Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN6MX_RS11970 Genome accession   NZ_CP183878
Coordinates   2441920..2442960 (-) Length   346 a.a.
NCBI ID   WP_419959137.1    Uniprot ID   -
Organism   Psychrobacillus sp. BM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2436920..2447960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN6MX_RS11945 (ACN6MX_11945) - 2437790..2438479 (-) 690 WP_419959132.1 potassium transporter -
  ACN6MX_RS11950 (ACN6MX_11950) - 2438479..2439075 (-) 597 WP_419959133.1 hypothetical protein -
  ACN6MX_RS11955 (ACN6MX_11955) pilM 2439075..2440049 (-) 975 WP_419959134.1 type IV pilus biogenesis protein PilM -
  ACN6MX_RS11960 (ACN6MX_11960) - 2440185..2440622 (-) 438 WP_419959135.1 type II secretion system protein -
  ACN6MX_RS11965 (ACN6MX_11965) - 2440715..2441920 (-) 1206 WP_419959136.1 type II secretion system F family protein -
  ACN6MX_RS11970 (ACN6MX_11970) pilT 2441920..2442960 (-) 1041 WP_419959137.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN6MX_RS11975 (ACN6MX_11975) - 2442972..2444636 (-) 1665 WP_419959138.1 GspE/PulE family protein -
  ACN6MX_RS11980 (ACN6MX_11980) - 2444659..2446017 (-) 1359 WP_419959139.1 VanW family protein -
  ACN6MX_RS11985 (ACN6MX_11985) - 2446357..2446788 (+) 432 WP_419959140.1 type IV pilus modification PilV family protein -
  ACN6MX_RS11990 (ACN6MX_11990) - 2446767..2447216 (+) 450 WP_419959141.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38303.85 Da        Isoelectric Point: 6.6394

>NTDB_id=1106581 ACN6MX_RS11970 WP_419959137.1 2441920..2442960(-) (pilT) [Psychrobacillus sp. BM2]
MKISIIDLLEKAFHDRASDLHITVGIPPVFRIHGALQSFGESILRESDTKDILLEIIPDSQQQVFDEKGEIDFSYELPGR
CRFRINAYHQQGNVSIAARMIDSQIPAIESLGMPNVLYTLADKQQGLILVTGPTGSGKSTTLASMIDYINSTKSKHIITL
EDPIEYVHTHKKSIINQREVGEDTRSFANGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVMATLHTSSAPTTID
RIIDVFPPHQQGQIRIQLANVLQGIISQRLFVRKDAKGRIAATEILLQTPSVANLIRNEKVHQIQNVMQTSRALGMHTLE
SSIQQLVTSGKISLESAKPYLNVGEY

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1106581 ACN6MX_RS11970 WP_419959137.1 2441920..2442960(-) (pilT) [Psychrobacillus sp. BM2]
ATGAAGATTTCAATCATTGACTTATTAGAAAAAGCGTTTCATGATAGAGCCTCCGATTTGCATATTACCGTTGGAATTCC
TCCGGTATTTAGAATACACGGAGCCCTTCAATCATTTGGAGAATCAATTTTAAGAGAATCAGATACAAAAGATATTCTTC
TTGAAATAATTCCTGATTCTCAACAACAAGTGTTTGATGAAAAAGGAGAGATTGATTTTAGTTATGAATTACCTGGTCGT
TGCCGATTCCGTATCAATGCTTATCATCAACAGGGAAATGTGTCGATTGCAGCACGTATGATTGATTCGCAAATACCTGC
TATTGAATCACTTGGTATGCCAAATGTACTTTATACATTAGCAGACAAGCAACAGGGACTGATTCTAGTTACTGGTCCCA
CTGGTTCTGGGAAATCTACCACACTTGCATCGATGATAGACTATATCAACTCTACAAAATCCAAGCATATTATTACGTTA
GAGGATCCGATTGAGTATGTGCATACGCATAAGAAATCTATCATTAACCAGCGTGAAGTTGGGGAAGATACCAGGAGCTT
TGCGAATGGCTTAAGGGCTGCGCTTCGTCAGGACCCAGATGTCATTTTAGTCGGAGAAATGCGTGATTTAGAAACCATTT
CTACTGCTATAACTGCTGCAGAAACGGGTCATTTAGTGATGGCAACACTGCATACCAGTAGTGCACCTACTACAATTGAC
AGGATAATAGATGTCTTCCCTCCACATCAGCAAGGGCAAATACGTATTCAGCTCGCAAATGTTCTGCAAGGAATTATTTC
CCAAAGACTATTTGTTCGTAAAGATGCAAAGGGGCGTATTGCGGCCACAGAGATTTTGTTGCAAACACCGTCCGTTGCAA
ACTTGATACGTAATGAAAAAGTACATCAAATACAAAACGTTATGCAGACAAGTCGTGCATTAGGTATGCATACGCTCGAA
TCTTCCATTCAACAATTAGTTACATCAGGAAAAATATCACTCGAATCTGCTAAACCCTACTTGAATGTAGGTGAGTATTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.012

93.642

0.506
  pilT Vibrio cholerae strain A1552

50.909

95.376

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.909

95.376

0.486
  pilT Pseudomonas aeruginosa PAK

48.688

99.133

0.483
  pilT Pseudomonas stutzeri DSM 10701

49.405

97.11

0.48
  pilT Acinetobacter baylyi ADP1

49.107

97.11

0.477
  pilT Acinetobacter nosocomialis M2

48.214

97.11

0.468
  pilT Neisseria meningitidis 8013

48.214

97.11

0.468
  pilT Acinetobacter baumannii D1279779

48.214

97.11

0.468
  pilT Acinetobacter baumannii strain A118

48.214

97.11

0.468
  pilT Neisseria gonorrhoeae MS11

47.917

97.11

0.465
  pilT Legionella pneumophila strain Lp02

48.06

96.821

0.465
  pilT Legionella pneumophila strain ERS1305867

48.06

96.821

0.465
  pilU Vibrio cholerae strain A1552

43.168

93.064

0.402
  pilU Pseudomonas stutzeri DSM 10701

42.056

92.775

0.39
  pilU Acinetobacter baylyi ADP1

39.759

95.954

0.382

Multiple sequence alignment