Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLKM5_RS10895 Genome accession   NZ_CP183893
Coordinates   2277407..2278444 (+) Length   345 a.a.
NCBI ID   WP_005179768.1    Uniprot ID   N9MH18
Organism   Acinetobacter indicus strain D10_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2269941..2331983 2277407..2278444 within 0


Gene organization within MGE regions


Location: 2269941..2331983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM5_RS10875 - 2270585..2271835 (+) 1251 WP_075175173.1 exonuclease SbcCD subunit D C-terminal domain-containing protein -
  ACLKM5_RS10880 - 2271845..2275450 (+) 3606 WP_411687949.1 AAA family ATPase -
  ACLKM5_RS10885 - 2275454..2276551 (+) 1098 WP_180006899.1 acyltransferase family protein -
  ACLKM5_RS10890 - 2276597..2277283 (-) 687 WP_286409321.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLKM5_RS10895 pilT 2277407..2278444 (+) 1038 WP_005179768.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLKM5_RS10900 pilU 2278472..2279590 (+) 1119 WP_005179770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLKM5_RS10905 fur 2279663..2280100 (-) 438 WP_005179771.1 ferric iron uptake transcriptional regulator -
  ACLKM5_RS10910 - 2280213..2280611 (+) 399 WP_005179773.1 outer membrane protein assembly factor BamE -
  ACLKM5_RS10915 - 2280654..2280977 (-) 324 WP_005179774.1 RnfH family protein -
  ACLKM5_RS10920 - 2280977..2282059 (-) 1083 WP_127800517.1 hypothetical protein -
  ACLKM5_RS10925 - 2282226..2282681 (-) 456 WP_168399274.1 bacteriohemerythrin -
  ACLKM5_RS10930 - 2282938..2283786 (-) 849 WP_411687950.1 class II glutamine amidotransferase -
  ACLKM5_RS10935 - 2283873..2284685 (-) 813 WP_286414174.1 GNAT family N-acetyltransferase -
  ACLKM5_RS10940 argB 2284800..2285702 (-) 903 WP_016659457.1 acetylglutamate kinase -
  ACLKM5_RS10945 - 2285713..2287137 (-) 1425 WP_104488139.1 phosphomannomutase/phosphoglucomutase -
  ACLKM5_RS10950 dut 2287321..2287773 (-) 453 WP_045796105.1 dUTP diphosphatase -
  ACLKM5_RS10955 - 2287805..2289844 (-) 2040 WP_005179787.1 UvrD-helicase domain-containing protein -
  ACLKM5_RS10960 - 2290001..2291388 (+) 1388 WP_419867759.1 IS3 family transposase -
  ACLKM5_RS10965 - 2291450..2292100 (-) 651 WP_005179792.1 OmpA family protein -
  ACLKM5_RS10970 - 2292343..2293263 (+) 921 WP_005179793.1 acyltransferase -
  ACLKM5_RS10975 - 2293282..2294337 (+) 1056 WP_180006904.1 preprotein translocase subunit SecA -
  ACLKM5_RS10980 minC 2294459..2295181 (+) 723 WP_005179806.1 septum site-determining protein MinC -
  ACLKM5_RS10985 minD 2295250..2296062 (+) 813 WP_005179808.1 septum site-determining protein MinD -
  ACLKM5_RS10990 minE 2296065..2296337 (+) 273 WP_005179812.1 cell division topological specificity factor MinE -
  ACLKM5_RS10995 - 2296540..2296827 (+) 288 WP_005179823.1 PA4642 family protein -
  ACLKM5_RS11000 - 2296891..2299950 (-) 3060 WP_411687987.1 DNA translocase FtsK -
  ACLKM5_RS11005 trxB 2300198..2301154 (+) 957 WP_016659465.1 thioredoxin-disulfide reductase -
  ACLKM5_RS11010 aat 2301235..2301966 (+) 732 WP_411687988.1 leucyl/phenylalanyl-tRNA--protein transferase -
  ACLKM5_RS11015 - 2301983..2302798 (+) 816 WP_005179833.1 arginyltransferase -
  ACLKM5_RS11020 rimI 2302818..2303282 (-) 465 WP_286390415.1 ribosomal protein S18-alanine N-acetyltransferase -
  ACLKM5_RS11025 - 2303386..2304267 (-) 882 WP_045796116.1 metal-dependent hydrolase -
  ACLKM5_RS11030 tuf 2304480..2305670 (-) 1191 WP_160241040.1 elongation factor Tu -
  ACLKM5_RS11035 fusA 2305765..2307900 (-) 2136 WP_160241039.1 elongation factor G -
  ACLKM5_RS11040 rpsG 2308033..2308503 (-) 471 WP_005179845.1 30S ribosomal protein S7 -
  ACLKM5_RS11045 rpsL 2308683..2309057 (-) 375 WP_002050319.1 30S ribosomal protein S12 -
  ACLKM5_RS11050 - 2309221..2310672 (-) 1452 WP_411687786.1 EcsC family protein -
  ACLKM5_RS11055 - 2310949..2312178 (+) 1230 WP_005179853.1 beta-ketoacyl synthase N-terminal-like domain-containing protein -
  ACLKM5_RS11060 - 2312198..2312650 (+) 453 WP_005179856.1 CopD family protein -
  ACLKM5_RS11065 - 2312706..2314169 (-) 1464 WP_016659472.1 amino acid permease -
  ACLKM5_RS11070 - 2314318..2314680 (-) 363 WP_005179861.1 RidA family protein -
  ACLKM5_RS11075 alr 2314705..2315817 (-) 1113 WP_411687785.1 alanine racemase -
  ACLKM5_RS11080 - 2315840..2317093 (-) 1254 WP_075167990.1 D-amino acid dehydrogenase -
  ACLKM5_RS11085 - 2317233..2317700 (+) 468 WP_005179867.1 Lrp/AsnC ligand binding domain-containing protein -
  ACLKM5_RS11090 - 2317799..2318479 (-) 681 WP_045795528.1 HAD-IA family hydrolase -
  ACLKM5_RS11095 - 2318572..2319339 (+) 768 WP_016659477.1 hydroxymethylpyrimidine/phosphomethylpyrimidine kinase -
  ACLKM5_RS11100 - 2319419..2320513 (-) 1095 WP_168400278.1 catalase family protein -
  ACLKM5_RS11105 - 2320667..2321323 (-) 657 WP_016659479.1 nuclease-related domain-containing protein -
  ACLKM5_RS11110 - 2321553..2322038 (-) 486 WP_005179878.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  ACLKM5_RS11115 sugE 2322072..2322389 (-) 318 WP_005179881.1 quaternary ammonium compound efflux SMR transporter SugE -
  ACLKM5_RS11120 - 2322701..2323405 (-) 705 WP_411687787.1 copper resistance protein B -
  ACLKM5_RS11125 - 2323680..2325494 (-) 1815 WP_411687784.1 copper resistance system multicopper oxidase -
  ACLKM5_RS11130 - 2325536..2326003 (-) 468 WP_180056333.1 hypothetical protein -
  ACLKM5_RS11135 rpe 2326411..2327097 (-) 687 WP_075175405.1 ribulose-phosphate 3-epimerase -
  ACLKM5_RS11140 - 2327276..2327560 (+) 285 WP_104472524.1 DUF2218 domain-containing protein -
  ACLKM5_RS11145 - 2327577..2328614 (-) 1038 WP_411687783.1 M48 family metallopeptidase -
  ACLKM5_RS11150 - 2328626..2329729 (-) 1104 WP_104489531.1 YjgN family protein -
  ACLKM5_RS11155 fghA 2329849..2330685 (-) 837 WP_104499297.1 S-formylglutathione hydrolase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38237.74 Da        Isoelectric Point: 6.5051

>NTDB_id=1106938 ACLKM5_RS10895 WP_005179768.1 2277407..2278444(+) (pilT) [Acinetobacter indicus strain D10_NDM_tet(X3)]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEDNLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFRDICDYPRGLVLVTGPTGSGKSTTLAAMIDYINDNRYDHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMSTLDQSL
KALVSRGVISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1106938 ACLKM5_RS10895 WP_005179768.1 2277407..2278444(+) (pilT) [Acinetobacter indicus strain D10_NDM_tet(X3)]
ATGGATATTACAGAACTATTGGCCTTTTCGGCAAAAAATGGGGCATCGGATTTACACCTGTCGGCAGGCATGCCGCCACT
GATTCGTGTGGATGGTGAAGTACGCCGCATCAATTTGCCCGCCTTGGAACATAAAGAAGTCCACAAGCTGGTCTATGACA
TCATGAACGACAAACAGCGCCGTGATTATGAAGATAACCTGGAAACCGACTTCTCGTTTGAAGTCCCGGGCGTGGCGCGT
TTCCGTGTTAACGCATTTAACCAGAACCGCGGTGCCGGCGCGGTGTTTCGTACCATTCCCTCTAAAGTGCTGACCATTGA
AGATTTGGGAATGGGTCAAATCTTTAGAGATATCTGTGACTATCCACGTGGTCTAGTGCTGGTCACGGGGCCGACGGGTT
CGGGTAAATCCACCACCCTGGCGGCGATGATTGACTATATCAATGACAATCGCTATGACCACATCCTGACCGTGGAAGAC
CCGATTGAATTTGTGCATCAGTCTAAAAAATGTCTGGTCAACCAGCGTGAAGTACATCGTGATACCCACGGCTTTAATGA
AGCACTACGTTCGGCGCTGCGTGAAGACCCGGATATTATTTTGGTCGGTGAGATGCGTGACCTGGAAACCATCCGTTTGG
CACTTACCGCGGCAGAAACCGGTCACCTGGTCTTTGGTACCCTGCATACCACCTCGGCGGCAAAAACCATTGACCGTGTG
ATTGACGTCTTTCCAGCCGAAGAAAAAGATATGGTGCGTGCCATGCTGTCTGAATCTTTACAGGCGGTCATTTCACAAAC
CCTGCTCAAGAAAAATGGCGGTGGCCGTGTGGCGGCGCATGAAATCATGATCGGGATTCCGGCCATCCGTAACCTGATTC
GTGAAAACAAGGTTGCACAGATGTACTCGGCCATTCAAACCGGTGCCAACTACGGTATGAGTACGCTGGACCAGAGCCTG
AAAGCCCTGGTCAGCCGTGGCGTGATCAGTCCACAGACCGCGCGTACTGTGGCGAAACAGCCAGAATCGTTCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9MH18

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.783

100

0.948
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

97.391

0.672
  pilT Vibrio cholerae strain A1552

69.048

97.391

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

42.09

97.101

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

37.861

100

0.38

Multiple sequence alignment