Detailed information NTG1111933

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PKB_RS23350
• Genome accession: NZ_HG322950
• Coordinates: 4996350..4997222 (+)
• Length: 290 a.a.
• NCBI ID: WP_043254951.1
• Uniprot ID: -
• Organism: Pseudomonas knackmussii B13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4991350..5002222

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PKB_RS23335 (PKB_4741)	comP	4992790..4993194 (-)	405	WP_043254945.1	pilin	Machinery gene
PKB_RS23340 (PKB_4742)	pilB	4993422..4995125 (+)	1704	WP_043254947.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PKB_RS23345 (PKB_4743)	pilC	4995129..4996346 (+)	1218	WP_043254948.1	type II secretion system F family protein	Machinery gene
PKB_RS23350 (PKB_4744)	pilD	4996350..4997222 (+)	873	WP_043254951.1	A24 family peptidase	Machinery gene
PKB_RS23355 (PKB_4745)	coaE	4997308..4997916 (+)	609	WP_043254953.1	dephospho-CoA kinase	-
PKB_RS23360 (PKB_4746)	yacG	4997913..4998122 (+)	210	WP_043254955.1	DNA gyrase inhibitor YacG	-
PKB_RS23365 (PKB_4747)	-	4998188..4998403 (-)	216	WP_043254956.1	hypothetical protein	-
PKB_RS23370 (PKB_4748)	-	4998510..4999217 (-)	708	WP_043254958.1	energy-coupling factor ABC transporter permease	-
PKB_RS23375 (PKB_4749)	-	4999214..4999681 (-)	468	WP_242411188.1	FAD/FMN-containing dehydrogenase	-
PKB_RS23380 (PKB_4750)	-	4999685..5000101 (-)	417	WP_043254962.1	GNAT family N-acetyltransferase	-
PKB_RS23385 (PKB_4751)	-	5000199..5000828 (+)	630	WP_043254964.1	DUF1780 domain-containing protein	-
PKB_RS23390 (PKB_4752)	-	5000898..5001071 (+)	174	WP_043254973.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32000.92 Da
• Isoelectric Point: 6.8939

>NTDB_id=1111933 PKB_RS23350 WP_043254951.1 4996350..4997222(+) (pilD) [Pseudomonas knackmussii B13]
MPALEFLASHALAFVLCAALLGLLVGSFLNVVIHRLPKMLQLDWEQQARDTLALPHPEKRETYNLILPNSHCPHCDHEIR
PWENIPVVSYLALRGKCSSCKAPISARYPLIEVATALLSAFVAWHFGFTWQAGAMLLLSWGLLAMSMIDVDHQLLPDALV
LPLLWLGLIVNAFGLFTSLSDALWGAVFGYLSLWTVFWVFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AVLGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITTSYLQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=1111933 PKB_RS23350 WP_043254951.1 4996350..4997222(+) (pilD) [Pseudomonas knackmussii B13]
ATGCCCGCACTTGAATTCCTGGCCAGCCACGCGCTGGCCTTTGTTTTGTGTGCTGCCCTCCTCGGCCTTCTGGTCGGCAG
CTTTCTCAACGTAGTCATCCACCGCCTGCCGAAAATGCTGCAACTCGACTGGGAGCAGCAGGCCAGGGACACGCTCGCTC
TTCCGCATCCCGAAAAGCGCGAAACCTACAACCTCATCCTGCCCAACTCGCATTGCCCGCATTGCGACCATGAGATTCGC
CCCTGGGAGAACATCCCGGTGGTGAGCTACCTGGCGCTTCGCGGCAAGTGCTCTTCCTGCAAGGCGCCGATCAGCGCGCG
TTATCCGTTGATCGAGGTGGCCACCGCCCTCCTCTCTGCTTTCGTTGCCTGGCACTTCGGTTTCACCTGGCAGGCGGGCG
CGATGTTGCTGTTGAGCTGGGGCCTTCTTGCGATGAGCATGATCGATGTCGATCACCAACTGCTGCCCGATGCGCTGGTG
CTTCCGTTGCTCTGGCTCGGTCTGATCGTCAATGCCTTCGGCCTCTTTACCTCGCTGAGCGATGCGCTTTGGGGCGCGGT
TTTCGGCTACCTCAGCCTGTGGACGGTGTTCTGGGTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAGCTGCTGGCCATGCTCGGCGCCTGGGGTGGCTGGCAGGTGCTGCCGCTGACCATCCTGCTCTCCTCGCTGGTTGGG
GCGGTGCTGGGCGTTATCATGCTGCGCCTACGCAATGCCGAGAGCGGCACGCCTATCCCCTTCGGGCCCTACCTGGCCAT
CGCCGGCTGGATTGCGCTGCTCTGGGGTGATCAAATAACCACTTCCTATCTGCAGTTCGCCGGTTTCCGCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.386	98.276	0.534
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	51.773	97.241	0.503
pilD	Acinetobacter nosocomialis M2	51.064	97.241	0.497
pilD	Neisseria gonorrhoeae MS11	50.19	90.69	0.455