Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   strainIA91_RS05095 Genome accession   NZ_AP035449
Coordinates   1169056..1170120 (+) Length   354 a.a.
NCBI ID   WP_400168048.1    Uniprot ID   -
Organism   Fidelibacter multiformis strain IA91     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1164056..1175120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  strainIA91_RS05080 (FMIA91_09880) pilO 1166105..1166671 (+) 567 WP_400168045.1 type 4a pilus biogenesis protein PilO -
  strainIA91_RS05085 (FMIA91_09890) - 1166673..1167200 (+) 528 WP_400168046.1 hypothetical protein -
  strainIA91_RS05090 (FMIA91_09900) - 1167275..1169038 (+) 1764 WP_400168047.1 hypothetical protein -
  strainIA91_RS05095 (FMIA91_09910) pilT 1169056..1170120 (+) 1065 WP_400168048.1 type IV pilus twitching motility protein PilT Machinery gene
  strainIA91_RS05100 (FMIA91_09920) - 1170122..1171225 (+) 1104 WP_400168049.1 PilT/PilU family type 4a pilus ATPase -
  strainIA91_RS05105 (FMIA91_09930) - 1171250..1173178 (+) 1929 WP_400168050.1 sensor histidine kinase -
  strainIA91_RS05110 (FMIA91_09940) - 1173329..1173700 (+) 372 WP_400168051.1 response regulator transcription factor -
  strainIA91_RS05115 (FMIA91_09950) - 1173697..1174476 (+) 780 WP_400168052.1 prepilin peptidase -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38764.43 Da        Isoelectric Point: 6.1718

>NTDB_id=110696 strainIA91_RS05095 WP_400168048.1 1169056..1170120(+) (pilT) [Fidelibacter multiformis strain IA91]
MATEIYTLLKFAVDNGASDLHLCVGSPPIVRVDGDMLRTNLDPMDSTTIHSLVYDVMSDEQRKLFETNWEIDFSREFKGI
GRFRVNVYRDINGEAAVFRMIPTKVLSFKELGLPDVLLNIASQDKGLVLVTGPTGSGKSTTLATIIDFINKKYKKHIITV
EDPVEFVHVSDESLVNQREVGFNTKSFAAALRSALREDPDVILVGEMRDLETTSLAITAAETGHLVFGTLHTNNAAKTID
RVIDQYPATQQAQIRSMLSESLLAVVSQVLLKHASGRGRVAAFEIMIGTSGIRNLIRESKVFQIPSAIQTGSNVGMVSLD
QSLVRLAEAGKIDTNIAIQSATNPGYVKKNLGLE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=110696 strainIA91_RS05095 WP_400168048.1 1169056..1170120(+) (pilT) [Fidelibacter multiformis strain IA91]
ATGGCGACGGAAATCTATACACTCTTAAAATTTGCAGTCGATAACGGAGCATCGGACCTTCATCTTTGTGTGGGTTCGCC
CCCCATTGTCCGGGTGGACGGCGATATGCTCCGGACCAATCTGGATCCTATGGACAGTACCACGATTCATTCCCTTGTCT
ACGATGTGATGAGTGATGAACAACGGAAACTCTTTGAAACAAACTGGGAAATCGATTTCTCCCGGGAATTCAAAGGCATC
GGACGGTTCAGGGTGAATGTCTACAGGGATATCAACGGCGAAGCGGCTGTTTTCCGGATGATCCCCACAAAGGTTTTAAG
CTTTAAAGAACTGGGACTCCCGGATGTTCTTCTCAATATTGCATCCCAGGATAAAGGCCTTGTGCTGGTCACCGGTCCTA
CAGGGAGTGGAAAATCCACAACTCTGGCTACGATCATTGATTTTATTAATAAAAAGTATAAAAAACATATTATTACGGTT
GAAGATCCCGTAGAGTTTGTCCATGTATCCGATGAATCACTGGTGAATCAGCGGGAAGTCGGATTCAACACCAAATCCTT
TGCGGCAGCTTTGAGGAGCGCCCTCCGTGAAGATCCCGATGTGATTCTGGTTGGGGAGATGAGGGATCTGGAAACCACCA
GTCTGGCCATCACCGCGGCAGAAACAGGTCACCTGGTGTTTGGAACGCTTCACACCAATAACGCCGCAAAGACCATCGAC
CGGGTCATCGACCAGTATCCGGCCACACAGCAGGCACAAATCCGCTCCATGTTATCGGAATCCCTCCTGGCCGTTGTCTC
ACAGGTTTTACTGAAACATGCCTCCGGCCGGGGTCGGGTAGCTGCCTTTGAGATTATGATCGGAACCAGCGGAATCCGGA
ATCTGATCCGGGAAAGCAAGGTGTTTCAGATTCCTTCGGCCATTCAAACCGGCTCCAATGTGGGGATGGTGTCTCTCGAC
CAGTCCCTGGTCCGGCTGGCGGAAGCCGGTAAAATCGATACCAATATCGCCATCCAGAGCGCGACCAATCCCGGTTATGT
GAAGAAAAATCTGGGCTTAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

59.531

96.328

0.573
  pilT Acinetobacter baumannii D1279779

59.531

96.328

0.573
  pilT Acinetobacter baumannii strain A118

59.531

96.328

0.573
  pilT Pseudomonas stutzeri DSM 10701

59.531

96.328

0.573
  pilT Pseudomonas aeruginosa PAK

59.238

96.328

0.571
  pilT Acinetobacter baylyi ADP1

58.944

96.328

0.568
  pilT Legionella pneumophila strain Lp02

58.235

96.045

0.559
  pilT Legionella pneumophila strain ERS1305867

58.235

96.045

0.559
  pilT Neisseria meningitidis 8013

56.765

96.045

0.545
  pilT Neisseria gonorrhoeae MS11

56.471

96.045

0.542
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

93.22

0.511
  pilT Vibrio cholerae strain A1552

54.848

93.22

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.974

96.328

0.472
  pilU Pseudomonas stutzeri DSM 10701

42.249

92.938

0.393
  pilU Vibrio cholerae strain A1552

40

96.045

0.384
  pilU Acinetobacter baylyi ADP1

36.676

98.588

0.362

Multiple sequence alignment