Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL064_RS02725 Genome accession   NZ_LR134178
Coordinates   613883..614995 (+) Length   370 a.a.
NCBI ID   WP_101899517.1    Uniprot ID   -
Organism   Legionella sainthelensi strain NCTC12450     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 608883..619995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL064_RS02700 (NCTC12450_00572) bioC 609098..609955 (+) 858 WP_115700488.1 malonyl-ACP O-methyltransferase BioC -
  EL064_RS02705 (NCTC12450_00574) - 610162..610959 (-) 798 WP_128129512.1 LirA/MavJ family T4SS effector -
  EL064_RS02710 (NCTC12450_00575) - 611090..611971 (-) 882 WP_027270758.1 polyprenyl synthetase family protein -
  EL064_RS02715 (NCTC12450_00576) - 611964..612182 (-) 219 WP_101899515.1 exodeoxyribonuclease VII small subunit -
  EL064_RS02720 (NCTC12450_00577) radA 612358..613710 (+) 1353 WP_115700494.1 DNA repair protein RadA -
  EL064_RS02725 (NCTC12450_00578) pilU 613883..614995 (+) 1113 WP_101899517.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL064_RS02730 (NCTC12450_00579) ankK 615055..616971 (+) 1917 WP_128129513.1 Dot/Icm T4SS effector AnkK/LegA5 -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41423.82 Da        Isoelectric Point: 5.2973

>NTDB_id=1118796 EL064_RS02725 WP_101899517.1 613883..614995(+) (pilU) [Legionella sainthelensi strain NCTC12450]
MDITPFFKLMVDKSASDLFFSVGAPPNIKIEGVILPIGHVPLKAQQMAEIALSLMNDDQRKEFEATMELNMGLSIPAVGR
FRINLFRQRGDISMVVRYIKNKIPSIEQLNLPPILKSIVMELRGLVLVVGATGSGKSTTLAAMIDYRNQNQSGHILTIED
PIEFLHQHKKSIVDQREVGIDTMSYDNALVNAMREAPDVILIGEIRERNAMKHAIAYAETGHLCISTLHANNANQAMDRI
INFFPEDARKQVLLDLSLNLRAIISMRLIPGVNQQRMVAVEILLNTPYVSDLIEKGKIDEIKEVMERSTEQGMQTFDQAL
LELYKNGLISEENAIKYADSKNNVGLQIRLNSGKNFDSPEGDLTIESDEH

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1118796 EL064_RS02725 WP_101899517.1 613883..614995(+) (pilU) [Legionella sainthelensi strain NCTC12450]
ATGGACATAACACCTTTTTTTAAATTAATGGTGGATAAAAGTGCTTCAGACCTCTTTTTTAGTGTAGGCGCTCCACCGAA
TATTAAAATTGAGGGGGTTATTTTGCCCATTGGCCATGTTCCGCTTAAAGCCCAACAGATGGCTGAAATTGCTTTGTCTT
TGATGAATGATGATCAGCGTAAAGAATTCGAAGCCACAATGGAGCTGAATATGGGACTTTCGATTCCTGCAGTAGGGCGG
TTTAGGATTAATCTATTTCGTCAACGCGGTGATATCTCTATGGTGGTGCGCTATATTAAAAATAAAATTCCTTCTATAGA
GCAATTAAACTTACCACCTATTTTAAAGTCTATAGTCATGGAATTGCGTGGTTTAGTTTTAGTTGTTGGTGCGACTGGGT
CAGGGAAATCAACAACTCTCGCAGCAATGATTGATTATCGTAATCAGAATCAAAGTGGGCATATTTTAACAATCGAAGAT
CCTATAGAGTTTCTCCATCAGCACAAAAAATCCATAGTAGATCAGCGTGAAGTAGGCATAGATACGATGAGTTACGATAA
TGCATTAGTCAATGCAATGCGAGAGGCGCCGGATGTGATTTTAATCGGTGAAATTCGCGAGCGCAATGCAATGAAACATG
CAATCGCTTACGCAGAAACAGGCCATTTATGCATTTCTACTTTGCATGCGAATAATGCAAATCAAGCGATGGATCGGATC
ATTAATTTTTTTCCTGAGGATGCAAGAAAGCAAGTGCTTTTGGACTTATCACTGAATTTAAGAGCTATTATTTCCATGCG
TCTCATTCCTGGGGTAAACCAGCAACGAATGGTGGCTGTAGAGATCTTATTAAATACGCCTTATGTTTCTGATTTAATTG
AGAAAGGAAAAATTGATGAGATTAAAGAAGTTATGGAGCGGAGTACCGAGCAAGGAATGCAGACCTTTGATCAGGCTTTA
TTAGAGCTTTATAAAAATGGTTTGATTTCAGAGGAAAATGCTATTAAATATGCAGATTCCAAAAATAATGTGGGCTTACA
AATTCGTTTGAATAGTGGTAAAAATTTTGATTCGCCAGAGGGTGACTTAACTATAGAATCGGATGAGCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.514

100

0.535
  pilU Acinetobacter baylyi ADP1

52.661

96.486

0.508
  pilU Vibrio cholerae strain A1552

48.189

97.027

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

91.622

0.405
  pilT Legionella pneumophila strain ERS1305867

42.982

92.432

0.397
  pilT Legionella pneumophila strain Lp02

42.982

92.432

0.397
  pilT Neisseria gonorrhoeae MS11

41.787

93.784

0.392
  pilT Neisseria meningitidis 8013

41.787

93.784

0.392
  pilT Pseudomonas aeruginosa PAK

42.274

92.703

0.392
  pilT Pseudomonas stutzeri DSM 10701

41.399

92.703

0.384
  pilT Vibrio cholerae strain A1552

42.553

88.919

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.553

88.919

0.378
  pilT Acinetobacter baumannii strain A118

41.617

90.27

0.376
  pilT Acinetobacter nosocomialis M2

41.617

90.27

0.376
  pilT Acinetobacter baumannii D1279779

41.617

90.27

0.376
  pilT Acinetobacter baylyi ADP1

40.294

91.892

0.37

Multiple sequence alignment