Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGJAX_RS04445 Genome accession   NZ_AP035776
Coordinates   933182..934216 (-) Length   344 a.a.
NCBI ID   WP_247254447.1    Uniprot ID   -
Organism   Pseudomonas moorei strain m318     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 928182..939216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGJAX_RS04415 (PMm318_A08560) ruvX 928313..928750 (+) 438 WP_090326077.1 Holliday junction resolvase RuvX -
  ACGJAX_RS04420 (PMm318_A08570) pyrR 928845..929348 (+) 504 WP_090326079.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACGJAX_RS04425 (PMm318_A08580) - 929376..930380 (+) 1005 WP_007939362.1 aspartate carbamoyltransferase catalytic subunit -
  ACGJAX_RS04430 (PMm318_A08590) - 930377..931648 (+) 1272 WP_248914474.1 dihydroorotase -
  ACGJAX_RS04435 (PMm318_A08600) - 931749..932168 (-) 420 WP_090326084.1 TM2 domain-containing protein -
  ACGJAX_RS04440 (PMm318_A08610) - 932419..933039 (+) 621 WP_390957550.1 NlpC/P60 family protein -
  ACGJAX_RS04445 (PMm318_A08620) pilT 933182..934216 (-) 1035 WP_247254447.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGJAX_RS04450 (PMm318_A08630) - 934274..934960 (+) 687 WP_090326092.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGJAX_RS04455 (PMm318_A08640) proC 934995..935813 (+) 819 WP_390957552.1 pyrroline-5-carboxylate reductase -
  ACGJAX_RS04460 (PMm318_A08650) - 935838..936425 (+) 588 WP_090326098.1 YggT family protein -
  ACGJAX_RS04465 (PMm318_A08660) - 936609..937748 (+) 1140 WP_090326101.1 homoserine O-acetyltransferase -
  ACGJAX_RS04470 (PMm318_A08670) metW 937756..938376 (+) 621 WP_390957555.1 methionine biosynthesis protein MetW -
  ACGJAX_RS04475 (PMm318_A08680) - 938403..938837 (+) 435 WP_090326107.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38039.62 Da        Isoelectric Point: 6.9825

>NTDB_id=110773 ACGJAX_RS04445 WP_247254447.1 933182..934216(-) (pilT) [Pseudomonas moorei strain m318]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKEVQALIYDIMNDTQRVDFEKHLETDFSFELPGVAR
FRVNAFNQSRGAGAVFRTIPSRVLSMEELGMGDVFRKMTDAPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PVEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTMHTTSAAKTIDRI
IDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRTAAHEIMLGTSAIRNLIREDKVAQMYSSIQAGGSLGMQTLDMSL
KDLVTKGLVTRDHAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=110773 ACGJAX_RS04445 WP_247254447.1 933182..934216(-) (pilT) [Pseudomonas moorei strain m318]
ATGGATATCACTGAGCTGCTGGCGTTCAGCGCCAAACAAGGAGCATCCGACCTGCACCTGTCCGCGGGCCTGCCACCGAT
GATTCGGGTTGATGGCGATGTGCGGCGCATCAACCTGCCGCCTCTGGACCACAAGGAAGTGCAGGCGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAGCACCTGGAAACCGACTTTTCCTTTGAATTGCCCGGCGTGGCACGT
TTTAGGGTCAACGCGTTCAACCAGAGCCGCGGTGCTGGCGCCGTGTTTCGCACCATTCCCTCCAGGGTCTTGAGCATGGA
GGAGTTGGGCATGGGTGATGTGTTTCGCAAAATGACCGACGCTCCCCGTGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CCGGCAAGTCCACGACGCTGGCGGCGATGATCGATTATCTGAACAATCATCGCCACCACCATATCCTCACTATTGAAGAC
CCTGTCGAGTTCGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGTGAGGTCCATCGTGATACCCGCAGCTTTGCCAC
GGCCTTGCGTTCGGCCCTGCGCGAAGACCCGGATGTCATTCTGGTCGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACCGCCGCCGAAACCGGTCATCTGGTGTTCGGCACGATGCACACCACCTCGGCGGCGAAGACCATTGACCGGATC
ATCGACGTGTTTCCGGGGGATGAAAAGTCGATGGTGCGTTCGATGCTGTCCGAATCGTTGCTGGCAGTGGTTTCCCAAAC
CCTGGTCAAGAAGATCGGCGGCGGGCGTACAGCCGCCCACGAGATCATGCTCGGCACGTCGGCGATCCGAAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCATCGATTCAGGCAGGAGGGTCGCTGGGCATGCAGACGCTGGACATGAGCCTG
AAGGACCTGGTGACCAAGGGCCTGGTCACTCGCGACCATGCGCGGGAGAAGGCGCGGTCGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.302

100

0.843
  pilT Pseudomonas aeruginosa PAK

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter baumannii strain A118

76.163

100

0.762
  pilT Acinetobacter nosocomialis M2

76.163

100

0.762
  pilT Acinetobacter baumannii D1279779

76.163

100

0.762
  pilT Legionella pneumophila strain Lp02

71.512

100

0.715
  pilT Legionella pneumophila strain ERS1305867

71.512

100

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.656

97.965

0.663
  pilT Vibrio cholerae strain A1552

67.656

97.965

0.663
  pilT Neisseria meningitidis 8013

65.507

100

0.657
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment