Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KR485_RS15650 Genome accession   NZ_LR027557
Coordinates   3526946..3527980 (-) Length   344 a.a.
NCBI ID   WP_032878931.1    Uniprot ID   -
Organism   Pseudomonas synxantha strain 10586     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3521946..3532980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KR485_RS15620 ruvX 3522026..3522463 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  KR485_RS15625 pyrR 3522596..3523102 (+) 507 WP_005792260.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  KR485_RS15630 - 3523127..3524131 (+) 1005 WP_032878936.1 aspartate carbamoyltransferase catalytic subunit -
  KR485_RS15635 - 3524128..3525399 (+) 1272 WP_148854564.1 dihydroorotase -
  KR485_RS15640 - 3525482..3525910 (-) 429 WP_032878933.1 TM2 domain-containing protein -
  KR485_RS15645 - 3526160..3526798 (+) 639 WP_005792265.1 C40 family peptidase -
  KR485_RS15650 pilT 3526946..3527980 (-) 1035 WP_032878931.1 type IV pilus twitching motility protein PilT Machinery gene
  KR485_RS15655 - 3528038..3528733 (+) 696 WP_005792267.1 YggS family pyridoxal phosphate-dependent enzyme -
  KR485_RS15660 proC 3528759..3529577 (+) 819 WP_216090434.1 pyrroline-5-carboxylate reductase -
  KR485_RS15665 - 3529603..3530190 (+) 588 WP_003195087.1 YggT family protein -
  KR485_RS15670 - 3530412..3531551 (+) 1140 WP_005792271.1 homoserine O-acetyltransferase -
  KR485_RS15675 metW 3531559..3532179 (+) 621 WP_005792273.1 methionine biosynthesis protein MetW -
  KR485_RS15680 - 3532201..3532635 (+) 435 WP_078822557.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37443.36 Da        Isoelectric Point: 7.2665

>NTDB_id=1115753 KR485_RS15650 WP_032878931.1 3526946..3527980(-) (pilT) [Pseudomonas synxantha strain 10586]
MDITQLLTASVSRGACDLHLSAGLAPMVRVDGEVWPMDEPVLSAAQLVDLVSPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQNRGIGAVFRAIPTEVQSLEDLGLGEVFQRMAQFPRGLVLVTGPTGSGKSTTLAAMIDYLNRHRRQHILTLED
PIEFIHRPKLALITQRQVHRDTHSFCVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGLVSREEAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1115753 KR485_RS15650 WP_032878931.1 3526946..3527980(-) (pilT) [Pseudomonas synxantha strain 10586]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGTGGCGCCTGTGACCTGCATTTGTCGGCCGGCCTGGCGCCGAT
GGTGCGAGTCGATGGCGAGGTCTGGCCGATGGATGAGCCGGTGCTGAGCGCAGCGCAACTGGTCGATCTGGTGAGCCCTT
TGTTGAATAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAATTGCCGGGAGTCGCACGC
TTTCGGGCGAACGTGTTCCGCCAGAATCGCGGTATAGGCGCGGTGTTTCGTGCCATCCCGACCGAGGTCCAGAGCCTGGA
AGACCTCGGGTTGGGCGAGGTATTCCAGCGTATGGCTCAGTTCCCCCGTGGCCTGGTGCTGGTCACCGGGCCTACGGGTT
CGGGCAAGTCCACGACGCTGGCGGCGATGATCGATTATCTGAACCGGCACCGGCGCCAGCACATCCTCACGCTTGAAGAT
CCCATCGAATTTATCCACAGGCCGAAACTGGCCCTGATCACCCAACGCCAGGTGCACCGCGACACCCACAGCTTCTGCGT
GGCCCTGCGCTCGGCCCTGAGGGAAGACCCGGATGTGATCCTGGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CCTTGACGGCAGCTGAGACCGGGCATCTGGTGTTTGGCACCCTGCACACTTCATCGGCTGCAAAGACTGTAGACAGGCTG
GTGGACGTCTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCGATGTTGTCGGAGTCGTTGCAGGCGGTGGTGTCTCAGGT
ACTGGTGAAGAAGGTCGGCGGCGGCCGTGTGGCCGCCCATGAAATCATGCTGGGCACGCCGGCGATTCGCAATCTGATCC
GTGAGGACAAGGTGGCGCAGATGGTTTCGGCAATTCAGACCGGTGGCGCGTTGGGGATGAAGACCCTGGATATGAGTTTG
AAGGCGTTGGTCGGCGCGGGGCTGGTCAGCCGGGAAGAGGCGCGGGAGAAGGCGAGGGTGCCTGCAGATATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.093

100

0.721
  pilT Pseudomonas stutzeri DSM 10701

70.349

100

0.703
  pilT Acinetobacter baumannii D1279779

68.915

99.128

0.683
  pilT Acinetobacter baumannii strain A118

68.915

99.128

0.683
  pilT Acinetobacter nosocomialis M2

68.915

99.128

0.683
  pilT Acinetobacter baylyi ADP1

67.742

99.128

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.095

97.965

0.628
  pilT Vibrio cholerae strain A1552

64.095

97.965

0.628
  pilT Legionella pneumophila strain Lp02

63.609

98.256

0.625
  pilT Legionella pneumophila strain ERS1305867

63.609

98.256

0.625
  pilT Neisseria meningitidis 8013

61.471

98.837

0.608
  pilT Neisseria gonorrhoeae MS11

61.176

98.837

0.605
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.491
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

37.572

100

0.378

Multiple sequence alignment