Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN2W4_RS19050 Genome accession   NZ_CP183425
Coordinates   3976389..3977411 (+) Length   340 a.a.
NCBI ID   WP_419685961.1    Uniprot ID   -
Organism   Serratia marcescens strain H-12-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3971389..3982411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN2W4_RS19010 (ACN2W4_19010) - 3971691..3971933 (+) 243 WP_004937418.1 DUF4160 domain-containing protein -
  ACN2W4_RS19015 (ACN2W4_19015) - 3971917..3972165 (+) 249 WP_038879292.1 DUF2442 domain-containing protein -
  ACN2W4_RS19020 (ACN2W4_19020) hemW 3972201..3973343 (-) 1143 WP_342137470.1 radical SAM family heme chaperone HemW -
  ACN2W4_RS19025 (ACN2W4_19025) - 3973336..3973929 (-) 594 WP_038879287.1 XTP/dITP diphosphatase -
  ACN2W4_RS19030 (ACN2W4_19030) yggU 3973947..3974237 (-) 291 WP_033649585.1 DUF167 family protein YggU -
  ACN2W4_RS19035 (ACN2W4_19035) - 3974234..3974788 (-) 555 WP_033635944.1 YggT family protein -
  ACN2W4_RS19040 (ACN2W4_19040) proC 3974820..3975641 (-) 822 WP_342138550.1 pyrroline-5-carboxylate reductase -
  ACN2W4_RS19045 (ACN2W4_19045) - 3975661..3976368 (-) 708 WP_192974193.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN2W4_RS19050 (ACN2W4_19050) pilT 3976389..3977411 (+) 1023 WP_419685961.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN2W4_RS19055 (ACN2W4_19055) ruvX 3977408..3977830 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  ACN2W4_RS19060 (ACN2W4_19060) - 3977830..3978393 (-) 564 WP_033635940.1 YqgE/AlgH family protein -
  ACN2W4_RS19065 (ACN2W4_19065) gshB 3978539..3979489 (-) 951 WP_038879277.1 glutathione synthase -
  ACN2W4_RS19070 (ACN2W4_19070) rsmE 3979500..3980231 (-) 732 WP_308322102.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACN2W4_RS19075 (ACN2W4_19075) endA 3980301..3980996 (-) 696 WP_038879275.1 deoxyribonuclease I -
  ACN2W4_RS19080 (ACN2W4_19080) - 3981098..3981610 (-) 513 WP_225527964.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36444.78 Da        Isoelectric Point: 6.8839

>NTDB_id=1105715 ACN2W4_RS19050 WP_419685961.1 3976389..3977411(+) (pilT) [Serratia marcescens strain H-12-3]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQVPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGD

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1105715 ACN2W4_RS19050 WP_419685961.1 3976389..3977411(+) (pilT) [Serratia marcescens strain H-12-3]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGTCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGCGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGTGCCTTCGCT
TGCCGAGCTGGCGGCCCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTAACGCTGGAA
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATTCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATATCGCCACCATTCGGT
TGGCGCTCACTGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGCTGCACACCCGTAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGTGCCCAGTTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGCGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGTAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(68-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.662

97.353

0.503
  pilT Neisseria meningitidis 8013

50.298

98.824

0.497
  pilT Acinetobacter baumannii strain A118

51.212

97.059

0.497
  pilT Acinetobacter nosocomialis M2

51.212

97.059

0.497
  pilT Acinetobacter baumannii D1279779

51.212

97.059

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

97.647

0.491
  pilT Pseudomonas aeruginosa PAK

50

97.059

0.485
  pilT Legionella pneumophila strain ERS1305867

49.547

97.353

0.482
  pilT Legionella pneumophila strain Lp02

49.547

97.353

0.482
  pilT Pseudomonas stutzeri DSM 10701

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.456
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371

Multiple sequence alignment