Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN079_RS08120 Genome accession   NZ_CP184478
Coordinates   1799134..1800168 (+) Length   344 a.a.
NCBI ID   WP_420234283.1    Uniprot ID   -
Organism   Pseudomonas sp. ABY48     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1794134..1805168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN079_RS08090 (ACN079_08090) metW 1794562..1795182 (-) 621 WP_420234277.1 methionine biosynthesis protein MetW -
  ACN079_RS08095 (ACN079_08095) metX 1795190..1796329 (-) 1140 WP_420234278.1 homoserine O-succinyltransferase MetX -
  ACN079_RS08100 (ACN079_08100) - 1796640..1796933 (-) 294 WP_420234279.1 DUF167 domain-containing protein -
  ACN079_RS08105 (ACN079_08105) - 1796939..1797529 (-) 591 WP_420234280.1 YggT family protein -
  ACN079_RS08110 (ACN079_08110) proC 1797540..1798358 (-) 819 WP_420234281.1 pyrroline-5-carboxylate reductase -
  ACN079_RS08115 (ACN079_08115) - 1798394..1799080 (-) 687 WP_420234282.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN079_RS08120 (ACN079_08120) pilT 1799134..1800168 (+) 1035 WP_420234283.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN079_RS08125 (ACN079_08125) - 1800328..1800927 (-) 600 WP_420234284.1 C40 family peptidase -
  ACN079_RS08130 (ACN079_08130) - 1801177..1801605 (+) 429 WP_057449544.1 NINE protein -
  ACN079_RS08135 (ACN079_08135) - 1801738..1803009 (-) 1272 WP_420234285.1 dihydroorotase -
  ACN079_RS08140 (ACN079_08140) - 1803006..1804010 (-) 1005 WP_420234286.1 aspartate carbamoyltransferase catalytic subunit -
  ACN079_RS08145 (ACN079_08145) pyrR 1804035..1804541 (-) 507 WP_420234287.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACN079_RS08150 (ACN079_08150) ruvX 1804627..1805064 (-) 438 WP_003177497.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38134.75 Da        Isoelectric Point: 6.9777

>NTDB_id=1108413 ACN079_RS08120 WP_420234283.1 1799134..1800168(+) (pilT) [Pseudomonas sp. ABY48]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDYLNSHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVNKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1108413 ACN079_RS08120 WP_420234283.1 1799134..1800168(+) (pilT) [Pseudomonas sp. ABY48]
ATGGATATCACTGAGTTACTGGCATTCAGCGCCAAGCAGGGCGCGTCGGACCTGCACCTGTCCGCCGGATTGCCGCCGAT
GATCCGCGTCGATGGCGATGTCAGGCGCATCAACCTGCCGGCATTGGACCACAAGCAGGTCCATGAACTGATCTACGACA
TCATGAACGACCGGCAACGCGTAGATTACGAAAAGTTTCTGGAGACTGATTTTTCCTTCGACGTGCCGGGCGTCGCGCGG
TTCCGGGTCAACGCCTTCAACCAGAATCGCGGTGCAGGGGCAGTGTTTCGGACCATTCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGGATGGGCGAGGTGTTTCGCAAAGTGACCGAGGCGCCTCGCGGACTGGTGTTGGTGACCGGGCCGACCGGTT
CAGGCAAGTCCACCACCCTGGCGGCCATGATCGACTACTTGAACAGCCACAAGCACCATCACATCCTCACCATCGAAGAC
CCTATCGAATTCGTTCACGAACCGCGCAAATGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCAGGGTTTCTCCAC
GGCCCTGCGTTCGGCCCTGCGGGAAGACCCGGACGTGATCCTGGTGGGGGAGATGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACGGCCGCGGAAACCGGGCACCTGGTGTTCGGCACCCTGCACACCACGTCGGCAGCCAAGACCATTGACCGGGTG
GTGGACGTGTTCCCCGGGGATGAGAAGTCCATGGTTCGCTCGATGTTGTCGGAGTCGTTGCAGGCGGTGATCTCCCAGAC
GCTGGTCAAGAAGATCGGCGGCGGCCGGGTCGCGGCGCATGAAATCATGCTGGGCACCTCGGCTATTCGGAACCTGATCC
GTGAAGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCGGTTCGCTGGGGATGCAGACGCTGGACATGTGCCTG
AAGGACTTGGTGAACAAGGGGCTGATCAGTCGTGAGCATGCCCGCGAGCGGGCGCGTACGCCGGATAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

88.663

100

0.887
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment