Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGHAD_RS03780 Genome accession   NZ_AP031500
Coordinates   893898..895607 (-) Length   569 a.a.
NCBI ID   WP_382413766.1    Uniprot ID   -
Organism   Gilvimarinus japonicus strain 12-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 888898..900607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHAD_RS03755 (R50076_07500) - 889933..890862 (+) 930 WP_382413781.1 Nudix family hydrolase -
  ACGHAD_RS03760 yacG 890916..891104 (-) 189 WP_339616425.1 DNA gyrase inhibitor YacG -
  ACGHAD_RS03765 (R50076_07510) coaE 891122..891742 (-) 621 WP_339616426.1 dephospho-CoA kinase -
  ACGHAD_RS03770 (R50076_07520) pilD 891755..892630 (-) 876 WP_382413774.1 prepilin peptidase Machinery gene
  ACGHAD_RS03775 (R50076_07530) pilC 892635..893894 (-) 1260 WP_382413769.1 type II secretion system F family protein Machinery gene
  ACGHAD_RS03780 (R50076_07540) pilB 893898..895607 (-) 1710 WP_382413766.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGHAD_RS03785 (R50076_07550) pilA2 895929..896354 (+) 426 WP_382413764.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACGHAD_RS03790 (R50076_07560) - 896468..897178 (+) 711 WP_382413762.1 hypothetical protein -
  ACGHAD_RS03795 (R50076_07570) nadC 897311..898174 (-) 864 WP_382413760.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62014.12 Da        Isoelectric Point: 4.9472

>NTDB_id=109952 ACGHAD_RS03780 WP_382413766.1 893898..895607(-) (pilB) [Gilvimarinus japonicus strain 12-2]
MAALNGLAKRLVNDGLLNETLASSSLAEANKQKMPFVVYLVQNGVLDARTLALVAADEFGTPVFDLKAINPQTLPSKLVD
AKLIEKHRALPLIRRGNRLFVGVVDPTNLHALDEIKFNTGINTEAVLVEADKLAIAIEAYINANEESIGDALGGLDDEDL
DNLDTENGAPTEDATVNEADEAPIVRFINKILLDAIKIGASDIHFEPYEKTYRVRLRTDGILQEVSHPPTNLATRIAARL
KVMSQMDISERRVPQDGRIKMKISKTRAIDFRVNTLPTLFGEKIVLRILDPSSAKLGIDALGYEDEQKQMFMDALNQPQG
MILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVQMNTKVGLTFAEALRSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSLIIAQRLARRLCPRCKKPASDIPSEILAEEGFD
NIGIPREELELYHPVGCDSCLKGYKGRVGVYEVVRITPAISSLIMEGGNSLQIAKAATEAGFNNLRISALRKAAQGLTSL
EEANRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=109952 ACGHAD_RS03780 WP_382413766.1 893898..895607(-) (pilB) [Gilvimarinus japonicus strain 12-2]
ATGGCAGCACTAAACGGACTGGCAAAACGACTGGTTAATGACGGTCTACTGAACGAGACATTGGCCAGCAGTAGCTTAGC
GGAGGCCAACAAACAAAAAATGCCTTTCGTGGTTTACTTGGTACAAAATGGGGTTTTGGACGCCAGAACGCTGGCATTGG
TAGCGGCCGATGAGTTTGGCACCCCAGTCTTTGACCTAAAAGCTATTAACCCCCAAACGCTCCCCAGTAAGTTGGTAGAT
GCCAAGTTGATTGAAAAACACCGTGCACTACCTCTTATTCGGCGCGGCAATCGGCTTTTTGTGGGAGTCGTCGACCCCAC
CAACTTGCATGCGCTTGATGAGATCAAATTTAATACCGGAATTAATACTGAGGCCGTTTTAGTAGAGGCAGACAAATTAG
CCATAGCGATAGAAGCTTATATTAACGCCAACGAAGAGAGCATTGGCGATGCACTTGGCGGCTTAGATGACGAAGACCTA
GACAATTTAGACACCGAGAATGGCGCCCCCACAGAAGACGCTACTGTTAACGAGGCTGATGAGGCCCCTATAGTACGCTT
CATCAACAAGATACTGCTAGATGCCATTAAAATCGGCGCCTCAGACATTCACTTTGAGCCGTACGAGAAAACCTACCGTG
TAAGACTGCGTACTGATGGTATTTTACAAGAAGTGTCCCACCCACCCACCAACCTTGCCACACGCATTGCTGCGCGCCTT
AAGGTGATGTCGCAAATGGACATATCCGAGCGCCGTGTACCACAAGACGGCCGGATTAAGATGAAGATATCCAAGACGCG
CGCCATCGACTTTAGGGTAAACACTCTACCCACCCTGTTTGGCGAGAAGATCGTCCTGCGGATTTTGGACCCAAGCTCAG
CCAAGCTGGGCATTGACGCTCTAGGGTATGAAGACGAGCAGAAACAAATGTTCATGGATGCCCTGAACCAACCGCAAGGT
ATGATTTTGGTCACAGGTCCTACTGGCAGCGGTAAAACCGTATCGCTTTATACTGGCCTAAATATTCTAAATACCGAAGA
ACGCAACATCTCTACAGCCGAAGACCCAGTGGAGATCAACTTAGAGGGTATTAATCAGGTACAAATGAATACCAAAGTGG
GCCTAACCTTTGCCGAGGCACTGCGCTCGTTTTTGCGTCAAGACCCGGATATCGTGATGGTAGGGGAGATTCGCGACCTA
GAAACCGCCGAGATTGCCATTAAAGCAGCACAAACTGGCCATTTAGTATTGTCCACCCTTCACACTAACAGCGCCCCTGA
GACCCTAACACGTTTACTTAACATGGGCGTACCAGCGTTTAACGTGGCTACCAGCGTTAGTTTAATTATTGCTCAGCGCC
TGGCGCGGCGGCTGTGCCCAAGATGCAAAAAGCCAGCCAGCGATATACCCAGCGAGATTTTAGCCGAAGAGGGCTTTGAC
AATATTGGCATACCACGAGAGGAGCTTGAACTATACCACCCAGTAGGCTGTGATTCCTGCCTGAAAGGTTATAAAGGTCG
AGTCGGGGTGTATGAAGTAGTTCGCATTACTCCTGCTATATCAAGCCTTATAATGGAAGGGGGGAACTCGCTGCAAATAG
CCAAAGCCGCTACTGAAGCGGGATTTAACAATTTAAGAATCTCGGCGCTGCGCAAAGCAGCTCAAGGGCTAACCAGCCTA
GAAGAAGCCAACCGAGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.16

100

0.612
  pilB Acinetobacter baylyi ADP1

60.105

100

0.601
  pilB Legionella pneumophila strain ERS1305867

56.491

100

0.566
  pilB Vibrio campbellii strain DS40M4

50.874

100

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50.977

98.946

0.504
  pilB Vibrio cholerae strain A1552

51.698

93.146

0.482
  pilF Neisseria gonorrhoeae MS11

49.084

95.958

0.471
  pilB/pilB1 Synechocystis sp. PCC 6803

41.292

89.807

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.764

93.849

0.364

Multiple sequence alignment