Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KHO07_RS01705 Genome accession   NZ_HG995459
Coordinates   373151..374185 (+) Length   344 a.a.
NCBI ID   WP_150734159.1    Uniprot ID   A0A5E7KWU2
Organism   Pseudomonas sp. PP3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 368151..379185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHO07_RS01675 - 368530..368964 (-) 435 WP_212626322.1 DUF4426 domain-containing protein -
  KHO07_RS01680 metW 368991..369611 (-) 621 WP_150734155.1 methionine biosynthesis protein MetW -
  KHO07_RS01685 - 369619..370758 (-) 1140 WP_212626323.1 homoserine O-acetyltransferase -
  KHO07_RS01690 - 370942..371529 (-) 588 WP_212626324.1 YggT family protein -
  KHO07_RS01695 proC 371554..372372 (-) 819 WP_150734157.1 pyrroline-5-carboxylate reductase -
  KHO07_RS01700 - 372408..373094 (-) 687 WP_212626325.1 YggS family pyridoxal phosphate-dependent enzyme -
  KHO07_RS01705 pilT 373151..374185 (+) 1035 WP_150734159.1 type IV pilus twitching motility protein PilT Machinery gene
  KHO07_RS01710 - 374327..374944 (-) 618 WP_212626326.1 C40 family peptidase -
  KHO07_RS01715 - 375195..375626 (+) 432 WP_150734161.1 TM2 domain-containing protein -
  KHO07_RS01720 - 375725..376996 (-) 1272 WP_150734162.1 dihydroorotase -
  KHO07_RS01725 - 376993..377997 (-) 1005 WP_020797380.1 aspartate carbamoyltransferase catalytic subunit -
  KHO07_RS01730 pyrR 378025..378528 (-) 504 WP_150734163.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  KHO07_RS01735 ruvX 378623..379060 (-) 438 WP_150734164.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37921.51 Da        Isoelectric Point: 7.1599

>NTDB_id=1112600 KHO07_RS01705 WP_150734159.1 373151..374185(+) (pilT) [Pseudomonas sp. PP3]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVQALIYDIMNDTQRVDFEKHLETDFSFDVPGVAR
FRVNAFNQSRGAGAVFRTIPSRVLTMDELGMGEVFRKMTDAPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQSGGSLGMQTLDMGL
KDLVTKGLVSREHAREKARSPDGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1112600 KHO07_RS01705 WP_150734159.1 373151..374185(+) (pilT) [Pseudomonas sp. PP3]
ATGGATATCACTGAACTGCTCGCGTTCAGCGCCAAACAGGGCGCATCCGACCTGCACCTGTCAGCCGGCCTGCCGCCGAT
GATCAGGGTTGATGGCGATGTACGGCGGATCAATCTGCCGGCACTGGACCACAAGCAAGTGCAGGCGTTGATCTACGACA
TCATGAACGACACCCAGCGCGTCGACTTCGAAAAACACCTGGAAACCGACTTTTCCTTTGATGTGCCCGGCGTGGCGCGT
TTTCGGGTCAACGCGTTCAACCAGAGTCGCGGCGCGGGCGCGGTGTTCCGCACGATCCCGTCCAGAGTCCTGACCATGGA
TGAACTGGGCATGGGCGAGGTGTTTCGCAAGATGACCGACGCGCCTCGTGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CTGGCAAGTCCACCACGCTGGCGGCGATGATCGATTACCTGAACAACCATCGCCATCACCACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGGGAAGTGCACCGCGACACCCGCAGTTTCGCCAC
GGCCCTGCGCTCGGCGCTGCGAGAAGACCCGGACGTGATTCTGGTGGGAGAGATGCGAGATCTGGAAACCATTCGCCTTG
CACTGACCGCAGCCGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCACCTCGGCGGCGAAAACCATCGACCGGATC
GTCGACGTATTTCCCGGGGACGAGAAGTCCATGGTGCGCTCGATGTTGTCCGAGTCGTTGCTGGCGGTGGTGTCGCAGAC
ACTGGTGAAAAAGATCGGCGGCGGGCGCATTGCCGCTCACGAGATCATGCTGGGAACATCGGCGATTCGTAATCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGTCGATCCAGTCGGGTGGGTCGTTGGGGATGCAGACGCTGGATATGGGCTTG
AAGGATTTGGTGACCAAGGGCTTGGTCAGTCGGGAGCATGCGCGGGAGAAGGCGCGGTCACCGGATGGGTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5E7KWU2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

85.756

100

0.858
  pilT Pseudomonas aeruginosa PAK

85.756

100

0.858
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Acinetobacter nosocomialis M2

76.744

100

0.767
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

70.93

100

0.709
  pilT Legionella pneumophila strain ERS1305867

70.93

100

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Neisseria meningitidis 8013

64.928

100

0.651
  pilT Neisseria gonorrhoeae MS11

64.638

100

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

41.399

99.709

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment