Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN6MY_RS20480 Genome accession   NZ_CP183879
Coordinates   4092044..4093084 (-) Length   346 a.a.
NCBI ID   WP_090989615.1    Uniprot ID   -
Organism   Peribacillus sp. B-H-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4087044..4098084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN6MY_RS20455 (ACN6MY_20455) - 4087810..4088484 (-) 675 WP_419881810.1 hypothetical protein -
  ACN6MY_RS20460 (ACN6MY_20460) pilM 4088481..4089479 (-) 999 WP_419881811.1 type IV pilus biogenesis protein PilM -
  ACN6MY_RS20465 (ACN6MY_20465) - 4089504..4090262 (-) 759 WP_419881812.1 prepilin peptidase -
  ACN6MY_RS20470 (ACN6MY_20470) - 4090296..4090727 (-) 432 WP_419881813.1 type II secretion system protein -
  ACN6MY_RS20475 (ACN6MY_20475) - 4090835..4092043 (-) 1209 WP_419881814.1 type II secretion system F family protein -
  ACN6MY_RS20480 (ACN6MY_20480) pilT 4092044..4093084 (-) 1041 WP_090989615.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN6MY_RS20485 (ACN6MY_20485) - 4093094..4094755 (-) 1662 WP_419881815.1 GspE/PulE family protein -
  ACN6MY_RS20490 (ACN6MY_20490) - 4094752..4096257 (-) 1506 WP_419881816.1 G5 domain-containing protein -
  ACN6MY_RS20495 (ACN6MY_20495) - 4096277..4096996 (-) 720 WP_419881817.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38697.71 Da        Isoelectric Point: 8.0121

>NTDB_id=1106649 ACN6MY_RS20480 WP_090989615.1 4092044..4093084(-) (pilT) [Peribacillus sp. B-H-3]
MNTKIDHLLRAGFELKASDIHITVGVPPIMRLNGELKKYGKEELRPEDTEGMAKSIIPENMWKQLKEKGELDFSYGIPGV
SRFRINTYFQRSCIAMAIRIVPTRIPTIEELKLPDTLKKITEKPQGLVLVTGPTGSGKSTTLASMIQYMNHTMRKHIITL
EDPIEYLHKHQNCIIDQREVGFDTNNFANGLRAALRQDPDVILVGEMRDLETIHTAITAAETGHLVFGTLHTSSAPSTID
RIIDVFEPAQQAQVRIQLASVLVSIVSQRLFPTVDKKGRRAATEILVNNSAVANLIRNEKIHQIVNIMQTSRAQGMHTLD
SDIKELLTAGVISKESAFSFLQDKVE

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1106649 ACN6MY_RS20480 WP_090989615.1 4092044..4093084(-) (pilT) [Peribacillus sp. B-H-3]
ATGAATACAAAAATTGATCACTTGCTTAGAGCCGGCTTTGAATTGAAGGCTTCTGATATCCATATAACCGTCGGTGTGCC
GCCCATCATGCGCTTGAATGGCGAGCTGAAGAAGTACGGCAAAGAAGAGCTGCGCCCAGAGGATACGGAAGGAATGGCCA
AGTCCATCATTCCCGAGAATATGTGGAAACAGTTAAAGGAAAAAGGGGAGCTAGATTTTTCCTATGGAATTCCCGGTGTC
TCCAGGTTCAGGATTAACACCTATTTTCAGCGCTCCTGCATTGCCATGGCTATCAGGATTGTCCCGACAAGGATTCCGAC
CATTGAAGAGCTGAAGCTTCCGGATACGTTAAAAAAGATTACCGAGAAGCCACAAGGGCTTGTGCTTGTGACTGGGCCTA
CTGGAAGCGGGAAGTCAACAACGCTTGCCTCCATGATTCAATATATGAATCATACAATGAGGAAGCATATTATTACCCTT
GAAGATCCTATTGAATATTTGCACAAGCATCAAAACTGCATCATTGACCAGCGTGAGGTCGGCTTTGATACGAATAACTT
CGCCAATGGGCTGCGGGCGGCACTGAGACAGGATCCGGATGTTATTTTAGTCGGGGAAATGAGGGATCTTGAAACGATCC
ATACTGCCATTACGGCAGCAGAAACGGGTCACCTTGTTTTCGGCACACTCCATACCTCAAGCGCTCCATCAACGATTGAC
AGAATCATCGATGTCTTCGAGCCTGCACAGCAGGCGCAGGTCAGGATTCAGCTTGCCTCAGTGCTCGTGTCGATTGTATC
GCAGAGGCTGTTCCCGACAGTGGATAAAAAGGGACGGAGAGCGGCAACGGAAATACTTGTCAACAACTCAGCGGTAGCCA
ACCTGATCCGAAACGAAAAAATCCATCAGATTGTGAATATCATGCAAACATCACGGGCCCAGGGCATGCATACATTGGAT
AGTGATATCAAAGAGCTTTTGACAGCAGGAGTTATTTCAAAGGAAAGTGCATTTTCATTTTTGCAGGATAAGGTGGAGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.368

95.087

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.397

99.133

0.48
  pilT Vibrio cholerae strain A1552

48.397

99.133

0.48
  pilT Neisseria meningitidis 8013

48.204

96.532

0.465
  pilT Neisseria gonorrhoeae MS11

47.904

96.532

0.462
  pilT Legionella pneumophila strain ERS1305867

47.447

96.243

0.457
  pilT Legionella pneumophila strain Lp02

47.447

96.243

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.147

96.243

0.454
  pilT Acinetobacter baumannii D1279779

46.847

96.243

0.451
  pilT Pseudomonas aeruginosa PAK

46.847

96.243

0.451
  pilT Acinetobacter nosocomialis M2

46.847

96.243

0.451
  pilT Acinetobacter baumannii strain A118

46.847

96.243

0.451
  pilT Acinetobacter baylyi ADP1

46.246

96.243

0.445
  pilU Pseudomonas stutzeri DSM 10701

44.776

96.821

0.434
  pilU Acinetobacter baylyi ADP1

41.049

93.642

0.384
  pilU Vibrio cholerae strain A1552

40.557

93.353

0.379

Multiple sequence alignment