Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXYU_RS01475 Genome accession   NZ_CP180551
Coordinates   324581..325615 (+) Length   344 a.a.
NCBI ID   WP_415899879.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD48_11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 319581..330615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYU_RS01445 (ACMXYU_01445) - 319786..320235 (-) 450 WP_415899874.1 DUF4426 domain-containing protein -
  ACMXYU_RS01450 (ACMXYU_01450) metW 320248..320838 (-) 591 WP_415893171.1 methionine biosynthesis protein MetW -
  ACMXYU_RS01455 (ACMXYU_01455) metX 320838..322007 (-) 1170 WP_415899875.1 homoserine O-succinyltransferase MetX -
  ACMXYU_RS01460 (ACMXYU_01460) - 322304..322849 (-) 546 WP_415899876.1 YggT family protein -
  ACMXYU_RS01465 (ACMXYU_01465) proC 322948..323775 (-) 828 WP_415899877.1 pyrroline-5-carboxylate reductase -
  ACMXYU_RS01470 (ACMXYU_01470) - 323794..324489 (-) 696 WP_415899878.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYU_RS01475 (ACMXYU_01475) pilT 324581..325615 (+) 1035 WP_415899879.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYU_RS01480 (ACMXYU_01480) pilU 325638..326804 (+) 1167 WP_415886689.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYU_RS01485 (ACMXYU_01485) - 326880..328154 (-) 1275 WP_415899880.1 dihydroorotase -
  ACMXYU_RS01490 (ACMXYU_01490) - 328160..329164 (-) 1005 WP_415899881.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYU_RS01495 (ACMXYU_01495) pyrR 329174..329689 (-) 516 WP_415899882.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYU_RS01500 (ACMXYU_01500) ruvX 329670..330146 (-) 477 WP_415899883.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38299.95 Da        Isoelectric Point: 6.6681

>NTDB_id=1096484 ACMXYU_RS01475 WP_415899879.1 324581..325615(+) (pilT) [Neptuniibacter sp. QD48_11]
MDITELLSFTVQQGASDLHISAGMPPIIRVDGDVRRIKLPSLDHKQVHTLIYDIMNDKQRKEYEDVLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLDMGKVFKDLSEKPRGLVLVTGPTGSGKSTTLAAMIDYINDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLMGVISQTLLKKPKGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGASFGMKTLDQSL
QELMQKGFITRETARERAANPQQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096484 ACMXYU_RS01475 WP_415899879.1 324581..325615(+) (pilT) [Neptuniibacter sp. QD48_11]
ATGGATATTACCGAACTTTTATCATTTACCGTACAACAAGGTGCATCAGATTTGCATATATCAGCAGGGATGCCACCTAT
TATTCGTGTTGACGGTGATGTGCGTCGCATAAAACTGCCTTCTTTAGACCATAAACAGGTTCATACTCTCATTTACGACA
TTATGAATGATAAACAACGTAAAGAGTATGAAGATGTACTTGAGACAGACTTTTCTTTCGAGGTGCCTGGTTTAGCGCGT
TTTCGTGTGAACGCATTTAACCAGAATCGCGGTGCGGGTGCTGTTTTCCGAACTATCCCTAGTAAGGTTTTAACGTTAGA
TGATCTCGATATGGGTAAGGTGTTTAAGGACCTTTCTGAGAAGCCACGTGGCTTAGTTCTGGTTACGGGTCCTACAGGTT
CTGGTAAAAGTACAACATTGGCTGCAATGATCGATTACATTAATGACACGCGTGCCGATCATATTTTGACTATTGAAGAC
CCAATTGAATTCGTGCATGAAAGTAAGAAGTGCTTGGTAAACCAGCGTGAAGTGCATAGGGATACACATAGCTTTTCGAA
TGCACTTCGTTCGGCACTACGTGAAGACCCGGATATCATCCTTGTCGGTGAGATGCGAGACTTGGAAACTATCCGTCTCG
CGCTGACTGCCGCTGAAACCGGCCATTTAGTATTTGGTACTTTACACACCACCTCAGCAGCTAAAACAATTGACCGTGTT
ATTGACGTATTTCCGGCCGCTGAGAAGGATATGGTGAGATCCATGCTTTCAGAGTCGCTGATGGGTGTGATCTCGCAGAC
ACTTTTGAAAAAGCCGAAAGGCGGCCGGGTTGCAGCTCATGAGATTATGATTGGTACCCCAGCTATTCGTAACCTTATCC
GAGAGGACAAAGTTGCTCAGATGTATTCGGCGATCCAGACTGGTGCATCATTTGGTATGAAAACTTTGGATCAATCTCTG
CAAGAATTGATGCAAAAAGGATTTATAACACGCGAAACAGCGCGTGAGCGTGCAGCAAACCCACAACAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.849

100

0.828
  pilT Pseudomonas stutzeri DSM 10701

81.686

100

0.817
  pilT Acinetobacter nosocomialis M2

80.814

100

0.808
  pilT Acinetobacter baumannii D1279779

80.814

100

0.808
  pilT Acinetobacter baumannii strain A118

80.814

100

0.808
  pilT Acinetobacter baylyi ADP1

79.942

100

0.799
  pilT Legionella pneumophila strain Lp02

73.256

100

0.733
  pilT Legionella pneumophila strain ERS1305867

73.256

100

0.733
  pilT Neisseria meningitidis 8013

66.472

99.709

0.663
  pilT Neisseria gonorrhoeae MS11

66.181

99.709

0.66
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.997

95.64

0.66
  pilT Vibrio cholerae strain A1552

68.997

95.64

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment