Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CCOS191_RS01865 Genome accession   NZ_LN847264
Coordinates   380473..381465 (+) Length   330 a.a.
NCBI ID   WP_046853836.1    Uniprot ID   -
Organism   Pseudomonas sp. CCOS 191     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375473..386465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCOS191_RS01835 (CCOS191_0367) - 375949..376383 (-) 435 WP_046853831.1 DUF4426 domain-containing protein -
  CCOS191_RS01840 (CCOS191_0368) metW 376406..377026 (-) 621 WP_046853832.1 methionine biosynthesis protein MetW -
  CCOS191_RS01845 (CCOS191_0369) - 377034..378173 (-) 1140 WP_046853833.1 homoserine O-acetyltransferase -
  CCOS191_RS01850 (CCOS191_0370) - 378262..378852 (-) 591 WP_023630880.1 YggT family protein -
  CCOS191_RS01855 (CCOS191_0371) proC 378863..379681 (-) 819 WP_046853834.1 pyrroline-5-carboxylate reductase -
  CCOS191_RS01860 (CCOS191_0372) - 379721..380407 (-) 687 WP_046853835.1 YggS family pyridoxal phosphate-dependent enzyme -
  CCOS191_RS01865 (CCOS191_0373) pilT 380473..381465 (+) 993 WP_046853836.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CCOS191_RS01870 (CCOS191_0374) - 381514..382161 (-) 648 WP_046853837.1 NlpC/P60 family protein -
  CCOS191_RS01875 (CCOS191_0375) - 382319..382744 (+) 426 WP_023630210.1 TM2 domain-containing protein -
  CCOS191_RS01880 (CCOS191_0376) - 382784..383488 (-) 705 WP_023630209.1 SPOR domain-containing protein -
  CCOS191_RS01885 (CCOS191_0377) argS 383490..385226 (-) 1737 WP_046853838.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35504.12 Da        Isoelectric Point: 6.5017

>NTDB_id=1114522 CCOS191_RS01865 WP_046853836.1 380473..381465(+) (pilT) [Pseudomonas sp. CCOS 191]
MDVTDLLGRAVAAGASDLHLAVGEPPLLRLDGELQRMAVPALTCAALEQGMAGLLDDGQRRQWLSGDELDLALSLPGVGR
LRLNLFRQHNGLGASARLIPARVPSLDELELNDVYQAVAQCRDGLVLVGGPTGSGKSSTLAALLDQINRERALQVITLED
PIEFIHSGQGCLVNQREIGMHSRDFAQGLRSALRQDPDVIMVGELRDLESIRLALRAAETGHLVLATVHTRSAVNSIDRL
VEVFAAEEKPLVRMMLAESLRLVVAQVLIRRVGGGRVAAREVLIATPAVRNLIREGRMAQVQSVMQAGAALGMQTMEGAM
RALRQKGLVD

Nucleotide


Download         Length: 993 bp        

>NTDB_id=1114522 CCOS191_RS01865 WP_046853836.1 380473..381465(+) (pilT) [Pseudomonas sp. CCOS 191]
ATGGATGTGACCGACCTGCTGGGCCGGGCCGTGGCCGCAGGCGCCAGCGACCTGCACCTGGCCGTGGGCGAGCCGCCGTT
GCTGCGCCTGGATGGCGAGTTGCAGCGCATGGCGGTGCCGGCGCTGACCTGCGCAGCTCTGGAACAGGGAATGGCCGGCC
TGCTCGATGACGGGCAGCGGCGGCAATGGCTGAGCGGTGATGAACTGGACCTGGCGCTGAGCTTGCCCGGCGTGGGGCGC
CTGCGCTTGAACCTGTTCCGCCAGCACAATGGATTGGGCGCAAGCGCGCGCCTGATCCCGGCGCGGGTCCCGAGCCTCGA
TGAACTTGAACTGAACGATGTGTATCAAGCCGTTGCGCAATGTCGGGATGGCTTGGTGCTGGTGGGCGGGCCGACGGGTA
GCGGCAAGTCCAGCACCCTGGCGGCGCTGCTCGATCAGATCAATCGCGAACGCGCGCTGCAGGTCATCACCCTCGAAGAC
CCTATCGAATTTATCCACAGCGGCCAGGGTTGCCTGGTCAATCAGCGGGAGATCGGCATGCACAGCCGTGACTTCGCCCA
AGGGTTGCGCAGTGCCCTGCGCCAGGATCCGGATGTGATCATGGTGGGTGAGTTGCGTGATCTGGAGAGTATCCGCCTTG
CATTGCGCGCGGCAGAAACCGGGCATCTGGTGCTGGCAACGGTGCACACCCGCTCGGCGGTGAACAGCATCGACCGGCTG
GTAGAGGTGTTCGCGGCCGAGGAGAAGCCGCTGGTGCGCATGATGCTGGCTGAATCACTGCGCCTGGTGGTGGCTCAGGT
GCTGATCAGGCGGGTGGGCGGCGGCAGGGTCGCCGCAAGGGAGGTGCTGATAGCGACGCCCGCGGTGCGCAATTTGATTC
GCGAGGGGCGCATGGCACAGGTGCAGTCGGTGATGCAGGCGGGGGCGGCATTGGGGATGCAGACGATGGAGGGGGCGATG
CGGGCGCTCAGGCAGAAAGGCCTGGTCGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.368

99.697

0.512
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.909

100

0.509
  pilT Vibrio cholerae strain A1552

50.909

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

50.76

99.697

0.506
  pilT Acinetobacter baylyi ADP1

50.303

100

0.503
  pilT Acinetobacter nosocomialis M2

49.848

99.697

0.497
  pilT Acinetobacter baumannii D1279779

49.848

99.697

0.497
  pilT Acinetobacter baumannii strain A118

49.848

99.697

0.497
  pilT Neisseria meningitidis 8013

47.879

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.576

100

0.476
  pilT Legionella pneumophila strain Lp02

47.416

99.697

0.473
  pilT Legionella pneumophila strain ERS1305867

47.416

99.697

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.152

100

0.452
  pilU Pseudomonas stutzeri DSM 10701

38.11

99.394

0.379
  pilU Vibrio cholerae strain A1552

37.037

98.182

0.364
  pilB/pilB1 Synechocystis sp. PCC 6803

34.294

100

0.361

Multiple sequence alignment