Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LFA_RS09980 Genome accession   NZ_LN614827
Coordinates   2469338..2470372 (-) Length   344 a.a.
NCBI ID   WP_045096062.1    Uniprot ID   A0A098G7U2
Organism   Legionella fallonii LLAP-10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2464338..2475372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFA_RS09960 (LFA_2207) - 2464637..2465503 (-) 867 WP_045096058.1 YicC/YloC family endoribonuclease -
  LFA_RS09965 (LFA_2208) rph 2465817..2466524 (+) 708 WP_045096059.1 ribonuclease PH -
  LFA_RS09970 (LFA_2210) - 2466738..2467226 (+) 489 WP_045096060.1 hypothetical protein -
  LFA_RS09975 (LFA_2211) - 2467565..2468830 (+) 1266 WP_045096061.1 MFS transporter -
  LFA_RS09980 (LFA_2212) pilT 2469338..2470372 (-) 1035 WP_045096062.1 type IV pilus twitching motility protein PilT Machinery gene
  LFA_RS09985 (LFA_2213) - 2470737..2471438 (+) 702 WP_045096063.1 YggS family pyridoxal phosphate-dependent enzyme -
  LFA_RS09990 (LFA_2214) proC 2471428..2472216 (+) 789 WP_045096064.1 pyrroline-5-carboxylate reductase -
  LFA_RS09995 (LFA_2215) - 2472267..2472842 (+) 576 WP_045096065.1 YggT family protein -
  LFA_RS10000 (LFA_2216) - 2472981..2473541 (+) 561 WP_045096066.1 DUF2845 domain-containing protein -
  LFA_RS10005 (LFA_2217) - 2473703..2473984 (+) 282 WP_045096067.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38235.13 Da        Isoelectric Point: 6.8357

>NTDB_id=1113625 LFA_RS09980 WP_045096062.1 2469338..2470372(-) (pilT) [Legionella fallonii LLAP-10]
MDIAELLAFSVKNKSSDLHISAGLPPMIRVDGDLRKINLPDLDHKTVIKIIYDIMNDRQRKEFEEFLETDFSFEITNLAR
FRVNAFNQSRGAAAVFRTIPSAILSMDDLHLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAMIDFINSSRYEHILTVED
PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKAMVRSMLSESLQAVIAQSLLKKNGGGRVAALEIMICTGAIRNLIREDKVAQMYSSIQTGQSKGMQTLDQHL
TELVGKEIISTHVAREAAINKTLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1113625 LFA_RS09980 WP_045096062.1 2469338..2470372(-) (pilT) [Legionella fallonii LLAP-10]
ATGGACATAGCCGAGTTATTAGCATTTTCCGTCAAGAACAAATCCTCAGATTTACATATATCAGCTGGTTTACCTCCCAT
GATCCGTGTGGACGGGGATTTGCGAAAAATCAATTTGCCTGATTTAGATCATAAAACCGTGATAAAAATCATTTATGACA
TTATGAATGATAGACAAAGAAAGGAGTTCGAAGAGTTTTTGGAAACGGATTTTTCCTTTGAAATTACTAACTTAGCTCGT
TTTAGGGTGAATGCCTTTAATCAATCTAGGGGGGCGGCTGCTGTCTTTCGAACCATTCCTTCTGCTATTTTAAGTATGGA
TGATTTACATTTACCGCCTATCTTCAAGGAAATGGCCAGTTTTTCTAGAGGCTTGGTATTAGTGACAGGGCCGACTGGTT
CGGGGAAAAGTACCACTCTTGCCGCAATGATTGATTTTATTAACTCTTCTCGTTATGAGCACATTTTGACTGTTGAGGAT
CCGATAGAGTTTGTTCATCAGAGCAAAAAATGCCTGGTTAACCAACGTGAAGTCCATAAAGATACTCTTAGTTTCAATGC
CGCGCTACGTTCGGCTTTACGTGAAGATCCGGATATCATTTTAGTGGGGGAATTACGCGATCTGGAAACAATTCGTCTAG
CTATGACTGCTGCAGAAACTGGACACTTGGTTTTTGGCACCTTACATACTAACTCCGCGACCAAAACCATAAACAGAATT
ATTGATGTTTTTCCTGCAGAAGAAAAAGCAATGGTTCGCTCTATGTTATCTGAGTCATTACAAGCAGTCATTGCTCAAAG
CTTATTAAAGAAAAATGGTGGGGGTAGGGTTGCTGCTTTGGAAATTATGATTTGTACAGGGGCTATTCGTAATTTGATAA
GAGAAGACAAGGTAGCGCAAATGTATTCCTCTATTCAAACGGGGCAATCTAAGGGAATGCAGACCTTAGATCAACACCTA
ACGGAATTAGTCGGCAAAGAAATTATCTCCACTCATGTTGCCCGTGAGGCCGCAATTAATAAGACCTTATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098G7U2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

92.733

100

0.927
  pilT Legionella pneumophila strain Lp02

92.733

100

0.927
  pilT Pseudomonas aeruginosa PAK

76.331

98.256

0.75
  pilT Acinetobacter baumannii strain A118

76.331

98.256

0.75
  pilT Acinetobacter nosocomialis M2

76.331

98.256

0.75
  pilT Acinetobacter baumannii D1279779

76.331

98.256

0.75
  pilT Pseudomonas stutzeri DSM 10701

76.036

98.256

0.747
  pilT Acinetobacter baylyi ADP1

75.148

98.256

0.738
  pilT Neisseria meningitidis 8013

65.103

99.128

0.645
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.477

95.64

0.645
  pilT Vibrio cholerae strain A1552

67.477

95.64

0.645
  pilT Neisseria gonorrhoeae MS11

64.516

99.128

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Vibrio cholerae strain A1552

43.068

98.547

0.424
  pilU Acinetobacter baylyi ADP1

42.169

96.512

0.407
  pilU Pseudomonas stutzeri DSM 10701

42.593

94.186

0.401

Multiple sequence alignment