Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACM530_RS11805 Genome accession   NZ_CP181333
Coordinates   2565186..2566220 (+) Length   344 a.a.
NCBI ID   WP_416364824.1    Uniprot ID   -
Organism   Pseudomonas sp. NFX183     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2560186..2571220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM530_RS11775 (ACM530_11775) - 2560541..2560975 (-) 435 WP_106575685.1 DUF4426 domain-containing protein -
  ACM530_RS11780 (ACM530_11780) metW 2560997..2561617 (-) 621 WP_003213768.1 methionine biosynthesis protein MetW -
  ACM530_RS11785 (ACM530_11785) metX 2561625..2562764 (-) 1140 WP_416364821.1 homoserine O-succinyltransferase MetX -
  ACM530_RS11790 (ACM530_11790) - 2562967..2563554 (-) 588 WP_145157732.1 YggT family protein -
  ACM530_RS11795 (ACM530_11795) proC 2563590..2564408 (-) 819 WP_416364822.1 pyrroline-5-carboxylate reductase -
  ACM530_RS11800 (ACM530_11800) - 2564442..2565128 (-) 687 WP_416364823.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM530_RS11805 (ACM530_11805) pilT 2565186..2566220 (+) 1035 WP_416364824.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM530_RS11810 (ACM530_11810) - 2566361..2567002 (-) 642 WP_145158570.1 C40 family peptidase -
  ACM530_RS11815 (ACM530_11815) - 2567252..2567680 (+) 429 WP_416364825.1 NINE protein -
  ACM530_RS11820 (ACM530_11820) - 2567832..2569103 (-) 1272 WP_145157722.1 dihydroorotase -
  ACM530_RS11825 (ACM530_11825) - 2569100..2570104 (-) 1005 WP_159960639.1 aspartate carbamoyltransferase catalytic subunit -
  ACM530_RS11830 (ACM530_11830) pyrR 2570129..2570635 (-) 507 WP_106575674.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACM530_RS11835 (ACM530_11835) ruvX 2570708..2571145 (-) 438 WP_003195067.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37412.29 Da        Isoelectric Point: 6.9839

>NTDB_id=1099781 ACM530_RS11805 WP_416364824.1 2565186..2566220(+) (pilT) [Pseudomonas sp. NFX183]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEVWPLEGPVLTPPQVAELLSPLLNRYQQKDFETSLETDFAFELPSVAR
FRVNVFQQDRGMGAVFRTIPAEVQSLERLGLGAVFQRIAQLPCGLVLVTGPTGSGKSTTLAAMIDYLNGHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKSVDRL
VDVFPAAEKPMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVSEGLISREDARGQARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1099781 ACM530_RS11805 WP_416364824.1 2565186..2566220(+) (pilT) [Pseudomonas sp. NFX183]
ATGGATATCACTGAATTGCTGACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCGGCCGGCCTGGCACCGAT
GCTGCGTGTCGATGGCGAGGTCTGGCCGCTGGAGGGCCCGGTGCTCACGCCCCCGCAAGTTGCGGAGTTATTGAGCCCTT
TGCTCAACCGCTACCAACAAAAGGATTTCGAAACATCTCTTGAAACCGATTTTGCCTTCGAACTGCCGAGCGTGGCGCGG
TTCCGGGTGAATGTATTTCAACAGGATCGCGGCATGGGGGCGGTGTTTCGCACCATTCCCGCCGAAGTGCAGAGCCTTGA
ACGCCTCGGGCTGGGGGCGGTGTTCCAGCGTATCGCGCAACTGCCGTGCGGCCTGGTGCTGGTCACCGGGCCGACCGGGT
CGGGCAAATCCACCACTTTGGCGGCGATGATCGACTACCTTAACGGGCATCGGCGCCAGCACATCCTCACCCTCGAAGAC
CCCATCGAATTTATCCACAGGCCGAAAATGGCCTTGATCAACCAGCGTCAGGTGCACCGCGATACCCACAGCTTTTCGGC
GGCGCTGCGTTCGGCGCTGCGTGAAGACCCGGATGTGATTCTGGTGGGCGAGTTGCGCGACCTTGAGACCATCCGCCTGG
CGCTGACGGCCGCTGAGACCGGGCACCTGGTATTTGGCACCTTGCACACCACCTCGGCCGCAAAGAGCGTGGACCGGCTG
GTGGACGTGTTCCCGGCAGCGGAAAAGCCCATGGTGCGCTCGATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCGCAAGT
GCTGGTCAAGAAGATTGGCGGTGGGCGGGTGGCGGCTCACGAAATCATGCTGGGTACACCGGCTATTCGTAACCTGATCC
GTGAAGACAAAGTGGCGCAGATGGTCTCGGCGATCCAGACCGGTGGGGCGCTGGGGATGAAAACGTTGGACATGAGCTTG
AAGGCGTTGGTCAGCGAGGGGCTGATCAGCCGGGAAGATGCGCGGGGGCAGGCGAGGGTGCCTGGGGACATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.314

99.128

0.727
  pilT Pseudomonas stutzeri DSM 10701

71.261

99.128

0.706
  pilT Acinetobacter nosocomialis M2

70.381

99.128

0.698
  pilT Acinetobacter baumannii D1279779

70.088

99.128

0.695
  pilT Acinetobacter baumannii strain A118

70.088

99.128

0.695
  pilT Acinetobacter baylyi ADP1

69.501

99.128

0.689
  pilT Legionella pneumophila strain Lp02

64.201

98.256

0.631
  pilT Legionella pneumophila strain ERS1305867

64.201

98.256

0.631
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.095

97.965

0.628
  pilT Vibrio cholerae strain A1552

64.095

97.965

0.628
  pilT Neisseria meningitidis 8013

61.765

98.837

0.61
  pilT Neisseria gonorrhoeae MS11

61.471

98.837

0.608
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.45

96.802

0.488
  pilU Pseudomonas stutzeri DSM 10701

39.42

100

0.395
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384

Multiple sequence alignment