Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXYS_RS01465 Genome accession   NZ_CP180549
Coordinates   342008..343042 (+) Length   344 a.a.
NCBI ID   WP_415897399.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD57_21     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337008..348042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYS_RS01435 (ACMXYS_01435) - 337202..337651 (-) 450 WP_415897394.1 DUF4426 domain-containing protein -
  ACMXYS_RS01440 (ACMXYS_01440) metW 337664..338254 (-) 591 WP_415897395.1 methionine biosynthesis protein MetW -
  ACMXYS_RS01445 (ACMXYS_01445) metX 338254..339423 (-) 1170 WP_415897396.1 homoserine O-succinyltransferase MetX -
  ACMXYS_RS01450 (ACMXYS_01450) - 339720..340265 (-) 546 WP_415883219.1 YggT family protein -
  ACMXYS_RS01455 (ACMXYS_01455) proC 340375..341202 (-) 828 WP_415897397.1 pyrroline-5-carboxylate reductase -
  ACMXYS_RS01460 (ACMXYS_01460) - 341221..341916 (-) 696 WP_415897398.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYS_RS01465 (ACMXYS_01465) pilT 342008..343042 (+) 1035 WP_415897399.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYS_RS01470 (ACMXYS_01470) pilU 343065..344231 (+) 1167 WP_415897400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYS_RS01475 (ACMXYS_01475) - 344292..345566 (-) 1275 WP_415897401.1 dihydroorotase -
  ACMXYS_RS01480 (ACMXYS_01480) - 345572..346576 (-) 1005 WP_415897402.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYS_RS01485 (ACMXYS_01485) pyrR 346586..347101 (-) 516 WP_415897403.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYS_RS01490 (ACMXYS_01490) ruvX 347082..347558 (-) 477 WP_415883227.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38214.85 Da        Isoelectric Point: 6.5797

>NTDB_id=1096438 ACMXYS_RS01465 WP_415897399.1 342008..343042(+) (pilT) [Neptuniibacter sp. QD57_21]
MDITELLSFTVQQGASDLHISAGMPPIIRVDGDVRRIKLPSLDHKQVHTLVYDIMNDKQRKEYEDVLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLDMGKVFQDLSEKPRGLVLVTGPTGSGKSTTLAAMVDYINDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFAHALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLMGVISQTLLKKPKGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGAAFGMKTLDQSL
QELMQKGLISRETARERAANPQQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096438 ACMXYS_RS01465 WP_415897399.1 342008..343042(+) (pilT) [Neptuniibacter sp. QD57_21]
ATGGATATTACCGAACTTTTATCATTTACCGTACAACAAGGTGCATCTGACTTGCATATTTCTGCAGGAATGCCCCCTAT
TATTCGTGTTGACGGTGATGTACGTCGTATAAAACTGCCTTCATTGGATCATAAGCAAGTTCACACACTTGTCTATGACA
TCATGAACGATAAACAGCGTAAAGAGTACGAAGATGTGCTCGAAACGGATTTCTCGTTCGAAGTCCCTGGTTTGGCGCGC
TTTCGTGTAAACGCATTTAACCAAAACCGTGGTGCAGGTGCTGTTTTCCGTACGATCCCAAGTAAGGTTTTGACTTTAGA
TGATCTTGATATGGGTAAGGTTTTTCAGGATCTGTCTGAAAAGCCGCGTGGATTAGTGCTTGTGACGGGCCCTACTGGGT
CCGGTAAAAGTACCACCTTGGCTGCGATGGTCGATTACATCAATGATACTCGTGCAGATCATATTTTAACGATCGAAGAT
CCAATCGAATTCGTGCACGAAAGTAAAAAATGCTTGGTTAACCAGCGTGAAGTGCATCGAGATACTCATAGCTTTGCACA
TGCGCTTCGATCGGCACTTCGTGAAGACCCGGATATTATCCTTGTAGGTGAGATGCGAGATTTGGAGACTATCCGTCTTG
CGCTTACTGCTGCTGAAACAGGTCACTTAGTGTTTGGTACTTTGCATACGACCTCTGCAGCAAAAACAATTGACCGTGTG
ATTGATGTATTCCCAGCGGCTGAGAAAGATATGGTGCGATCCATGCTCTCAGAGTCATTGATGGGTGTGATATCGCAAAC
ACTGTTGAAAAAGCCAAAAGGCGGGCGAGTTGCTGCTCACGAAATTATGATTGGTACACCGGCTATACGTAACCTTATCC
GTGAAGATAAAGTAGCGCAGATGTATTCGGCGATCCAAACGGGTGCAGCATTTGGTATGAAAACGTTGGATCAATCTCTA
CAAGAGTTGATGCAGAAAGGATTAATCTCGCGCGAGACAGCCCGTGAGCGAGCAGCCAACCCACAACAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.267

100

0.823
  pilT Pseudomonas stutzeri DSM 10701

81.395

100

0.814
  pilT Acinetobacter nosocomialis M2

81.105

100

0.811
  pilT Acinetobacter baumannii D1279779

81.105

100

0.811
  pilT Acinetobacter baumannii strain A118

81.105

100

0.811
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

72.965

100

0.73
  pilT Legionella pneumophila strain ERS1305867

72.965

100

0.73
  pilT Neisseria meningitidis 8013

67.347

99.709

0.672
  pilT Neisseria gonorrhoeae MS11

67.055

99.709

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.359

97.965

0.66
  pilT Vibrio cholerae strain A1552

67.359

97.965

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.506
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment