Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACKTVB_RS19580 Genome accession   NZ_AP038752
Coordinates   4042242..4043222 (-) Length   326 a.a.
NCBI ID   WP_407859018.1    Uniprot ID   -
Organism   Enterobacter pseudoroggenkampii strain GVv1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4037242..4048222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKTVB_RS19550 (GVv1_38590) - 4038118..4038615 (+) 498 WP_032650144.1 SprT family zinc-dependent metalloprotease -
  ACKTVB_RS19555 (GVv1_38600) endA 4038710..4039417 (+) 708 WP_023333343.1 deoxyribonuclease I -
  ACKTVB_RS19560 (GVv1_38610) rsmE 4039469..4040200 (+) 732 WP_024906558.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACKTVB_RS19565 (GVv1_38620) gshB 4040220..4041167 (+) 948 WP_061714498.1 glutathione synthase -
  ACKTVB_RS19570 (GVv1_38630) - 4041255..4041815 (+) 561 WP_032645077.1 YqgE/AlgH family protein -
  ACKTVB_RS19575 (GVv1_38640) ruvX 4041815..4042231 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  ACKTVB_RS19580 (GVv1_38650) pilT 4042242..4043222 (-) 981 WP_407859018.1 type IV pilus twitching motility protein PilT Machinery gene
  ACKTVB_RS19585 (GVv1_38660) - 4043240..4043941 (+) 702 WP_248272396.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACKTVB_RS19590 (GVv1_38670) - 4043963..4044529 (+) 567 WP_090418613.1 YggT family protein -
  ACKTVB_RS19595 (GVv1_38680) yggU 4044526..4044822 (+) 297 WP_023333349.1 DUF167 family protein YggU -
  ACKTVB_RS19600 (GVv1_38690) - 4044826..4045419 (+) 594 WP_048991669.1 XTP/dITP diphosphatase -
  ACKTVB_RS19605 (GVv1_38700) hemW 4045412..4046554 (+) 1143 WP_024906552.1 radical SAM family heme chaperone HemW -
  ACKTVB_RS19610 (GVv1_38710) - 4046618..4046980 (+) 363 WP_407859019.1 endonuclease domain-containing protein -
  ACKTVB_RS19615 (GVv1_38720) - 4047042..4047756 (-) 715 Protein_3839 DUF2884 domain-containing protein -
  ACKTVB_RS19620 (GVv1_38740) - 4047813..4048139 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35452.55 Da        Isoelectric Point: 6.8407

>NTDB_id=111062 ACKTVB_RS19580 WP_407859018.1 4042242..4043222(-) (pilT) [Enterobacter pseudoroggenkampii strain GVv1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKAWLNDEQQGIWWAIGQVDFAVTVAGGQRLRG
SAFKQMSGISVTLRLLPRTCPQLASLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNRHADGHILTLEDPVE
FIYRSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=111062 ACKTVB_RS19580 WP_407859018.1 4042242..4043222(-) (pilT) [Enterobacter pseudoroggenkampii strain GVv1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCTGACGTCGGGGCGTTATTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGGATTTGGTGGGCCATCGGGCAGGTGGATTTTGCCGTGACGGTGGCGGGAGGCCAGCGTCTGCGCGGC
AGTGCCTTTAAGCAGATGAGCGGTATCTCTGTAACGCTCCGGCTGTTGCCGCGTACCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGCAATCCCGGAACTGTTGTCCAATGACGCCGGACTGATTCTGGTGACAGGAGCAACCGGCAGCGGTAAAT
CAACTACGCTGGCGGCGATGGTCGATTTTCTTAACCGCCATGCTGATGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCGGAGCGAGCGTTGTCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAAGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTGATTCTGCTTGGAGAGTTGCGCGACAGCGAAACGATCCGCCTTGCGCTAACTG
CGGCGGAAACCGGGCATCTGGTGCTGGCGACCCTGCATACGCGCGGAGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGCTGTATGAACTGCTGGTGAACACCGCAGCGGCGGCGAATCTCATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATTATTCAAACCGGTCAGCAGGCAGGTATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment