Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SMDB11_RS17055 Genome accession   NZ_HG326223
Coordinates   3652775..3653797 (-) Length   340 a.a.
NCBI ID   WP_025304247.1    Uniprot ID   -
Organism   Serratia marcescens subsp. marcescens Db11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3647775..3658797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMDB11_RS17025 (SMDB11_3429) - 3648574..3649086 (+) 513 WP_025304242.1 SprT family zinc-dependent metalloprotease -
  SMDB11_RS17030 (SMDB11_3430) endA 3649188..3649883 (+) 696 WP_025304243.1 deoxyribonuclease I -
  SMDB11_RS17035 (SMDB11_3431) rsmE 3649953..3650684 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SMDB11_RS17040 (SMDB11_3432) gshB 3650695..3651645 (+) 951 WP_025304245.1 glutathione synthase -
  SMDB11_RS17045 (SMDB11_3434) - 3651793..3652356 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  SMDB11_RS17050 (SMDB11_3435) ruvX 3652356..3652778 (+) 423 WP_025304246.1 Holliday junction resolvase RuvX -
  SMDB11_RS17055 (SMDB11_3436) pilT 3652775..3653797 (-) 1023 WP_025304247.1 type IV pilus twitching motility protein PilT Machinery gene
  SMDB11_RS17060 (SMDB11_3437) - 3653818..3654525 (+) 708 WP_025304248.1 YggS family pyridoxal phosphate-dependent enzyme -
  SMDB11_RS17065 (SMDB11_3438) proC 3654545..3655366 (+) 822 WP_025304249.1 pyrroline-5-carboxylate reductase -
  SMDB11_RS17070 (SMDB11_3439) - 3655398..3655952 (+) 555 WP_025304250.1 YggT family protein -
  SMDB11_RS17075 (SMDB11_3440) yggU 3655949..3656239 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  SMDB11_RS17080 (SMDB11_3441) - 3656257..3656850 (+) 594 WP_025304251.1 XTP/dITP diphosphatase -
  SMDB11_RS17085 (SMDB11_3442) hemW 3656843..3657985 (+) 1143 WP_025304252.1 radical SAM family heme chaperone HemW -
  SMDB11_RS17090 (SMDB11_3443) - 3658022..3658270 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -
  SMDB11_RS17095 (SMDB11_3443A) - 3658254..3658496 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36460.65 Da        Isoelectric Point: 7.0670

>NTDB_id=1111957 SMDB11_RS17055 WP_025304247.1 3652775..3653797(-) (pilT) [Serratia marcescens subsp. marcescens Db11]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAQTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANIFQQSAGMSLALRRIAGQSPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSASQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1111957 SMDB11_RS17055 WP_025304247.1 3652775..3653797(-) (pilT) [Serratia marcescens subsp. marcescens Db11]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCAAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACATTTTTCAACAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGTATTGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCAGCGCCGGCCATCGTGCCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCAGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAG
GATCCGATTGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTTGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCACCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGTCGCAGGCAGTGGAACGG
TTAGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATTGACGCCGGTGTGTTGGGGGACTGTGCGGGGGAGGGAACGGGTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Neisseria gonorrhoeae MS11

49.254

98.529

0.485
  pilT Neisseria meningitidis 8013

49.699

97.647

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.353

0.476
  pilT Legionella pneumophila strain Lp02

48.943

97.353

0.476
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment