Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACNFWO_RS01175 Genome accession   NZ_CP181386
Coordinates   259576..260619 (+) Length   347 a.a.
NCBI ID   WP_132648923.1    Uniprot ID   A0A4R2M336
Organism   Rubrivivax gelatinosus isolate YF6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 230604..258851 259576..260619 flank 725
IS/Tn 257879..258829 259576..260619 flank 747


Gene organization within MGE regions


Location: 230604..260619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNFWO_RS01055 tgt 231204..232334 (-) 1131 WP_132648957.1 tRNA guanosine(34) transglycosylase Tgt -
  ACNFWO_RS01060 queA 232350..233384 (-) 1035 WP_132648955.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  ACNFWO_RS01065 recG 233459..235498 (+) 2040 WP_132648954.1 ATP-dependent DNA helicase RecG -
  ACNFWO_RS01070 - 235576..237504 (+) 1929 WP_132648952.1 hypothetical protein -
  ACNFWO_RS01075 proC 237554..238369 (+) 816 WP_132648950.1 pyrroline-5-carboxylate reductase -
  ACNFWO_RS01080 - 238523..239290 (+) 768 WP_132648949.1 hypothetical protein -
  ACNFWO_RS01085 - 239287..240432 (+) 1146 WP_132648947.1 M48 family metalloprotease -
  ACNFWO_RS01090 - 240429..241025 (+) 597 WP_132648946.1 hypothetical protein -
  ACNFWO_RS01095 ubiA 241038..241922 (-) 885 WP_132648945.1 4-hydroxybenzoate octaprenyltransferase -
  ACNFWO_RS01100 - 242132..243394 (+) 1263 WP_132648943.1 putative DNA modification/repair radical SAM protein -
  ACNFWO_RS01105 - 243391..244227 (+) 837 WP_242478489.1 TIGR03915 family putative DNA repair protein -
  ACNFWO_RS01110 - 244238..245878 (-) 1641 WP_043817343.1 peptide chain release factor 3 -
  ACNFWO_RS01115 - 246207..246710 (-) 504 WP_132648942.1 Dps family protein -
  ACNFWO_RS01120 - 247005..248546 (-) 1542 WP_132648940.1 methyl-accepting chemotaxis protein -
  ACNFWO_RS01125 - 248729..249652 (-) 924 WP_132648939.1 LysR substrate-binding domain-containing protein -
  ACNFWO_RS01130 - 249823..251097 (+) 1275 WP_132648937.1 Glu/Leu/Phe/Val family dehydrogenase -
  ACNFWO_RS01135 - 251204..251860 (-) 657 WP_132648936.1 OmpA family protein -
  ACNFWO_RS01140 - 251982..252950 (+) 969 WP_132648934.1 tripartite tricarboxylate transporter substrate binding protein -
  ACNFWO_RS01145 - 252972..253748 (+) 777 WP_132648932.1 tetratricopeptide repeat protein -
  ACNFWO_RS01150 - 253780..256809 (-) 3030 WP_132648930.1 L-glutamate gamma-semialdehyde dehydrogenase -
  ACNFWO_RS01155 - 256903..257403 (+) 501 WP_132648929.1 Lrp/AsnC ligand binding domain-containing protein -
  ACNFWO_RS01160 - 257571..257780 (+) 210 WP_132648927.1 hypothetical protein -
  ACNFWO_RS01165 - 257879..258829 (+) 951 WP_416461459.1 IS481 family transposase -
  ACNFWO_RS01170 - 258841..259533 (-) 693 WP_132648925.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNFWO_RS01175 pilT 259576..260619 (+) 1044 WP_132648923.1 type IV pilus twitching motility protein PilT Machinery gene

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38051.83 Da        Isoelectric Point: 7.3203

>NTDB_id=1099910 ACNFWO_RS01175 WP_132648923.1 259576..260619(+) (pilT) [Rubrivivax gelatinosus isolate YF6]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLDHKMVHAMVYDIMNDAQRKHYEDTLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLGTPKVFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHVLTVED
PIEFVHESKKCLVNQREVGPHTLSFANALRSALREDPDAVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLVAVISQTLCKLKDGSGRVAAHEIMIATSAIKNLIRENKIAQMYSSIQTGQSLGMQTLDQN
LADLVRRNVITPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1099910 ACNFWO_RS01175 WP_132648923.1 259576..260619(+) (pilT) [Rubrivivax gelatinosus isolate YF6]
ATGGACATCACCCAGCTGCTCGCATTCTCGGTCAAGAACAAGGCCTCGGACCTGCACCTGTCGGCTGGGCTGCCGCCGAT
GATCCGCGTGCACGGCGATGTGCGTCGAATCAATGTCGAACCGCTGGACCACAAGATGGTCCACGCGATGGTCTACGACA
TCATGAACGACGCCCAGCGCAAGCACTACGAGGACACGCTGGAGTGCGACTTCAGCTTCGAGATCCAGGGCCTGGCGCGT
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACGATCCCGAGCAAGATCCTGACGCTGGA
GCAGCTGGGCACGCCCAAGGTCTTTGCCGACCTGGCCTTGAAGCCGCGTGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGAGCACGACGCTGGCGGCGATGGTCAACCACCTCAACGAGAACGAGTACGGCCACGTGCTCACCGTCGAGGAC
CCGATCGAGTTCGTGCACGAGAGCAAGAAGTGCCTGGTCAACCAGCGCGAGGTCGGCCCGCACACGCTGTCCTTTGCCAA
CGCGCTGCGTTCGGCGCTGCGCGAGGACCCGGACGCGGTGCTGGTCGGCGAAATGCGCGACCTGGAGACGATCCGCCTGG
CGCTGACCGCCGCCGAGACCGGCCACCTCGTGTTCGGCACGCTGCACACCTCGAGCGCCGCCAAGACCATCGACCGCGTC
GTCGACGTCTTCCCGGCGGCCGAGAAGGAGATGGTGCGCGCGATGCTGTCGGAGTCGCTGGTCGCGGTGATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGCTCGGGGCGCGTGGCCGCGCACGAGATCATGATCGCCACCTCGGCGATCAAGAACCTGA
TCCGCGAGAACAAGATCGCCCAGATGTACTCGTCGATCCAGACCGGCCAGAGCCTGGGCATGCAGACGCTGGACCAGAAC
CTCGCCGACCTGGTGCGCCGCAACGTCATCACGCCGGCCGAGGCGCGGGGCAAGGCGAAGATTCCCGAGAACTTCCCCGG
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R2M336

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

71.386

97.695

0.697
  pilT Legionella pneumophila strain ERS1305867

71.386

97.695

0.697
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.396

98.271

0.643
  pilT Vibrio cholerae strain A1552

65.396

98.271

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403

Multiple sequence alignment