Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLKM2_RS03250 Genome accession   NZ_CP183899
Coordinates   702634..703755 (-) Length   373 a.a.
NCBI ID   WP_074384137.1    Uniprot ID   -
Organism   Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697634..708755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM2_RS03220 - 698379..699227 (+) 849 WP_005096047.1 class II glutamine amidotransferase -
  ACLKM2_RS03225 - 699509..699964 (+) 456 WP_005096048.1 bacteriohemerythrin -
  ACLKM2_RS03230 - 700108..701187 (+) 1080 WP_005096049.1 hypothetical protein -
  ACLKM2_RS03235 - 701187..701519 (+) 333 WP_100328916.1 RnfH family protein -
  ACLKM2_RS03240 - 701620..702018 (-) 399 WP_004279275.1 outer membrane protein assembly factor BamE -
  ACLKM2_RS03245 fur 702131..702568 (+) 438 WP_005096051.1 ferric iron uptake transcriptional regulator -
  ACLKM2_RS03250 pilU 702634..703755 (-) 1122 WP_074384137.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLKM2_RS03255 pilT 703786..704823 (-) 1038 WP_004279279.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLKM2_RS03260 - 704947..705633 (+) 687 WP_074384176.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLKM2_RS03265 - 705667..706770 (-) 1104 WP_180040353.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41654.92 Da        Isoelectric Point: 6.9358

>NTDB_id=1107041 ACLKM2_RS03250 WP_074384137.1 702634..703755(-) (pilU) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
MDFNGLLDYMVEKKASDLFITADVEPSIKINGQILPIGSIKLPGAAVGQLLHSIMSDKQRKEFADTRECNFAISTPDKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLVGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNMKAIVAQQLIPTIDGNGRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLAEEGGSHLLRAHSNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1107041 ACLKM2_RS03250 WP_074384137.1 702634..703755(-) (pilU) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
ATGGATTTTAACGGCTTACTCGATTATATGGTGGAGAAAAAAGCATCGGATCTGTTCATTACAGCCGATGTCGAACCTTC
AATCAAGATTAACGGGCAGATTTTACCGATTGGTTCGATAAAATTACCCGGCGCGGCAGTAGGACAACTGCTCCATTCCA
TCATGAGCGACAAGCAGCGTAAAGAATTTGCAGACACGCGTGAATGTAACTTCGCCATCAGCACACCGGACAAAAGTGCC
CGCTTCCGTGTCAGTGCCTTTCAGCAGCGTGATCAGCCGGGTATGGTGTTGCGTCGGATTGAAACCGTGATTCCGACCAT
GGATGAACTCAAATTACCGCCGATTCTAAAAGAACTGGCGATGACCAAACGCGGCATCATTATTTTTGTCGGGGCAACCG
GTACCGGTAAATCAACCTCGCTGGCCTCGCTGGTCGGTTATCGCAATGAAAATTCCAAAGGTCATATCATTACTATTGAA
GATCCGATCGAATTCGTTCACCAGCATAAAGGCTGTATCATCACCCAGCGTGAAGTCGGGATTGATACCGATTCCTTTGA
AATTGCCCTGAAAAACACCTTACGTCAGGCGCCGGACGTGATTCTGATTGGTGAGATTCGTTCGCGTGAAACCATGGATT
ATGCGATTGCCTTTGCCGAAACCGGCCATCTGGTCTTTGCGACCCTGCATGCCAACAACGCCAACCAGGCCATTGACCGG
ATCATTCATTTCTTTGAAGCAGACCGGCATAACCAGCTGTTCATGGATTTGTCGCTGAACATGAAAGCCATTGTGGCACA
GCAACTGATCCCAACCATCGATGGCAATGGTCGTCGTGCCGCGATTGAGATTCTGATTAACTCCCCCCTGATTGCTGACC
TGATCCGTAAAGGTGATGTGCATGAGATCAAGGACCTGATGAAGCGTTCCCGTGAACTGGGCATGCAAACCTTCGATCAG
GCACTGTATGACCTGTATAAGGCCAAGCAGATCAGCTATAAAGATGCGCTGAAACATGCGGATTCACCCAATGACTTACG
TCTGCAAATCAAACTGGCAGAAGAAGGCGGCAGTCATTTATTACGTGCACATTCCAATATCACCTTTGATGGCCAGTCCT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

71.693

100

0.727
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.464

0.678
  pilU Vibrio cholerae strain A1552

58.405

94.102

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Acinetobacter baumannii strain A118

42.274

91.957

0.389
  pilT Acinetobacter nosocomialis M2

42.274

91.957

0.389
  pilT Acinetobacter baumannii D1279779

42.274

91.957

0.389
  pilT Legionella pneumophila strain Lp02

42.857

90.08

0.386
  pilT Legionella pneumophila strain ERS1305867

42.857

90.08

0.386
  pilT Pseudomonas aeruginosa PAK

41.04

92.761

0.381
  pilT Vibrio cholerae strain A1552

40.171

94.102

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.171

94.102

0.378
  pilT Acinetobacter baylyi ADP1

40.816

91.957

0.375
  pilT Neisseria gonorrhoeae MS11

40.173

92.761

0.373
  pilT Neisseria meningitidis 8013

40.173

92.761

0.373
  pilT Pseudomonas stutzeri DSM 10701

39.595

92.761

0.367

Multiple sequence alignment