Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   LFA_RS09655 Genome accession   NZ_LN614827
Coordinates   2398422..2398835 (-) Length   137 a.a.
NCBI ID   WP_045096003.1    Uniprot ID   A0A098G7M8
Organism   Legionella fallonii LLAP-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2393422..2403835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFA_RS09635 (LFA_2133) - 2394540..2395724 (+) 1185 WP_052673930.1 LegC2/C7 family Dot/Icm T4SS effector -
  LFA_RS09640 (LFA_2134) - 2396020..2396205 (-) 186 WP_045096000.1 hypothetical protein -
  LFA_RS09645 (LFA_2135) - 2396276..2397742 (-) 1467 WP_045096001.1 hypothetical protein -
  LFA_RS09650 (LFA_2136) pilA2 2397893..2398297 (-) 405 WP_045096002.1 pilin Machinery gene
  LFA_RS09655 (LFA_2137) pilA2 2398422..2398835 (-) 414 WP_045096003.1 pilin Machinery gene
  LFA_RS09660 (LFA_2138) - 2399158..2399481 (+) 324 WP_045096004.1 BolA family transcriptional regulator -
  LFA_RS09665 (LFA_2139) - 2399677..2401077 (+) 1401 WP_045096005.1 APC family permease -
  LFA_RS09670 (LFA_2140) pilA2 2401145..2401558 (-) 414 WP_045096006.1 pilin Machinery gene
  LFA_RS09675 (LFA_2141) kdsB 2401790..2402533 (-) 744 WP_045096007.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  LFA_RS09680 (LFA_2142) - 2402530..2402709 (-) 180 WP_045096008.1 Trm112 family protein -
  LFA_RS09685 (LFA_2143) tsaB 2402702..2403376 (-) 675 WP_231865931.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14123.29 Da        Isoelectric Point: 6.1608

>NTDB_id=1113623 LFA_RS09655 WP_045096003.1 2398422..2398835(-) (pilA2) [Legionella fallonii LLAP-10]
MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLQMASSAQLAVSETTMTNNALPATQTATGYTTPAATPNV
ASIAISNDGLAVITITYSAAAGGGTITLTPTLTTNGDVTWVCKVGTELPKYAPASCR

Nucleotide


Download         Length: 414 bp        

>NTDB_id=1113623 LFA_RS09655 WP_045096003.1 2398422..2398835(-) (pilA2) [Legionella fallonii LLAP-10]
ATGAGACAAAAGGGTTTTACGTTAATTGAATTAATGATAGTTGTGGCAATTATTGGTATTTTAGCCGCTATTGCTATTCC
AGCTTACCAAGACTACACGATTAGAGCGCGTGTGACAGAAGGGTTGCAAATGGCCTCGTCAGCCCAATTAGCCGTTTCAG
AAACGACTATGACGAATAATGCTTTACCTGCTACGCAAACAGCAACAGGCTATACTACTCCCGCAGCGACTCCCAACGTT
GCCTCAATTGCAATTTCTAACGATGGTCTTGCTGTAATCACCATCACTTATAGTGCAGCCGCTGGTGGTGGAACAATTAC
TTTAACACCGACCCTTACCACTAACGGAGATGTGACTTGGGTCTGTAAAGTTGGAACGGAGTTACCTAAATATGCTCCGG
CTAGCTGTAGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098G7M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

81.752

100

0.818
  pilA2 Legionella pneumophila strain ERS1305867

81.022

100

0.81
  pilA Ralstonia pseudosolanacearum GMI1000

46.584

100

0.547
  comP Acinetobacter baylyi ADP1

50

100

0.533
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.413

100

0.489
  pilA/pilA1 Eikenella corrodens VA1

38.217

100

0.438
  pilA Acinetobacter baumannii strain A118

44.776

97.81

0.438
  pilA Pseudomonas aeruginosa PAK

40.268

100

0.438
  pilE Neisseria gonorrhoeae MS11

36.943

100

0.423
  pilA Vibrio cholerae C6706

36.301

100

0.387
  pilA Vibrio cholerae strain A1552

36.301

100

0.387
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.301

100

0.387
  pilA Vibrio parahaemolyticus RIMD 2210633

40

91.241

0.365
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.313

97.81

0.365

Multiple sequence alignment