Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACBO77_RS15040 Genome accession   NZ_CP182134
Coordinates   3463376..3464413 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas arboricola pv. pruni strain Xap 9-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3458376..3469413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACBO77_RS15015 ruvX 3459119..3459586 (-) 468 WP_039513833.1 Holliday junction resolvase RuvX -
  ACBO77_RS15020 - 3459579..3460145 (-) 567 WP_150861924.1 YqgE/AlgH family protein -
  ACBO77_RS15025 - 3460480..3461025 (+) 546 WP_039813256.1 DNA-3-methyladenine glycosylase I -
  ACBO77_RS15030 - 3461163..3461837 (-) 675 WP_024940756.1 YitT family protein -
  ACBO77_RS15035 pilU 3462123..3463253 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACBO77_RS15040 pilT 3463376..3464413 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACBO77_RS15045 - 3464736..3465428 (+) 693 WP_039513823.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACBO77_RS15050 proC 3465490..3466335 (+) 846 WP_039813263.1 pyrroline-5-carboxylate reductase -
  ACBO77_RS15055 - 3466933..3467358 (+) 426 WP_026064288.1 HU family DNA-binding protein -
  ACBO77_RS15060 - 3467494..3468114 (+) 621 WP_039513819.1 M23 family metallopeptidase -
  ACBO77_RS15065 - 3468206..3468514 (+) 309 WP_024940752.1 DUF3861 family protein -
  ACBO77_RS15070 - 3468794..3469114 (+) 321 WP_080639692.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=1100521 ACBO77_RS15040 WP_003489919.1 3463376..3464413(-) (pilT) [Xanthomonas arboricola pv. pruni strain Xap 9-4]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1100521 ACBO77_RS15040 WP_003489919.1 3463376..3464413(-) (pilT) [Xanthomonas arboricola pv. pruni strain Xap 9-4]
ATGGATATCGCTGAACTATTGGCGTTTTCCGTCAAAAACAAGGCATCGGACCTGCATCTGTCGGCCGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCCCTGGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCACGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGCACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTTCGCCAGTTGATCGACCAGCCGCAGGGTTTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACGGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGATCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGACGCACCGCGGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTTGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCACGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment