Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACNVED_RS11580 Genome accession   NZ_CP183303
Coordinates   2689714..2690751 (-) Length   345 a.a.
NCBI ID   WP_419419169.1    Uniprot ID   -
Organism   Legionella sp. D16C41     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2684714..2695751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNVED_RS11555 (ACNVED_11555) gmk 2684810..2685439 (-) 630 WP_419419164.1 guanylate kinase -
  ACNVED_RS11560 (ACNVED_11560) - 2685443..2686309 (-) 867 WP_419419165.1 YicC/YloC family endoribonuclease -
  ACNVED_RS11565 (ACNVED_11565) rph 2686538..2687245 (+) 708 WP_419419166.1 ribonuclease PH -
  ACNVED_RS11570 (ACNVED_11570) - 2687309..2688088 (+) 780 WP_419419167.1 hypothetical protein -
  ACNVED_RS11575 (ACNVED_11575) - 2688214..2689686 (+) 1473 WP_419419168.1 hypothetical protein -
  ACNVED_RS11580 (ACNVED_11580) pilT 2689714..2690751 (-) 1038 WP_419419169.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNVED_RS11585 (ACNVED_11585) - 2691039..2691728 (+) 690 WP_419419170.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNVED_RS11590 (ACNVED_11590) proC 2691732..2692529 (+) 798 WP_419419171.1 pyrroline-5-carboxylate reductase -
  ACNVED_RS11595 (ACNVED_11595) - 2692714..2693289 (+) 576 WP_419419172.1 YggT family protein -
  ACNVED_RS11600 (ACNVED_11600) - 2693536..2694132 (+) 597 WP_419419173.1 DUF2845 domain-containing protein -
  ACNVED_RS11605 (ACNVED_11605) - 2694152..2694454 (+) 303 WP_419419174.1 hypothetical protein -
  ACNVED_RS11610 (ACNVED_11610) - 2694526..2695245 (-) 720 WP_419419175.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38411.14 Da        Isoelectric Point: 6.4313

>NTDB_id=1105187 ACNVED_RS11580 WP_419419169.1 2689714..2690751(-) (pilT) [Legionella sp. D16C41]
MNITELLAFSVKNKSSDLHLSAGLPPLIRVDGDLRRINIPPLEHREVIDVVYDIMNDKQRKDFEQHLETDFSFEIPNLAR
FRVNIFNQVRGAGAVFRTIPSHILGIEELGLPPIFQTIASYPHGLVLITGPTGSGKSTTLAALIDYINSTRYEHILTIED
PVEFLHESKKCLLNQREVHRDTESFNAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTNSATKTINRI
IDVFPGDEKAMVRTMLSESLQAVVSQVLLKKYGGGRVAALEIMICNAAIRNLIREDKVAQMYSTIQTGQSQGMQTLDQHL
TQLVQNKIIAKSAAQEIAVNKAVFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1105187 ACNVED_RS11580 WP_419419169.1 2689714..2690751(-) (pilT) [Legionella sp. D16C41]
ATGAATATTACTGAATTGTTGGCTTTTTCAGTGAAAAACAAATCATCAGATCTACATTTATCGGCAGGCCTTCCTCCCTT
AATTCGTGTCGATGGTGACTTACGACGGATTAATATTCCACCCTTAGAGCATAGAGAAGTTATTGACGTCGTATATGATA
TTATGAATGATAAACAGCGTAAGGATTTTGAACAGCACTTAGAAACAGACTTTTCATTTGAGATACCGAATCTTGCTCGC
TTTCGTGTCAATATATTTAATCAAGTTCGCGGTGCTGGTGCTGTGTTTAGAACTATTCCCAGCCATATATTGGGTATTGA
GGAGTTAGGTTTACCTCCTATTTTCCAAACGATTGCTTCTTACCCCCATGGGTTAGTATTAATTACAGGCCCCACAGGCT
CCGGAAAAAGTACAACCCTTGCGGCGTTAATTGATTATATTAATTCCACTCGCTATGAACATATCTTAACTATTGAAGAT
CCAGTCGAGTTTTTACATGAAAGTAAAAAATGTTTGCTTAATCAAAGAGAAGTACATCGAGATACGGAAAGTTTTAACGC
AGCACTTCGCTCGGCCCTACGCGAAGATCCAGATGTAATTTTAGTTGGCGAATTACGTGATTTAGAAACCATTCGTCTTG
CCCTAACTGCCGCAGAAACAGGCCACTTAGTATTTGGTACCTTACATACAAATTCTGCTACTAAAACAATTAATCGTATT
ATTGATGTCTTTCCAGGCGACGAGAAAGCCATGGTGCGTACTATGCTTTCTGAGTCCCTACAAGCTGTCGTTTCACAAGT
TTTACTTAAAAAATATGGTGGTGGGCGAGTTGCTGCATTAGAAATTATGATTTGTAATGCTGCCATTCGCAATTTAATTC
GCGAAGATAAGGTTGCGCAAATGTATTCAACCATTCAAACAGGTCAATCACAAGGCATGCAGACGCTTGACCAACATTTA
ACACAATTAGTGCAGAATAAAATCATTGCCAAGAGTGCCGCGCAAGAGATAGCTGTTAATAAGGCAGTGTTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

79.36

99.71

0.791
  pilT Legionella pneumophila strain ERS1305867

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

74.419

99.71

0.742
  pilT Acinetobacter baumannii D1279779

73.547

99.71

0.733
  pilT Acinetobacter nosocomialis M2

73.547

99.71

0.733
  pilT Acinetobacter baumannii strain A118

73.547

99.71

0.733
  pilT Pseudomonas aeruginosa PAK

74.556

97.971

0.73
  pilT Pseudomonas stutzeri DSM 10701

73.373

97.971

0.719
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.982

98.841

0.652
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.579

97.681

0.641
  pilT Vibrio cholerae strain A1552

65.579

97.681

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

38.393

97.391

0.374

Multiple sequence alignment