Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CS367_RS12005 Genome accession   NZ_CM008894
Coordinates   2537381..2538403 (-) Length   340 a.a.
NCBI ID   WP_101427354.1    Uniprot ID   -
Organism   Serratia marcescens strain 12TM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2532381..2543403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS367_RS11975 (CS367_11945) - 2533186..2533698 (+) 513 WP_101427353.1 SprT family zinc-dependent metalloprotease -
  CS367_RS11980 (CS367_11950) endA 2533800..2534495 (+) 696 WP_033649591.1 deoxyribonuclease I -
  CS367_RS11985 (CS367_11955) rsmE 2534565..2535296 (+) 732 WP_033649590.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CS367_RS11990 (CS367_11960) gshB 2535307..2536257 (+) 951 WP_033649589.1 glutathione synthase -
  CS367_RS11995 (CS367_11965) - 2536399..2536962 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  CS367_RS12000 (CS367_11970) ruvX 2536962..2537384 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  CS367_RS12005 (CS367_11975) pilT 2537381..2538403 (-) 1023 WP_101427354.1 type IV pilus twitching motility protein PilT Machinery gene
  CS367_RS12010 (CS367_11980) - 2538424..2539131 (+) 708 WP_101427355.1 YggS family pyridoxal phosphate-dependent enzyme -
  CS367_RS12015 (CS367_11985) proC 2539151..2539972 (+) 822 WP_101427356.1 pyrroline-5-carboxylate reductase -
  CS367_RS12020 (CS367_11990) - 2540004..2540558 (+) 555 WP_033635944.1 YggT family protein -
  CS367_RS12025 (CS367_11995) yggU 2540555..2540845 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  CS367_RS12030 (CS367_12000) - 2540863..2541456 (+) 594 WP_033635946.1 XTP/dITP diphosphatase -
  CS367_RS12035 (CS367_12005) hemW 2541449..2542591 (+) 1143 WP_101427357.1 radical SAM family heme chaperone HemW -
  CS367_RS26685 - 2542628..2542714 (-) 87 Protein_2374 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36447.72 Da        Isoelectric Point: 7.3039

>NTDB_id=111384 CS367_RS12005 WP_101427354.1 2537381..2538403(-) (pilT) [Serratia marcescens strain 12TM]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLASSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRTDAGVLGESAGKGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=111384 CS367_RS12005 WP_101427354.1 2537381..2538403(-) (pilT) [Serratia marcescens strain 12TM]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAACTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCAGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTAGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGACCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGAAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Neisseria gonorrhoeae MS11

49.555

99.118

0.491
  pilT Neisseria meningitidis 8013

50.453

97.353

0.491
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Pseudomonas aeruginosa PAK

49.697

97.059

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.512

98.824

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.894

94.706

0.444
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371

Multiple sequence alignment