Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACM0CX_RS20050 Genome accession   NZ_CP180564
Coordinates   4361283..4362317 (+) Length   344 a.a.
NCBI ID   WP_005305010.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BJ018     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4356283..4367317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0CX_RS20020 - 4357962..4358273 (+) 312 WP_207137359.1 hypothetical protein -
  ACM0CX_RS20025 - 4358330..4358749 (-) 420 WP_011707384.1 DUF4426 domain-containing protein -
  ACM0CX_RS20030 yggU 4358768..4359067 (-) 300 WP_011707385.1 DUF167 family protein YggU -
  ACM0CX_RS20035 - 4359067..4359618 (-) 552 WP_017411214.1 YggT family protein -
  ACM0CX_RS20040 proC 4359659..4360483 (-) 825 WP_411637471.1 pyrroline-5-carboxylate reductase -
  ACM0CX_RS20045 - 4360542..4361243 (-) 702 WP_411637470.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM0CX_RS20050 pilT 4361283..4362317 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  ACM0CX_RS20055 pilU 4362346..4363455 (+) 1110 WP_411637469.1 type IVa pilus ATPase TapU Machinery gene
  ACM0CX_RS20060 yaaA 4363487..4364260 (+) 774 WP_411637468.1 peroxide stress protein YaaA -
  ACM0CX_RS20065 srmB 4364437..4365660 (-) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  ACM0CX_RS20070 - 4365789..4366499 (+) 711 WP_411637467.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38319.12 Da        Isoelectric Point: 6.7439

>NTDB_id=1096650 ACM0CX_RS20050 WP_005305010.1 4361283..4362317(+) (pilT) [Aeromonas hydrophila strain BJ018]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGMVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096650 ACM0CX_RS20050 WP_005305010.1 4361283..4362317(+) (pilT) [Aeromonas hydrophila strain BJ018]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTGGACTTCTCGTTCGAGGTGCCGAACCTCGCCCGC
TTCCGGGTCAACGCCTTCCAGCAGGCACGCGGCTCCGGCGCGGTATTTCGGACCATCCCCAGCACGGTGCTGAGCCTCGA
AGATCTGGACGCCCCGGAGATCTTTCGCAAGATTGCCGAATACCCCCGCGGTCTGGTACTGGTGACCGGCCCGACCGGCT
CGGGCAAATCCACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATCCTCACCATTGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGTGACCTGGAAACCATTCGTCTGG
CCATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTCTCCGAGTCGCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGCGGCGGCCGGGTGGCGGCCCACGAAATCATGATGGGCATTCCCGCCGTACGAAACCTCATCC
GGGAAGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGCGGCATGGTGGCGGCGCTCGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment