Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOB85_RS09995 Genome accession   NZ_CP184834
Coordinates   472824..473930 (+) Length   368 a.a.
NCBI ID   WP_005384523.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain HoVa25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467824..478930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOB85_RS09960 (ACOB85_09960) - 467864..468466 (-) 603 WP_005381009.1 XTP/dITP diphosphatase -
  ACOB85_RS09965 (ACOB85_09965) - 468585..469016 (-) 432 WP_005381010.1 DUF4426 domain-containing protein -
  ACOB85_RS09970 (ACOB85_09970) yggU 469145..469435 (-) 291 WP_005381011.1 DUF167 family protein YggU -
  ACOB85_RS09975 (ACOB85_09975) - 469435..469992 (-) 558 WP_005381012.1 YggT family protein -
  ACOB85_RS09980 (ACOB85_09980) proC 470045..470863 (-) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  ACOB85_RS09985 (ACOB85_09985) - 471023..471724 (-) 702 WP_042521292.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOB85_RS09990 (ACOB85_09990) pilT 471753..472793 (+) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOB85_RS09995 (ACOB85_09995) pilU 472824..473930 (+) 1107 WP_005384523.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOB85_RS10000 (ACOB85_10000) ruvX 473993..474418 (-) 426 WP_053304143.1 Holliday junction resolvase RuvX -
  ACOB85_RS10005 (ACOB85_10005) - 474454..475017 (-) 564 WP_005381020.1 YqgE/AlgH family protein -
  ACOB85_RS10010 (ACOB85_10010) gshB 475145..476095 (-) 951 WP_285385469.1 glutathione synthase -
  ACOB85_RS10015 (ACOB85_10015) rsmE 476109..476840 (-) 732 WP_017821890.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACOB85_RS10020 (ACOB85_10020) endA 476954..477649 (-) 696 WP_005381023.1 deoxyribonuclease I -
  ACOB85_RS10025 (ACOB85_10025) - 477800..478297 (-) 498 WP_005381025.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41224.33 Da        Isoelectric Point: 6.5943

>NTDB_id=1111373 ACOB85_RS09995 WP_005384523.1 472824..473930(+) (pilU) [Vibrio alginolyticus strain HoVa25]
MDLDKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTEGDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1111373 ACOB85_RS09995 WP_005384523.1 472824..473930(+) (pilU) [Vibrio alginolyticus strain HoVa25]
ATGGATCTAGATAAATTTCTCGAAGGCATGCTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTCACCGAGGGCGATGTGGCTGCATTACTCGACAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCGAATTTTGCCATTGTGAGAGACTGTGGCCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACTAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCAATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGTTCGGGTA
AATCGACCACTATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAGTTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGTGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAACAATGCAAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGCTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGTCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGCGCCGACTCGGCGAATGATCTGCGCTTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.923

88.315

0.397
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Acinetobacter baylyi ADP1

41.39

89.946

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361

Multiple sequence alignment