Detailed information    

insolico Bioinformatically predicted

Overview

Name   comP   Type   Machinery gene
Locus tag   PKB_RS23335 Genome accession   NZ_HG322950
Coordinates   4992790..4993194 (-) Length   134 a.a.
NCBI ID   WP_043254945.1    Uniprot ID   A0A024HN65
Organism   Pseudomonas knackmussii B13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4987790..4998194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PKB_RS23315 (PKB_4737) - 4987934..4989004 (-) 1071 WP_242411187.1 diguanylate cyclase -
  PKB_RS23325 (PKB_4739) - 4989263..4990831 (-) 1569 WP_043254942.1 O-antigen ligase family protein -
  PKB_RS23330 (PKB_4740) - 4990844..4992592 (-) 1749 WP_167333376.1 sulfatase-like hydrolase/transferase -
  PKB_RS23335 (PKB_4741) comP 4992790..4993194 (-) 405 WP_043254945.1 pilin Machinery gene
  PKB_RS23340 (PKB_4742) pilB 4993422..4995125 (+) 1704 WP_043254947.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PKB_RS23345 (PKB_4743) pilC 4995129..4996346 (+) 1218 WP_043254948.1 type II secretion system F family protein Machinery gene
  PKB_RS23350 (PKB_4744) pilD 4996350..4997222 (+) 873 WP_043254951.1 A24 family peptidase Machinery gene
  PKB_RS23355 (PKB_4745) coaE 4997308..4997916 (+) 609 WP_043254953.1 dephospho-CoA kinase -
  PKB_RS23360 (PKB_4746) yacG 4997913..4998122 (+) 210 WP_043254955.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13812.86 Da        Isoelectric Point: 6.2112

>NTDB_id=1111930 PKB_RS23335 WP_043254945.1 4992790..4993194(-) (comP) [Pseudomonas knackmussii B13]
MNAQKGFTLIELMIVVAIIGILAAIALPAYQDYTIRARVSEALVLASGVKATVGENIASNNKIDANSCKGFTDITTATDN
VSSVKCDTGEITVVTTAKAGGVTLTLTPSQASGGPVTWKCATTSDAKYVPAECR

Nucleotide


Download         Length: 405 bp        

>NTDB_id=1111930 PKB_RS23335 WP_043254945.1 4992790..4993194(-) (comP) [Pseudomonas knackmussii B13]
ATGAACGCTCAGAAAGGCTTTACCCTGATCGAACTCATGATCGTGGTTGCGATCATCGGTATCCTGGCTGCCATCGCTCT
GCCGGCCTATCAGGACTACACCATCCGTGCCCGTGTATCGGAAGCTCTGGTTCTGGCATCTGGCGTCAAGGCCACAGTTG
GTGAGAATATCGCGAGCAATAACAAGATTGATGCAAACTCTTGTAAGGGCTTCACTGATATCACTACTGCTACTGATAAC
GTTTCGTCGGTGAAGTGTGATACTGGCGAGATTACTGTAGTGACCACTGCTAAGGCGGGCGGCGTCACTCTGACGCTGAC
TCCTAGCCAAGCCTCTGGCGGCCCTGTCACCTGGAAGTGTGCTACTACTTCTGATGCTAAGTACGTTCCGGCTGAGTGCC
GTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024HN65

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Acinetobacter baylyi ADP1

58.503

100

0.642
  pilA Ralstonia pseudosolanacearum GMI1000

46.584

100

0.56
  pilA2 Legionella pneumophila strain ERS1305867

55.97

100

0.56
  pilA2 Legionella pneumophila str. Paris

55.97

100

0.56
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.957

100

0.507
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.377

100

0.478
  pilE Neisseria gonorrhoeae MS11

39.873

100

0.47
  pilA/pilA1 Eikenella corrodens VA1

40.94

100

0.455
  pilA Pseudomonas aeruginosa PAK

38.411

100

0.433
  pilA Haemophilus influenzae Rd KW20

42.963

100

0.433
  pilA Haemophilus influenzae 86-028NP

42.963

100

0.433
  pilA Acinetobacter baumannii strain A118

38.255

100

0.425
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.706

100

0.403
  pilA Vibrio parahaemolyticus RIMD 2210633

37.405

97.761

0.366

Multiple sequence alignment