Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ACGHAD_RS03785 Genome accession   NZ_AP031500
Coordinates   895929..896354 (+) Length   141 a.a.
NCBI ID   WP_382413764.1    Uniprot ID   -
Organism   Gilvimarinus japonicus strain 12-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 890929..901354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHAD_RS03765 (R50076_07510) coaE 891122..891742 (-) 621 WP_339616426.1 dephospho-CoA kinase -
  ACGHAD_RS03770 (R50076_07520) pilD 891755..892630 (-) 876 WP_382413774.1 prepilin peptidase Machinery gene
  ACGHAD_RS03775 (R50076_07530) pilC 892635..893894 (-) 1260 WP_382413769.1 type II secretion system F family protein Machinery gene
  ACGHAD_RS03780 (R50076_07540) pilB 893898..895607 (-) 1710 WP_382413766.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGHAD_RS03785 (R50076_07550) pilA2 895929..896354 (+) 426 WP_382413764.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACGHAD_RS03790 (R50076_07560) - 896468..897178 (+) 711 WP_382413762.1 hypothetical protein -
  ACGHAD_RS03795 (R50076_07570) nadC 897311..898174 (-) 864 WP_382413760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACGHAD_RS03800 (R50076_07580) - 898446..900794 (+) 2349 WP_382413758.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14564.58 Da        Isoelectric Point: 4.3611

>NTDB_id=109953 ACGHAD_RS03785 WP_382413764.1 895929..896354(+) (pilA2) [Gilvimarinus japonicus strain 12-2]
MKKQQGFTLIELMIVIAIIGILAAIALPAYQNYTIRTKNAECLSIAASPKLAVAETFVSNGGEDWPGTLAAAGYVPAATN
YCNEVAYTGGGVFTVTSKDTGGAVTYTFTPTVVGGADSPSAIEWVCEGAGDPAHMPSECRP

Nucleotide


Download         Length: 426 bp        

>NTDB_id=109953 ACGHAD_RS03785 WP_382413764.1 895929..896354(+) (pilA2) [Gilvimarinus japonicus strain 12-2]
ATGAAAAAACAACAAGGCTTTACACTGATTGAATTGATGATCGTTATTGCGATCATCGGTATTCTGGCGGCAATTGCGCT
GCCTGCTTACCAAAATTACACCATTCGTACTAAAAACGCCGAGTGTTTGAGCATTGCTGCGTCCCCTAAGTTGGCAGTTG
CGGAAACATTTGTGTCCAATGGTGGTGAAGATTGGCCAGGTACTTTAGCTGCTGCTGGGTATGTCCCCGCTGCGACTAAT
TACTGCAACGAGGTTGCTTATACAGGTGGTGGAGTATTTACGGTTACGTCAAAAGATACCGGTGGAGCGGTAACCTATAC
ATTCACCCCTACGGTTGTGGGGGGAGCTGACTCGCCTTCCGCTATTGAGTGGGTTTGTGAGGGGGCGGGTGATCCAGCTC
ATATGCCTTCAGAGTGTCGTCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

49.645

100

0.496
  pilA2 Legionella pneumophila strain ERS1305867

49.645

100

0.496
  comP Acinetobacter baylyi ADP1

42.105

100

0.454
  pilA Ralstonia pseudosolanacearum GMI1000

40.816

100

0.426
  pilA/pilA1 Eikenella corrodens VA1

38.312

100

0.418
  pilE Neisseria gonorrhoeae MS11

36.709

100

0.411
  pilE Neisseria gonorrhoeae strain FA1090

36.306

100

0.404
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.621

100

0.397
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.129

98.582

0.376
  pilA Vibrio cholerae strain A1552

34.694

100

0.362
  pilA Vibrio cholerae C6706

34.694

100

0.362
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.694

100

0.362
  pilA Vibrio parahaemolyticus RIMD 2210633

44.737

80.851

0.362

Multiple sequence alignment