS02915 - 570815..570997 (+) 183 WP_126354612.1 type II toxin-antitoxin system HicA family toxin -
  EL098_RS02920 (NCTC11466_00589) - 571021..571476 (+) 456 WP_126354614.1 type II toxin-antitoxin system HicB family antitoxin -
  EL098_RS02925 (NCTC11466_00590) ruvX 571482..571901 (-) 420 WP_126354616.1 Holliday junction resolvase RuvX -
  EL098_RS02930 (NCTC11466_00591) - 571901..572464 (-) 564 WP_197718536.1 YqgE/AlgH family protein -
  EL098_RS02935 (NCTC11466_00592) gshB 572594..573541 (-) 948 WP_039297932.1 glutathione synthase -
  EL098_RS02940 (NCTC11466_00593) rsmE 573554..574285 (-) 732 WP_126354620.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EL098_RS02945 (NCTC11466_00594) endA 574400..575107 (-) 708 WP_126354622.1 deoxyribonuclease I -
  EL098_RS02950 (NCTC11466_00595) - 575200..575715 (-) 516 WP_126354625.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37276.89 Da        Isoelectric Point: 6.9191

>NTDB_id=1118913 EL098_RS02910 WP_126354610.1 569729..570754(+) (pilT) [Cedecea lapagei strain NCTC11466]
MDIEEIVALSVKHNVSDLHLSARQTARWRKCGRLEPCPERVPKVEDVIEAWLNEAQRALLKRAGQLDFSLTLANGVRLRG
NAFLQLEGYSLALRLLASSCPTLEALKTPLHMVELLRQSEGLILVTGATGSGKSTTLAAMIQHLNQHLDGHVLTVEDPIE
FIHPSGRCLIQQREPGRHCESFASALKAALREDPDVILLGELRDRETIALALTAAETGHLVLATLHTRGAAQAVERLVDV
FPAEEKNLIRAQLAGSLKAVLAQKLVSACDGGRVALYELLINTPAVANLIREGKTHQLPGVLQTGQQYGMQSFAHSEAQW
RREGKIAEGEPPGRGPWEAIF

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=1118913 EL098_RS02910 WP_126354610.1 569729..570754(+) (pilT) [Cedecea lapagei strain NCTC11466]
ATGGATATCGAAGAAATCGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTCCGCACGTCAGACGGCACG
TTGGCGAAAGTGCGGCAGGCTGGAGCCATGCCCGGAGAGGGTGCCAAAGGTCGAAGACGTTATTGAGGCATGGCTGAATG
AAGCGCAGAGGGCGCTGCTTAAACGCGCCGGGCAGCTGGATTTTTCCCTTACGCTTGCGAACGGCGTCAGGCTGAGGGGG
AATGCCTTCTTACAGCTGGAGGGGTACTCGTTGGCGCTGAGATTGCTGGCGTCGTCTTGCCCGACGCTGGAAGCCCTGAA
GACCCCCCTCCATATGGTTGAGCTTTTGCGTCAAAGCGAGGGGCTGATACTGGTGACCGGCGCCACCGGCAGCGGAAAAT
CTACAACGCTTGCCGCGATGATCCAGCACCTCAACCAGCATCTTGATGGCCACGTGTTGACGGTGGAAGACCCCATCGAA
TTTATTCATCCCTCTGGCCGCTGCCTGATTCAGCAGCGTGAGCCGGGCCGTCACTGTGAAAGCTTTGCCTCGGCCCTAAA
AGCCGCTTTGAGGGAGGATCCCGATGTCATCCTGCTGGGCGAACTCCGTGACAGAGAGACGATTGCTCTGGCGTTAACCG
CGGCGGAAACCGGGCACTTAGTGCTGGCCACTTTGCACACGCGAGGGGCGGCTCAGGCCGTAGAGCGGCTGGTGGATGTG
TTTCCTGCCGAGGAGAAAAACCTGATTCGAGCTCAGCTTGCCGGCAGCCTTAAGGCCGTGCTGGCGCAGAAGCTGGTATC
GGCATGCGACGGTGGCCGGGTGGCGCTTTACGAGCTGCTGATCAATACCCCGGCCGTGGCAAACCTGATTCGTGAAGGGA
AAACTCATCAACTTCCGGGAGTGCTGCAAACCGGCCAGCAGTACGGTATGCAAAGTTTTGCCCACAGCGAGGCGCAATGG
CGGCGTGAAGGCAAAATCGCGGAAGGTGAGCCGCCAGGCCGCGGGCCATGGGAGGCGATATTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4MBM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.961

98.827

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.961

98.827

0.484
  pilT Neisseria gonorrhoeae MS11

49.547

97.067

0.481
  pilT Neisseria meningitidis 8013

49.547

97.067

0.481
  pilT Acinetobacter baylyi ADP1

47.273

96.774

0.457
  pilT Legionella pneumophila strain ERS1305867

49.206

92.375

0.455
  pilT Legionella pneumophila strain Lp02

49.206

92.375

0.455
  pilT Pseudomonas stutzeri DSM 10701

48.125

93.842

0.452
  pilT Pseudomonas aeruginosa PAK

48.125

93.842

0.452
  pilT Acinetobacter baumannii strain A118

47.812

93.842

0.449
  pilT Acinetobacter nosocomialis M2

47.812

93.842

0.449
  pilT Acinetobacter baumannii D1279779

47.812

93.842

0.449
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.037

95.894

0.422
  pilU Pseudomonas stutzeri DSM 10701

40.663

97.361

0.396
  pilU Vibrio cholerae strain A1552

40.785

97.067

0.396
  pilU Acinetobacter baylyi ADP1

38.994

93.255

0.364

Multiple sequence alignment