Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACN08S_RS18695 Genome accession   NZ_CP183265
Coordinates   1509277..1510314 (-) Length   345 a.a.
NCBI ID   WP_008987964.1    Uniprot ID   A0A0U1P8W8
Organism   Photobacterium leiognathi subsp. mandapamensis strain Sr2.36     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1504277..1515314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08S_RS18670 (ACN08S_18705) rsmE 1504977..1505708 (+) 732 WP_419208925.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACN08S_RS18675 (ACN08S_18710) gshB 1505727..1506677 (+) 951 WP_419208926.1 glutathione synthase -
  ACN08S_RS18680 (ACN08S_18715) - 1506801..1507364 (+) 564 WP_008987967.1 YqgE/AlgH family protein -
  ACN08S_RS18685 (ACN08S_18720) ruvX 1507361..1507789 (+) 429 WP_023933568.1 Holliday junction resolvase RuvX -
  ACN08S_RS18690 (ACN08S_18725) pilU 1508139..1509242 (-) 1104 WP_008987965.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN08S_RS18695 (ACN08S_18730) pilT 1509277..1510314 (-) 1038 WP_008987964.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN08S_RS18700 (ACN08S_18735) - 1510337..1511047 (+) 711 WP_419208927.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN08S_RS18705 (ACN08S_18740) proC 1511134..1511952 (+) 819 WP_419208928.1 pyrroline-5-carboxylate reductase -
  ACN08S_RS18710 (ACN08S_18745) - 1511962..1512516 (+) 555 WP_008987961.1 YggT family protein -
  ACN08S_RS18715 (ACN08S_18750) yggU 1512513..1512806 (+) 294 WP_045069242.1 DUF167 family protein YggU -
  ACN08S_RS18720 (ACN08S_18755) - 1512952..1513377 (+) 426 WP_008987959.1 DUF4426 domain-containing protein -
  ACN08S_RS18725 (ACN08S_18760) - 1513497..1514090 (+) 594 WP_045064316.1 XTP/dITP diphosphatase -
  ACN08S_RS18730 (ACN08S_18765) hemW 1514091..1515254 (+) 1164 WP_419208929.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38372.91 Da        Isoelectric Point: 6.0865

>NTDB_id=1104923 ACN08S_RS18695 WP_008987964.1 1509277..1510314(-) (pilT) [Photobacterium leiognathi subsp. mandapamensis strain Sr2.36]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSMPALDHSEVHRLVFDIMNDAQRREYEENLEVDFSFEIPDIGR
FRVNAFHQSRGCSAVLRTIPIHIPTLESLNVPDVFYDIAQLKRGLVLVTGATGSGKSTTIAALVDYINSNYNRHILTIED
PIEFVHQSKRCLINQREVHRDTLGFQNALRSALREDPDVILVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTVDRI
IDVFPGADKSMVRSMLSESLRAVISQSLLKRVEGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSAMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSSQRGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1104923 ACN08S_RS18695 WP_008987964.1 1509277..1510314(-) (pilT) [Photobacterium leiognathi subsp. mandapamensis strain Sr2.36]
ATGGATATCACCGAACTACTGGATTTTAGTGTAAAGCATAACGCATCGGATCTACATCTTTCTGCAGGTGTTCCTCCCAT
GATCCGTGTCGATGGCGATGTGAGAAAATTAAGCATGCCAGCGTTAGATCATAGTGAAGTGCATCGCTTAGTGTTTGACA
TTATGAACGATGCCCAACGTCGTGAGTATGAAGAAAACTTAGAAGTCGATTTTTCATTTGAAATTCCCGACATTGGTCGC
TTCAGGGTTAATGCTTTCCACCAATCTCGTGGTTGCTCTGCTGTATTACGTACGATCCCAATCCATATTCCGACATTGGA
ATCGTTAAATGTGCCTGACGTGTTCTACGATATTGCTCAGCTTAAGCGTGGTTTAGTGCTGGTAACAGGTGCAACAGGTT
CAGGTAAATCGACCACAATTGCTGCCTTGGTTGATTATATCAATAGCAACTATAACCGTCATATTTTGACGATCGAAGAT
CCGATCGAATTTGTGCACCAAAGCAAGCGTTGTTTGATCAACCAGCGTGAAGTGCACCGTGATACGTTAGGCTTCCAGAA
CGCACTGCGTTCAGCACTGCGTGAAGATCCCGATGTCATTTTAGTGGGTGAGCTTCGTGATCAGGAAACAATCAGTTTAG
CGTTAACAGCAGCTGAAACAGGACACTTAGTTTTAGGTACTTTGCATACAAGTTCTGCGGCAAAAACGGTCGACCGTATT
ATTGACGTATTCCCAGGTGCAGATAAATCGATGGTGCGTTCAATGTTGTCTGAATCACTGCGTGCGGTAATTTCTCAAAG
TCTATTAAAGCGTGTCGAAGGTGGTCGTGTTGCATCTCATGAAATCATGATGGCAACGCCAGCGATCCGAAACTTGATCC
GTGAAGATAAGATCGCGCAAATGTACTCGATGATCCAAACCGGTTCAGCAATGGGCATGCAGACCATGGAGCAAAGCGTC
AAGATGCTGGTGGCGCAAGGTTTAGTGGAAGCTGAAGAAGGGCGTCGTATTGTTGATAGTAGTCAGCGAGGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U1P8W8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.812

100

0.768
  pilT Vibrio cholerae strain A1552

76.812

100

0.768
  pilT Acinetobacter baumannii strain A118

71.045

97.101

0.69
  pilT Acinetobacter baumannii D1279779

71.045

97.101

0.69
  pilT Acinetobacter baylyi ADP1

69.298

99.13

0.687
  pilT Acinetobacter nosocomialis M2

70.746

97.101

0.687
  pilT Pseudomonas stutzeri DSM 10701

69.851

97.101

0.678
  pilT Pseudomonas aeruginosa PAK

69.254

97.101

0.672
  pilT Legionella pneumophila strain Lp02

69.605

95.362

0.664
  pilT Legionella pneumophila strain ERS1305867

69.605

95.362

0.664
  pilT Neisseria meningitidis 8013

65.306

99.42

0.649
  pilT Neisseria gonorrhoeae MS11

65.015

99.42

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.419

99.71

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.566

99.42

0.423
  pilU Vibrio cholerae strain A1552

41.52

99.13

0.412
  pilU Acinetobacter baylyi ADP1

39.752

93.333

0.371

Multiple sequence alignment