Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL022_RS08180 Genome accession   NZ_LR134173
Coordinates   1907542..1908576 (+) Length   344 a.a.
NCBI ID   WP_028382153.1    Uniprot ID   A0A0W0S645
Organism   Legionella cherrii strain NCTC11976     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1902542..1913576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL022_RS08150 (NCTC11976_01696) - 1902777..1903010 (-) 234 WP_028382159.1 hypothetical protein -
  EL022_RS08155 (NCTC11976_01697) - 1903143..1904657 (-) 1515 WP_028382158.1 hypothetical protein -
  EL022_RS08160 (NCTC11976_01698) - 1904964..1905245 (-) 282 WP_028382157.1 hypothetical protein -
  EL022_RS08165 (NCTC11976_01699) - 1905381..1905947 (-) 567 WP_028382156.1 DUF2845 domain-containing protein -
  EL022_RS08170 (NCTC11976_01700) - 1905994..1906569 (-) 576 WP_028382155.1 YggT family protein -
  EL022_RS08175 (NCTC11976_01701) - 1906617..1907303 (-) 687 WP_028382154.1 YggS family pyridoxal phosphate-dependent enzyme -
  EL022_RS08180 (NCTC11976_01702) pilT 1907542..1908576 (+) 1035 WP_028382153.1 type IV pilus twitching motility protein PilT Machinery gene
  EL022_RS08185 (NCTC11976_01703) rph 1908732..1909439 (-) 708 WP_028382152.1 ribonuclease PH -
  EL022_RS16605 - 1909706..1909840 (+) 135 WP_272947064.1 hypothetical protein -
  EL022_RS08190 (NCTC11976_01704) - 1909925..1910791 (+) 867 WP_028382151.1 MC/SLC25 family protein -
  EL022_RS08195 (NCTC11976_01705) - 1911206..1912072 (+) 867 WP_028382150.1 YicC/YloC family endoribonuclease -
  EL022_RS08200 (NCTC11976_01706) gmk 1912078..1912707 (+) 630 WP_028382149.1 guanylate kinase -
  EL022_RS08205 (NCTC11976_01707) rpoZ 1912739..1912942 (+) 204 WP_010653170.1 DNA-directed RNA polymerase subunit omega -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38321.17 Da        Isoelectric Point: 6.6655

>NTDB_id=1118770 EL022_RS08180 WP_028382153.1 1907542..1908576(+) (pilT) [Legionella cherrii strain NCTC11976]
MDIAELLAFSVKNKSSDLHISAGLPPLIRVDGDLRKINLPAMEHKEVIKIIYDVMNDRQRKEFEESLETDFSFEITNLAR
FRVNVFHQLRGAAAVFRTIPSEILSMSDLGLPSIFEEMSSYPKGLVLITGPTGSGKSTTLAAMIDYINSSRYDHILTVED
PIEFVHQSKKCLLNQREVHRDTLSFNAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQTLLKKNGGGRVAALEIMICTGAIRNLIREDKVAQMYSSIQTGQGKGMQTLDQHL
TELVKKNIISTHTAREVAIDKNIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1118770 EL022_RS08180 WP_028382153.1 1907542..1908576(+) (pilT) [Legionella cherrii strain NCTC11976]
ATGGATATAGCAGAATTATTAGCGTTTTCAGTAAAAAATAAATCTTCGGACTTACATATCTCTGCGGGATTGCCTCCCTT
AATTCGTGTGGATGGGGATTTACGTAAAATTAATTTGCCGGCCATGGAACACAAGGAAGTAATAAAAATCATCTATGATG
TGATGAATGATAGGCAAAGGAAAGAGTTTGAAGAGAGTTTGGAAACCGATTTTTCCTTTGAAATAACCAATTTGGCTCGC
TTTAGGGTGAACGTATTTCATCAATTACGTGGTGCAGCCGCTGTTTTTCGTACTATTCCTTCTGAAATTTTAAGTATGAG
CGATTTGGGATTACCCTCTATTTTCGAGGAAATGTCCAGTTACCCTAAAGGATTAGTGCTTATTACTGGTCCAACAGGGT
CAGGAAAAAGTACTACTTTGGCCGCCATGATCGATTACATTAATTCCAGTCGCTACGATCATATTTTAACTGTTGAGGAT
CCAATAGAATTTGTACATCAAAGTAAAAAGTGCTTACTCAATCAGCGCGAGGTTCATCGAGATACACTGAGTTTTAATGC
TGCTTTGCGTTCTGCACTGCGTGAAGATCCTGATGTGATTCTGGTGGGGGAATTGCGTGATTTGGAAACGATCCGTTTAG
CCATGACTGCTGCTGAGACAGGACACTTGGTGTTTGGGACCTTGCATACTAATTCGGCAACTAAAACCATTAACCGGATT
ATCGATGTGTTTCCCGCCGAAGAAAAATCCATGGTACGTTCCATGTTGTCTGAGTCATTGCAAGCTGTAGTTGCACAAAC
GCTATTGAAAAAAAATGGAGGTGGGCGAGTTGCTGCTTTGGAAATCATGATTTGCACTGGCGCAATACGAAACTTAATTC
GCGAAGATAAAGTGGCTCAAATGTATTCTTCCATTCAAACAGGCCAGGGTAAAGGAATGCAAACTTTGGATCAGCATTTA
ACAGAGCTGGTGAAAAAAAATATTATTTCCACTCATACGGCACGTGAGGTGGCTATTGATAAAAACATATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0S645

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

88.663

100

0.887
  pilT Legionella pneumophila strain Lp02

88.663

100

0.887
  pilT Acinetobacter baylyi ADP1

76.627

98.256

0.753
  pilT Acinetobacter baumannii strain A118

76.036

98.256

0.747
  pilT Acinetobacter nosocomialis M2

76.036

98.256

0.747
  pilT Acinetobacter baumannii D1279779

76.036

98.256

0.747
  pilT Pseudomonas stutzeri DSM 10701

74.852

98.256

0.735
  pilT Pseudomonas aeruginosa PAK

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.781

95.64

0.648
  pilT Vibrio cholerae strain A1552

67.781

95.64

0.648
  pilT Neisseria meningitidis 8013

64.897

98.547

0.64
  pilT Neisseria gonorrhoeae MS11

64.602

98.547

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.494
  pilU Vibrio cholerae strain A1552

41.691

99.709

0.416
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.157

96.512

0.378

Multiple sequence alignment