Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PKB_RS23340 Genome accession   NZ_HG322950
Coordinates   4993422..4995125 (+) Length   567 a.a.
NCBI ID   WP_043254947.1    Uniprot ID   A0A024HLZ7
Organism   Pseudomonas knackmussii B13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4988422..5000125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PKB_RS23325 (PKB_4739) - 4989263..4990831 (-) 1569 WP_043254942.1 O-antigen ligase family protein -
  PKB_RS23330 (PKB_4740) - 4990844..4992592 (-) 1749 WP_167333376.1 sulfatase-like hydrolase/transferase -
  PKB_RS23335 (PKB_4741) comP 4992790..4993194 (-) 405 WP_043254945.1 pilin Machinery gene
  PKB_RS23340 (PKB_4742) pilB 4993422..4995125 (+) 1704 WP_043254947.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PKB_RS23345 (PKB_4743) pilC 4995129..4996346 (+) 1218 WP_043254948.1 type II secretion system F family protein Machinery gene
  PKB_RS23350 (PKB_4744) pilD 4996350..4997222 (+) 873 WP_043254951.1 A24 family peptidase Machinery gene
  PKB_RS23355 (PKB_4745) coaE 4997308..4997916 (+) 609 WP_043254953.1 dephospho-CoA kinase -
  PKB_RS23360 (PKB_4746) yacG 4997913..4998122 (+) 210 WP_043254955.1 DNA gyrase inhibitor YacG -
  PKB_RS23365 (PKB_4747) - 4998188..4998403 (-) 216 WP_043254956.1 hypothetical protein -
  PKB_RS23370 (PKB_4748) - 4998510..4999217 (-) 708 WP_043254958.1 energy-coupling factor ABC transporter permease -
  PKB_RS23375 (PKB_4749) - 4999214..4999681 (-) 468 WP_242411188.1 FAD/FMN-containing dehydrogenase -
  PKB_RS23380 (PKB_4750) - 4999685..5000101 (-) 417 WP_043254962.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62535.54 Da        Isoelectric Point: 5.4631

>NTDB_id=1111931 PKB_RS23340 WP_043254947.1 4993422..4995125(+) (pilB) [Pseudomonas knackmussii B13]
MNDQVVLSGLARQLVIAELLDEKSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGIAYFDLGSIDRDSIPKD
LVSEKLVRQHRVIPLWRRGNKLFVGLSDPTNHQAVTDVQFSTGLTTEAILVEDDKLGDAIEKLFESPTGGLDSLADVDLE
GLDIESGDAERKEESIGGDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDNQKQLYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGMDFSTALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSVNLIIAQRLARKLCSACKKEHEVPREALLHEGFP
EDKIGTFKLYSPVGCENCNGGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMNGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1111931 PKB_RS23340 WP_043254947.1 4993422..4995125(+) (pilB) [Pseudomonas knackmussii B13]
ATGAACGATCAAGTCGTCCTCTCCGGTCTCGCCCGCCAACTGGTGATTGCCGAGCTGCTCGATGAGAAGAGTGCGCAACA
GGCTCAGCAGCAGGCACAGCGCAACAAGCTATCGCTGGTGACCTATCTGGTGCAGAACAAGCTGGTCAAGAGCCGCCCGC
TGACCGAACTGGCCGCCGACCAGTTCGGCATCGCCTATTTCGATTTGGGCAGTATCGATAGAGATAGCATTCCCAAAGAT
CTGGTCAGCGAAAAGCTGGTGCGACAACACCGGGTCATTCCATTGTGGCGCCGAGGCAACAAGCTGTTCGTTGGCTTGTC
CGACCCCACCAACCACCAGGCCGTCACCGATGTCCAGTTCAGCACGGGCCTGACCACCGAGGCCATACTGGTTGAGGACG
ACAAACTCGGCGATGCCATCGAAAAACTCTTCGAAAGTCCCACAGGAGGGCTAGATTCTCTCGCCGACGTCGACCTGGAA
GGACTGGATATCGAGAGTGGCGACGCAGAGCGTAAGGAAGAGAGCATTGGCGGCGACGCCGACGACGCTCCCGTAGTGCG
CTTCGTCAACAAGATGCTGCTGGATGCCATCCGAGGCGGCTCATCAGACTTGCACTTCGAACCCTACGAAAAGATCTACC
GCGTGCGCTTCCGCACCGACGGCATGCTGCACGAGGTCGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATCTCCGCACGC
TTGAAGGTCATGGCCGGGCTGGACATTTCCGAGCGCCGCAAGCCGCAGGACGGCCGCATCAAGATGCGAGTGTCGAAGAC
CAAGTCCATCGACTTCCGGGTCAACACTCTGCCCACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACTCGGCCA
GCGCGCAAATGGGCATTGACGCATTGGGCTACGAGGACAACCAGAAGCAACTCTATCTGGAGGCGCTGAAACAGCCACAA
GGCATGATCCTGGTAACCGGCCCCACCGGTTCGGGCAAGACGGTATCCCTGTATACCGGCCTGAATATCCTCAATACCCC
GGACATCAACATTTCCACTGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTGAATCCCAAGC
AGGGCATGGATTTCTCCACAGCCTTGCGCGCGTTTCTGCGGCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACCCGCCTGCTGAACATGGGCGTGGCCTCGTTCAACCTGGCTACCTCGGTCAACCTGATCATTGCCCAGC
GTCTGGCACGCAAGTTGTGTTCGGCTTGCAAGAAGGAGCACGAAGTTCCGCGCGAAGCCCTGCTCCATGAAGGCTTCCCG
GAAGACAAGATCGGCACCTTCAAGCTCTACTCGCCAGTGGGGTGTGAAAATTGCAACGGTGGCTACAAGGGCCGCGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATTCAGATCGCCGAAC
AAGCCCGCAAAGAAGGCTTCAATGATCTGCGCACCTCGGGCCTGCTGAAAGCCATGAATGGCATCACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024HLZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

99.824

0.58
  pilB Acinetobacter baumannii D1279779

57.597

99.824

0.575
  pilB Legionella pneumophila strain ERS1305867

55.398

99.647

0.552
  pilF Neisseria gonorrhoeae MS11

50.441

100

0.504
  pilB Vibrio cholerae strain A1552

50.799

99.295

0.504
  pilB Vibrio campbellii strain DS40M4

50

99.824

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

51.565

95.767

0.494
  pilF Thermus thermophilus HB27

38.772

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

93.651

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.358

0.362

Multiple sequence alignment