Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PATL70BA_RS16075 Genome accession   NZ_LR130778
Coordinates   3504063..3505115 (+) Length   350 a.a.
NCBI ID   WP_125138340.1    Uniprot ID   -
Organism   Petrocella atlantisensis isolate 70B-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3499063..3510115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PATL70BA_RS16060 (PATL70BA_3430) - 3500376..3501038 (+) 663 WP_125138337.1 O-methyltransferase -
  PATL70BA_RS16065 (PATL70BA_3431) - 3501031..3502272 (+) 1242 WP_125138338.1 U32 family peptidase -
  PATL70BA_RS16070 (PATL70BA_3432) - 3502340..3504040 (+) 1701 WP_125138339.1 GspE/PulE family protein -
  PATL70BA_RS16075 (PATL70BA_3433) pilT 3504063..3505115 (+) 1053 WP_125138340.1 type IV pilus twitching motility protein PilT Machinery gene
  PATL70BA_RS16080 (PATL70BA_3434) - 3505359..3506570 (+) 1212 WP_125138341.1 type II secretion system F family protein -
  PATL70BA_RS16085 (PATL70BA_3435) - 3506672..3507106 (+) 435 WP_125138342.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PATL70BA_RS16090 - 3507110..3507850 (+) 741 WP_125138343.1 A24 family peptidase -
  PATL70BA_RS16095 (PATL70BA_3437) pilM 3507884..3509470 (+) 1587 WP_172596302.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38646.69 Da        Isoelectric Point: 7.2372

>NTDB_id=1117569 PATL70BA_RS16075 WP_125138340.1 3504063..3505115(+) (pilT) [Petrocella atlantisensis isolate 70B-A]
MITIEELLGLAKDKKASDVHITVGVPPILRINGDLEYLPYDIMLPDTSKALILPIMIEEQRKVFDKNGEVDFSFAIPSLG
RFRVNAFMQRGSVAAAFRLVGSEIPSPTALGMPSSIQDLYTKKRGLVLVTGPTGSGKSTTLASFINEINQRRSAHVITLE
DPIEYLHKHKKSIVNQREIGLDTFSYANALRAALRQDPDVILVGEMRDLETIAIAITAAETGHLVLSTLHTIGATNTIDR
IIDVFPPHQQQQVRVQLSAVLTAVVSQQLIPTLDGKGRCAAFEVMHANPAVRNLIRENKTHQILSIMQTNKRLGMQTMDD
AILELLRTASISRDDALTFAQDPANLERRM

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=1117569 PATL70BA_RS16075 WP_125138340.1 3504063..3505115(+) (pilT) [Petrocella atlantisensis isolate 70B-A]
ATGATTACCATCGAAGAACTTCTTGGCTTAGCCAAAGATAAAAAAGCTTCCGACGTTCATATAACCGTAGGGGTACCGCC
TATACTTAGAATCAACGGTGATCTTGAATACCTACCCTACGATATCATGCTACCGGATACATCCAAAGCTTTGATTCTTC
CTATCATGATAGAAGAACAAAGAAAAGTTTTTGACAAAAACGGAGAAGTAGACTTCTCTTTTGCCATACCCAGTTTGGGA
CGATTTAGAGTAAATGCTTTTATGCAGAGAGGCTCCGTTGCGGCAGCCTTTCGTCTTGTTGGTTCGGAAATTCCTAGTCC
GACAGCACTGGGCATGCCAAGCTCAATTCAGGATCTCTATACCAAGAAAAGAGGCTTAGTCCTGGTAACAGGACCAACAG
GTAGTGGTAAGTCCACAACCTTAGCCTCTTTTATTAATGAGATTAATCAGCGTAGGAGTGCTCATGTTATAACCCTTGAA
GATCCTATAGAATATCTGCATAAGCACAAAAAAAGTATCGTCAATCAAAGAGAAATCGGTTTGGACACTTTCAGTTATGC
CAATGCACTAAGAGCAGCACTTAGACAAGATCCGGACGTGATTCTTGTAGGTGAGATGCGGGACCTTGAAACCATTGCCA
TTGCCATAACGGCAGCAGAGACAGGCCATCTTGTATTATCAACCTTGCATACCATCGGAGCGACCAACACCATTGACAGG
ATTATAGACGTCTTTCCACCCCATCAACAACAACAGGTGCGTGTTCAGTTGTCTGCAGTATTAACTGCGGTGGTATCTCA
GCAACTTATACCGACCCTCGATGGCAAAGGACGATGTGCGGCTTTTGAAGTTATGCATGCCAATCCGGCGGTGCGTAATC
TAATTCGTGAGAACAAAACCCATCAGATCCTATCCATTATGCAGACCAACAAAAGATTGGGTATGCAAACCATGGATGAT
GCCATCTTAGAGCTGCTTAGAACCGCCAGCATCTCCAGAGATGATGCTCTAACCTTTGCACAGGATCCTGCTAATCTTGA
AAGAAGAATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.738

99.143

0.523
  pilT Neisseria meningitidis 8013

51.594

98.571

0.509
  pilT Neisseria gonorrhoeae MS11

51.304

98.571

0.506
  pilT Legionella pneumophila strain Lp02

51.952

95.143

0.494
  pilT Legionella pneumophila strain ERS1305867

51.952

95.143

0.494
  pilT Pseudomonas stutzeri DSM 10701

50.146

98

0.491
  pilT Pseudomonas aeruginosa PAK

49.271

98

0.483
  pilT Acinetobacter baylyi ADP1

48.105

98

0.471
  pilT Acinetobacter nosocomialis M2

47.522

98

0.466
  pilT Acinetobacter baumannii strain A118

47.522

98

0.466
  pilT Acinetobacter baumannii D1279779

47.522

98

0.466
  pilT Vibrio cholerae strain A1552

50.464

92.286

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.464

92.286

0.466
  pilU Pseudomonas stutzeri DSM 10701

43.478

98.571

0.429
  pilU Acinetobacter baylyi ADP1

43.567

97.714

0.426
  pilU Vibrio cholerae strain A1552

43.807

94.571

0.414

Multiple sequence alignment