Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN6MT_RS12030 Genome accession   NZ_CP183883
Coordinates   2508588..2509637 (+) Length   349 a.a.
NCBI ID   WP_419956028.1    Uniprot ID   -
Organism   Neobacillus niacini strain BE6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2503588..2514637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN6MT_RS12005 (ACN6MT_12005) - 2503633..2504109 (-) 477 WP_419956023.1 PulJ/GspJ family protein -
  ACN6MT_RS12010 (ACN6MT_12010) - 2504126..2504596 (-) 471 WP_419956024.1 type IV pilus modification PilV family protein -
  ACN6MT_RS12015 (ACN6MT_12015) - 2504794..2505570 (+) 777 WP_419956025.1 hypothetical protein -
  ACN6MT_RS12020 (ACN6MT_12020) - 2505598..2506896 (+) 1299 WP_419956026.1 G5 domain-containing protein -
  ACN6MT_RS12025 (ACN6MT_12025) - 2506915..2508576 (+) 1662 WP_419956027.1 GspE/PulE family protein -
  ACN6MT_RS12030 (ACN6MT_12030) pilT 2508588..2509637 (+) 1050 WP_419956028.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN6MT_RS12035 (ACN6MT_12035) - 2509630..2510832 (+) 1203 WP_419956029.1 type II secretion system F family protein -
  ACN6MT_RS12040 (ACN6MT_12040) - 2510981..2511457 (+) 477 WP_419956030.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACN6MT_RS12045 (ACN6MT_12045) - 2511474..2511659 (+) 186 Protein_2273 prepilin peptidase -
  ACN6MT_RS12050 (ACN6MT_12050) - 2511753..2512214 (+) 462 WP_419956427.1 prepilin peptidase -
  ACN6MT_RS12055 (ACN6MT_12055) pilM 2512231..2513223 (+) 993 WP_419956031.1 type IV pilus biogenesis protein PilM -
  ACN6MT_RS12060 (ACN6MT_12060) - 2513223..2513918 (+) 696 WP_419956032.1 PilN domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38881.89 Da        Isoelectric Point: 7.6358

>NTDB_id=1106709 ACN6MT_RS12030 WP_419956028.1 2508588..2509637(+) (pilT) [Neobacillus niacini strain BE6]
MKEKLENILRAALEFKASDIHFTVGVPPIFRINGDIKRYGKDILLPDDTEEIAKLTVPENMYDLFKEKGELDYSYGLPGV
SRFRVNAYHQRKSVSLALRVVASKTPTLEDLDLPKIVSELVEKPQGLILVTGPTGSGKSTTLASMIDYVNRTMRKHIVTL
EDPIEYLHRHGNSIIDQREVGFDTQSYANGLKGALRQDPDIILVGEMRDLETIGIAITAAETGHLVLGTLHTSSAPATIN
RIIDVFPPAQQPQIRVQLASVLVGVISQRLFPTVDKKGRKAATEILVNNPAIANLIRNEKIHQIQSTMQTSRAQGMHTLE
MSIRDLIDRNLIQKEAATKYLQEKMMLDG

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=1106709 ACN6MT_RS12030 WP_419956028.1 2508588..2509637(+) (pilT) [Neobacillus niacini strain BE6]
ATGAAAGAAAAACTTGAGAATATACTGCGTGCAGCACTCGAATTTAAAGCGTCCGATATTCATTTTACGGTTGGAGTTCC
TCCGATTTTTCGGATAAATGGCGATATTAAGCGCTATGGAAAAGATATACTTCTCCCAGATGATACCGAAGAAATCGCAA
AACTAACAGTTCCAGAAAATATGTATGATTTATTCAAGGAAAAAGGTGAACTTGATTACTCTTATGGCCTCCCTGGAGTA
TCGCGTTTCCGTGTCAATGCTTACCACCAAAGGAAGAGTGTTTCCCTTGCATTAAGGGTTGTTGCATCAAAAACACCAAC
GCTTGAGGATTTAGATTTACCTAAAATTGTTTCAGAGCTTGTTGAAAAACCACAAGGGTTAATTCTCGTCACAGGTCCGA
CAGGTAGTGGTAAATCAACCACATTGGCCTCAATGATTGATTATGTGAATAGAACGATGCGAAAGCATATTGTTACACTT
GAAGATCCAATTGAGTACTTGCATAGACATGGAAACTCCATTATTGATCAGCGAGAAGTTGGTTTTGATACGCAATCGTA
TGCCAATGGCTTAAAAGGAGCACTACGACAAGATCCAGATATTATTCTTGTTGGGGAAATGCGGGATTTAGAAACAATCG
GAATCGCGATTACAGCAGCAGAAACCGGTCACTTGGTATTGGGAACACTCCATACATCGAGCGCGCCGGCTACGATTAAT
AGGATTATTGACGTATTTCCGCCTGCACAGCAGCCACAAATACGTGTTCAGTTGGCATCGGTTCTAGTAGGAGTAATCTC
ACAGCGTCTTTTTCCAACCGTTGATAAAAAAGGCAGGAAGGCAGCCACTGAAATCTTAGTTAACAATCCTGCCATTGCGA
ACTTAATCAGAAATGAGAAGATTCATCAAATCCAAAGCACGATGCAAACTTCACGTGCTCAAGGAATGCACACTCTTGAA
ATGAGCATCAGAGATTTAATTGACCGCAACTTAATTCAAAAAGAAGCTGCAACCAAATATCTACAAGAAAAGATGATGCT
GGATGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.829

93.983

0.487
  pilT Neisseria gonorrhoeae MS11

47.522

98.281

0.467
  pilT Neisseria meningitidis 8013

47.23

98.281

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.507

97.708

0.464
  pilT Vibrio cholerae strain A1552

47.507

97.708

0.464
  pilT Acinetobacter baylyi ADP1

46.866

95.989

0.45
  pilT Pseudomonas stutzeri DSM 10701

47.576

94.556

0.45
  pilT Pseudomonas aeruginosa PAK

47.112

94.269

0.444
  pilT Legionella pneumophila strain Lp02

45.562

96.848

0.441
  pilT Legionella pneumophila strain ERS1305867

45.562

96.848

0.441
  pilT Acinetobacter baumannii D1279779

46.364

94.556

0.438
  pilT Acinetobacter nosocomialis M2

46.364

94.556

0.438
  pilT Acinetobacter baumannii strain A118

46.364

94.556

0.438
  pilU Pseudomonas stutzeri DSM 10701

41.018

95.702

0.393
  pilU Acinetobacter baylyi ADP1

40.059

96.562

0.387
  pilU Vibrio cholerae strain A1552

39.222

95.702

0.375

Multiple sequence alignment