Detailed information NTG1108123

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ACOIYJ_RS11415
• Genome accession: NZ_CP184271
• Coordinates: 2493784..2494800 (+)
• Length: 338 a.a.
• NCBI ID: WP_180741628.1
• Uniprot ID: A0A7V8PKC5
• Organism: Pectobacterium brasiliense strain CSR2
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2488784..2499800

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACOIYJ_RS11385	hemW	2489458..2490600 (-)	1143	WP_420064129.1	radical SAM family heme chaperone HemW	-
ACOIYJ_RS11390	rdgB	2490593..2491186 (-)	594	WP_420064128.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
ACOIYJ_RS11395	yggU	2491232..2491522 (-)	291	WP_010295005.1	DUF167 family protein YggU	-
ACOIYJ_RS11400	-	2491519..2492073 (-)	555	WP_010295003.1	YggT family protein	-
ACOIYJ_RS11405	proC	2492123..2492944 (-)	822	WP_420064127.1	pyrroline-5-carboxylate reductase	-
ACOIYJ_RS11410	-	2493035..2493763 (-)	729	WP_420064126.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACOIYJ_RS11415	pilT	2493784..2494800 (+)	1017	WP_180741628.1	type IV pilus twitching motility protein PilT	Machinery gene
ACOIYJ_RS11420	-	2494769..2496595 (-)	1827	WP_420064125.1	diguanylate cyclase	-
ACOIYJ_RS11425	-	2497120..2498691 (-)	1572	WP_180741630.1	methyl-accepting chemotaxis protein	-
ACOIYJ_RS11430	nsrR	2499019..2499444 (+)	426	WP_420064124.1	nitric oxide-sensing transcriptional repressor NsrR	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 36900.29 Da
• Isoelectric Point: 6.8718

>NTDB_id=1108123 ACOIYJ_RS11415 WP_180741628.1 2493784..2494800(+) (pilT) [Pectobacterium brasiliense strain CSR2]
MELDEWMVRSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCDAWLEPAQREQLRQFGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLDGLHAPPVLSMLLEKPNGLILITGATGSGKSTTLAAMLGALNDRSDRHVITLE
DPIEFIHVSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVGNLIREGKTHQLPGLIQTGTAAGMQTFEQS
YQQRCRDGLISHSCALAV

Nucleotide

• Length: 1017 bp

>NTDB_id=1108123 ACOIYJ_RS11415 WP_180741628.1 2493784..2494800(+) (pilT) [Pectobacterium brasiliense strain CSR2]
ATGGAATTGGATGAGTGGATGGTGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGCCACCCGCCAGT
GTTACGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCGCAGCGAGAGCAACTGCGGCAGTTTGGTCAGGTGGACGGCGCGTTAATGCTGCCCGATGGTCAA
CGTCTGCGGGTGAATGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCACTGCGTATTATCCCGTCCGCTCAGCCGTCATT
GGATGGGCTACACGCGCCCCCCGTTCTGTCGATGCTGCTGGAGAAACCGAACGGACTGATCCTGATTACTGGTGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACCGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGTCAGCCGGCGTTGCCTGATTCAACAGCGAGAGATCGGTGCGCACAGCGCGTCTTTTGC
GCAGGCGTTACGGGCGGCATTGCGGGAAGATCCCGATGTGATTCTGCTGGGGGAACTTCGTGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGCTATCCACGCTGCATACGCGCAGCGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAAAAAGCCTATGTTCGCAGCCAGCTCGCCACCTGCTTGCAGGCTGTGGTGACGCA
AAGGCTGTTGCCCGCAGCGCAAGGCGGTCGCATTGCACTTTATGAAGTGCTAACGGCGACGGCAGCAGTCGGCAACCTGA
TTCGGGAAGGGAAAACGCATCAGCTTCCTGGCCTGATTCAAACGGGAACGGCGGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (10-270)

3D structure

• AlphaFold DB: A0A7V8PKC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Neisseria meningitidis 8013	50	99.408	0.497
pilT	Vibrio cholerae strain A1552	50.909	97.633	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	50.909	97.633	0.497
pilT	Neisseria gonorrhoeae MS11	49.702	99.408	0.494
pilT	Pseudomonas stutzeri DSM 10701	48.955	99.112	0.485
pilT	Pseudomonas aeruginosa PAK	48.657	99.112	0.482
pilT	Acinetobacter baylyi ADP1	48.225	100	0.482
pilT	Legionella pneumophila strain ERS1305867	48.358	99.112	0.479
pilT	Legionella pneumophila strain Lp02	48.358	99.112	0.479
pilT	Acinetobacter nosocomialis M2	47.761	99.112	0.473
pilT	Acinetobacter baumannii D1279779	47.761	99.112	0.473
pilT	Acinetobacter baumannii strain A118	47.761	99.112	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.84	99.704	0.417
pilU	Pseudomonas stutzeri DSM 10701	40.653	99.704	0.405
pilU	Vibrio cholerae strain A1552	39.169	99.704	0.391
pilU	Acinetobacter baylyi ADP1	37.87	100	0.379
pilB	Vibrio cholerae strain A1552	31.865	100	0.364